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李洁花,廖晨希,杨盾,曾友,黄丹,张熙,刘春华,谢明霞.基于古汉愈疡方的不同剂型对糖尿病大鼠溃疡创面愈合疗效的比较研究[J].湖南中医药大学学报,2022,42(4):548-556[点击复制] |
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| 基于古汉愈疡方的不同剂型对糖尿病大鼠溃疡创面愈合疗效的比较研究 |
| 李洁花,廖晨希,杨盾,曾友,黄丹,张熙,刘春华,谢明霞 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 观察“古汉愈疡方”对糖尿病皮肤溃疡大鼠创面的修复作用，探讨其促进创面愈合的作用机制，并比较其凝胶剂与喷雾剂的疗效。方法 将80只SPF级健康成年雄性SD大鼠，随机分为对照组（15只）和糖尿病组（65只），糖尿病组大鼠采用一次性腹腔注射链脲佐菌素诱导糖尿病模型，造模成功的60只糖尿病大鼠与15只对照组的正常大鼠一起制作皮肤溃疡模型，对照组予凡士林纱布换药，糖尿病组大鼠再随机分为模型组、古汉愈疡方喷雾治疗组（以下简称喷雾组）、古汉愈疡方凝胶治疗组（以下简称凝胶组）、康复新液治疗组（以下简称康复新组），每组15只，分别予以凡士林纱布、古汉愈疡方喷雾剂、古汉愈疡方凝胶、康复新液干预，每日换药1次，持续21 d。肉眼观察各组大鼠创面愈合情况，并计算3、7、14、21 d动态创面收缩率，HE染色观察大鼠创面组织病理学变化，免疫组化检测胰岛素样生长因子-1(insulin like growth factor-1, IGF-1)及血管内皮生长因子(vascular endothelial growth factor, VEGF)的蛋白表达。结果（1）给药3、7、14、21 d时，对照组、喷雾组、凝胶组和康复新组创面收缩率均显著高于模型组(P<0.01)，凝胶组显著高于喷雾组(P<0.01)；给药21 d时，凝胶组与康复新组、对照组的创面收缩率差异无统计学意义(P>0.05)。（2）HE染色显示，模型组创面化脓，有炎性渗出液，创面缩小缓慢；相较模型组，对照组和凝胶组新鲜肉芽组织明显增生，毛细血管生长迅速，成纤维细胞数量明显增多，炎症细胞浸润明显减少，喷雾组和康复新组次之。（3）免疫组化结果显示，与模型组比较，其余4组在各时间点创面组织中IGF-1的蛋白表达水平显著升高(P<0.05或P<0.01)；与对照组比较，凝胶组各时间点IGF-1的蛋白表达水平升高(P<0.05或P<0.01)，而7、21 d喷雾组与康复新组IGF-1的蛋白表达水平降低(P<0.05或P<0.01)，14 d对照组、喷雾组与康复新组差异无统计学意义(P>0.05)；与凝胶组比较，喷雾组与康复新组在各时间点IGF-1的蛋白表达降低(P<0.01)。与模型组比较，其余4组在各时间点创面组织中VEGF的蛋白表达水平显著升高(P<0.05或P<0.01)；与对照组比较，喷雾组、凝胶组与康复新组VEGF的蛋白表达水平差异无统计学意义(P>0.05)；与凝胶组比较，康复新组VEGF的蛋白表达水平在14 d偏低(P<0.05)，喷雾组VEGF的蛋白表达水平在21 d偏低(P<0.05)。结论 “古汉愈疡方”可有效促进糖尿病皮肤溃疡大鼠创面的修复，其疗效机制可能与上调IGF-1及VEGF的表达有关，且凝胶剂比喷雾剂疗效更佳。 |
| 关键词: 糖尿病 古汉愈疡方 皮肤溃疡 胰岛素样生长因子 血管内皮生长因子 大鼠 |
| DOI：10.3969/j.issn.1674－070X.2022.04.006 |
| 投稿时间：2021-09-30 |
| 基金项目:湖南省自然科学基金项目（2021JJ80060）；湖南省大学生创新基金项目（20212450，20202626）；湖南中医药大学校级科研基金项目（2019XJJ004，2018XJJ26）。 |
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| Comparative study on the therapeutic effect of different dosage forms of Guhan Yuyang Recipe on ulcer wound healing in diabetic rats |
| LI Jiehua,LIAO Chenxi,YANG Dun,ZENG You,HUANG Dan,ZHANG Xi,LIU Chunhua,XIE Mingxia |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To observe the effect of “Guhan Yuyang Recipe” on wound healing of diabetic skin ulcer rats, to explore its mechanism of promoting wound healing, and to compare the curative effect of gel and spray. Methods 80 SPF grade healthy adult male SD rats were randomly divided into control group (n=15) and diabetic group (n=65). The diabetic model was induced by intraperitoneal injection of streptozotocin. 60 diabetic rats successfully established and 15 normal rats in the control group were used to make skin ulcer model, and the control group was treated with vaseline gauze. Diabetic rats were randomly divided into model group, Guhan Yuyang Recipe spray treatment group (hereinafter referred to as spray group), Guhan Yuyang Recipe gel treatment group (hereinafter referred to as gel group) and Kangfuxin liquid treatment group (hereinafter referred to as Kangfuxin group), with 15 rats in each group. The rats were treated with vaseline gauze, Guhan Yuyang Recipe spray, Guhan Yuyang Recipe gel and Kangfuxin liquid respectively, and