| 引用本文: |
张也,伍红年,雷雅婷,刘隆基,王元清,刘乐平,严建业.UPLC-Q-TOF/MS结合镜像对比分析竹节参炮制前后成分变化[J].湖南中医药大学学报,2021,41(11):1689-1697[点击复制] |
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| UPLC-Q-TOF/MS结合镜像对比分析竹节参炮制前后成分变化 |
| 张也,伍红年,雷雅婷,刘隆基,王元清,刘乐平,严建业 |
| (湖南中医药大学科技创新中心, 湖南 长沙 410208;湖南中医药大学科技创新中心, 湖南 长沙 410208;浏阳市人民医院, 湖南 浏阳 410399;湖南中医药大学科技创新中心, 湖南 长沙 410208;湖南中医药大学药学院, 湖南 长沙 4102084;中南林业科技大学生命科学与技术学院, 湖南 长沙 410004) |
| 摘要: |
| 目的 基于超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF/MS）结合镜像技术对比分析竹节参炮制前后成分的变化。方法 采用ACQUITY UPLC BEH C18 （2.1 mm×100 mm，1.7 μm）色谱柱；流动相为0.1%甲酸（A）-乙腈（B），梯度洗脱；质谱采用电喷雾（ESI）离子源，在负离子模式下采集数据。结果 通过对照品指认、软件预测分析，结合文献报道，从竹节参炮制品中鉴定并推测出29个皂苷类化合物，与生品相比新增5种成分。结论 利用UPLC-Q-TOF/MS系统阐明竹节参生品与炮制品中化学成分，并初步归纳其质谱裂解特点，结合镜像对比分析，阐明不同炮制方法对竹节参皂苷类成分的影响，经加热炮制后竹节参中的成分数量与响应面积均发生变化，为竹节参的质量控制和炮制应用研究提供了参考依据。 |
| 关键词: 竹节参 皂苷类 炮制 超高效液相色谱-四极杆飞行时间质谱 |
| DOI：10.3969/j.issn.1674-070X.2021.11.008 |
| 投稿时间：2021-06-26 |
| 基金项目:湖南省自然科学基金项目（2020JJ4470）；湖南省中医药科研计划项目（202072）；湖南中医药大学中药学一流建设学科资助（校行科字〔2018〕3号）。 |
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| Comparative Analysis of Composition Changes of Panacis Japonici Rhizoma Before and After Processing by UPLC-Q-TOF/MS Combined with Mirror Image |
| ZHANG Ye,WU Hongnian,LEI Yating,LIU Longji,WANG Yuanqing,LIU Leping,YAN Jianye |
| (Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The People's Hospital of Liuyang, Liuyang, Hunan 410399, China;Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China) |
| Abstract: |
| Objective To compare and analyze the changes of components of Panacis Japonici Rhizoma before and after processing based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and mirror image technique. Methods The ACQUITY UPLC BEH C18 (2.1 mm×100 mm, 1.7 μm) column was performed; the mobile phase consisted with 0.1% formic acid (A) and acetonitrile (B) for gradient elution; electrospray ion (ESI) source was applied for the analysis under the negative ion mode. Results 29 saponins were identified and inferred from Panacis Japonici Rhizoma processed products by reference substance identification, software prediction analysis and literature reports, and 5 new components were added compared with raw product. Conclusion The chemical constituents of Panacis Japonici Rhizoma raw product and processed products were elucidated by UPLC-Q-TOF/MS system, and the characteristics of mass spectrometry fragmentation were preliminarily summarized. The effects of different processing methods on the saponins of Panacis Japonici Rhizoma were clarified by mirror contrast technique. The number and response area of components in Panacis Japonici Rhizoma changed after heating processing, which provided a reference basis for the quality control and processing of Panacis Japonici Rhizoma. |
| Key words: Panacis Japonici Rhizoma saponins processing ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry |
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