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朱建平,陈俣祯,高萌萌,袁志鹰,谢梦洲.基于UPLC-Q-TOF/MS法对鲜、干百合地黄汤化学成分的比较研究[J].湖南中医药大学学报,2021,41(11):1698-1704[点击复制] |
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| 基于UPLC-Q-TOF/MS法对鲜、干百合地黄汤化学成分的比较研究 |
| 朱建平,陈俣祯,高萌萌,袁志鹰,谢梦洲 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208;中医心肺病证辨证与药膳食疗重点研究室, 湖南 长沙 410208) |
| 摘要: |
| 目的 采用超高效液相色谱-四级杆-飞行时间串联质谱法（UPLC-Q-TOF/MS）对鲜、干百合地黄汤的化学成分进行分析，并对差异成分的抗抑郁机制进行探讨。方法 采用Waters UPLC BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），流动相（0.1%甲酸水溶液-乙腈）梯度洗脱，使用QExactiveFocus质谱仪和Xcalibur软件，基于IDA采集模式获得MS和MS/MS数据进行数据分析。并根据成分保留时间、结合数据库Inhouse MS/MS database及相关文献报道进行结构鉴定。结果 从鲜、干百合地黄汤差异成分中共筛选出35个化合物，其中17个萜类化合物、3个酚类化合物、4个氨基酸类化合物、3个黄酮类化合物、2个生物碱类化合物、6个苯丙素类化合物。鲜品中环烯醚萜类与氨基酸类物质含量明显高于干品，鲜品中生物碱与苯丙素类化合物含量低于干品。结论 UPLC-Q-TOF/MS可快速检测鲜、干百合地黄汤的化学成分，成分多为萜类、氨基酸类和苯丙素类化合物，可为鲜药与干药配方质量控制、解郁药效物质基础研究及其复方制剂开发奠定良好的基础。 |
| 关键词: 鲜百合 干品百合 百合地黄汤 超高效液相色谱-四级杆-飞行时间串联质谱法 差异成分 |
| DOI：10.3969/j.issn.1674-070X.2021.11.009 |
| 投稿时间：2021-06-28 |
| 基金项目:国家自然科技基金项目（81774207）；建生鲜药创研基金资助项目（JSJC-20200106-058，JSYY-20210105-068）；湖南省自然科技基金项目（2019JJ50446，2021JJ40411）；湖南省教育厅资助科研项目（18B241）；湖南中医药大学药食同源工程中心开放基金资助（YSTY08）；中医学一流学科开放基金资助（2018ZYX27）。 |
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| Fresh and Dried Baihe Dihuang Decoction Comparative Study of Chemical Components Based on UPLC-Q-TOF/MS Method |
| ZHU Jianping,CHEN Yuzhen,GAO Mengmeng,YUAN Zhiying,XIE Mengzhou |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Medicine & Food Homologous Functional Food Engineering and Technology Research Center, Changsha, Hunan 410208, China;Key Laboratory of TCM Cardiopulmonary Disease Syndrome Differentiation and Medicine Diet Therapy, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To identify and analyze the chemical constituents of fresh and dried Baihe Dihuang Decoction by ultra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS), and to discuss the anti-depression mechanism of different components. Methods Waters UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm), mobile phase (0.1% formic acid aqueous solution-acetonitrile) was used for gradient elution, QExactiveFocus mass spectrometer and Xcalibur software was used to obtain MS and MS/MS data for data analysis based on IDA acquisition mode. And according to the retention time, combined with the Inhouse MS/MS database and related literature reports for structural identification. Results A total of 35 compounds were screened from the different components of fresh and dry Baihe Dihuang Decoction, including 17 terpenoids, 3 phenols, 4 amino acids, 3 flavonoids, 2 alkaloids and 6 phenylpropanoids. The content of iridoids and amino acids in fresh products was significantly higher than that in dry products. The content of alkaloids and phenylpropanoids in fresh products was lower than that in dry products. Conclusion UPLC-Q-TOF/MS can quickly detect the chemical components in the fresh and dried Baihe Dihuang Decoction. The components are mostly terpenoids, amino acids and phenylpropanoids, which can lay a good foundation for the quality control of fresh and dry medicine formulations, basic research of antidepressant substance and the development of compound preparations. |
| Key words: fresh Baihe (Lilii Bulbus) dried Baihe (Lilii Bulbus) Baihe Dihuang Decoction ultra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry different components |
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