| 引用本文: |
唐总德,周琦人,李冰杰,刘健康,贺福元,朱志飞,周晋.经典名方身痛逐瘀汤物质基准的制备工艺研究[J].湖南中医药大学学报,2022,42(2):240-245[点击复制] |
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| 经典名方身痛逐瘀汤物质基准的制备工艺研究 |
| 唐总德,周琦人,李冰杰,刘健康,贺福元,朱志飞,周晋 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湖南中医药大学药学院, 湖南 长沙 410208;中药成药性与制剂制备湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学中医药超分子机理与数理特征化实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 对身痛逐瘀汤的具体制备工艺展开研究，为其物质基准进一步研究提供科学性的基础。方法 对身痛逐瘀汤煎煮、过滤、浓缩及干燥等一系列制备工艺过程进行单因素考察，对进行单因素考察后的样品进行出膏率、浸出物以及指纹图谱特征图谱的含量测定，并作为关键指标参数，确定最佳制备工艺。结果 身痛逐瘀汤物质基准的制备工艺参照《医林改错》中身痛逐瘀汤处方，一钱为3.73 g，精密称定全方共93.25 g，加入10倍量水，浸泡30 min，武火煮沸后加热回流提取1 h，趁热用200目纱布过滤，加入一定体积95%乙醇至含醇量为80%，放置24 h以上，抽滤，回收上清液，冷冻干燥，得冻干粉。工艺验证结果表明该制备工艺稳定。结论 本实验得出的身痛逐瘀汤的制备工艺稳定可行，将对身痛逐瘀汤物质基础的研究提供依据，并为古代经典名方复方研究奠定基础。 |
| 关键词: 经典名方 身痛逐瘀汤 物质基准 制备工艺 医林改错 |
| DOI：10.3969/j.issn.1674-070X.2022.02.012 |
| 投稿时间：2021-04-09 |
| 基金项目:国家自然科学基金项目（81903759）；湖南省教育厅项目（18K071，21A0256）；国家留学基金项目（201708430243）；长沙市科学技术局项目（kq2004059）；湖南中医药大学药学一流学科开放基金项目（2021YX16）；湖南省大学生创新训练计划项目（S202110541070）；湖南省研究生科研创新项目（CX20210711）。 |
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| Study on the preparation process of substance standard of classic prescription Shentong Zhuyu Decoction |
| TANG Zongde,ZHOU Qiren,LI Bingjie,LIU Jiankang,HE Fuyuan,ZHU Zhifei,ZHOU Jin |
| (College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Key Laboratory of Druggability and Preparation Modification of TCM, Changsha, Hunan 410208, China;Laboratory of Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the specific preparation technology of Shentong Zhuyu Decoction and provide scientific basis for further study of its substance standard. Methods A series of preparation process of Shentong Zhuyu Decoction, such as decocting, filtering, concentrating and drying, were investigated by single factor, and the extraction rate, extract and fingerprint characteristics of the sample were determined after single factor investigation as the key index parameters to determine the best preparation process. Results The preparation process of the substance standard of Shentong Zhuyu Decoction was according to the prescription of Shentong Zhuyu Decoction in Correction of Errors in Medical Classics, Yiqian is 3.73 g, 93.25 g of Shentong Zhuyu Decoction was precisely weighed, 10 times of water was added, soaked for 30 min, boiled by high heat, refluxed for 1 h, filtered with 200 mesh gauze while hot, and added a certain volume of 95% ethanol by volume to 80% alcohol content, after 24 h, the supernatant was collected and lyophilized. Process validation results indicated that the preparation process was stable. Conclusion The preparation process of Shentong Zhuyu Decoction is stable and feasible, which will provide the basis for the study of substance standard of Shentong Zhuyu Decoction and lay the foundation for the study of ancient classic prescription. |
| Key words: classic prescription Shentong Zhuyu Decoction substance standard preparation process Correction of Errors in Medical Classics |
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