the dressing was changed once a day for 21 days. The wound healing of rats in each group was observed with naked eye, and the dynamic wound contraction rate at 3rd, 7th, 14th and 21st day was calculated. The histopathological changes of wounds were observed by HE staining, and the protein expression levels of insulin like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) were detected by immunohistochemistry. Results (1) On the 3rd, 7th, 14th and 21st day after administration, the wound contraction rate in the control group, spray group, gel group and Kangfuxin group was significantly higher than that in the model group (P<0.01), and that in the gel group was significantly higher than that in the spray group (P<0.01); on the 21st day of administration, there was no significant difference in wound contraction rate between the gel group, the Kangfuxin group and the control group (P>0.05). (2) HE staining showed that the wound in the model group suppurated, there was inflammatory exudate, and the wound shrank slowly; compared with the model group, fresh granulation tissue proliferated significantly, capillaries grew rapidly, fibroblasts increased and inflammatory cell infiltration decreased significantly in the control group and gel group, followed by spray group and Kangfuxin group. (3) The results of immunohistochemistry showed that, compared with the model group, the protein expression level of IGF-1 in the other four groups increased significantly at each time point (P<0.05 or P<0.01); compared with the control group, the protein expression level of IGF-1 in the gel group was higher at each time point (P<0.05 or P<0.01), on the 7th and 21st day, the protein expression level of IGF-1 in spray group and Kangfuxin group was lower than that in control group (P<0.05 or P<0.01), on the 14th day, there was no significant difference among control group, spray group and Kangfuxin group (P>0.05); compared with gel group, the protein expression of IGF-1 in spray group and Kangfuxin group was significantly decreased at each time point (P<0.01). Compared with the model group, the protein expression level of VEGF in the wound tissue of the other four groups increased significantly at each time point (P<0.05 or P<0.01); compared with the control group, there was no significant difference in the protein expression level of VEGF among spray group, gel group and Kangfuxin group (P>0.05); compared with gel group, the protein expression level of VEGF in Kangfuxin group was lower on the 14th day (P<0.05), and that of VEGF in the spray group on the 21st day (P<0.05). Conclusion "Guhan Yuyang Recipe" can effectively promote the wound repair of diabetic skin ulcer rats. The mechanism of its curative effect may be related to the up-regulation of the expression of IGF-1 and VEGF, and the curative effect of gel is better than that of spray. |
| Key words: diabetes mellitus Guhan Yuyang Recipe skin ulcer insulin-like growth factor vascular endothelial growth factor rat |
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