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曹佳男,刘霞,刘涛,刘琼,刘密,常小荣,谢辉.艾灸对腹泻型肠易激综合征大鼠结肠5-HT及5-HT3R的影响[J].湖南中医药大学学报,2021,41(10):1493-1497[点击复制] |
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| 艾灸对腹泻型肠易激综合征大鼠结肠5-HT及5-HT3R的影响 |
| 曹佳男,刘霞,刘涛,刘琼,刘密,常小荣,谢辉 |
| (湖南中医药大学, 湖南 长沙 410208;重庆三峡医药高等专科学校, 重庆 万州 404120;江苏省中西医结合医院, 江苏 南京 210028;郴州市第一人民医院西院, 湖南 郴州 423000) |
| 摘要: |
| 目的 观察艾灸对腹泻型肠易激综合征（diarrhea predominant irritable bowel syndrome，IBS-D）大鼠5羟色胺（5-hydroxytryptamine，5-HT）及5羟色胺3受体（5-hydroxytryptamine 3 receptor，5-HT3R）的影响，分析艾灸治疗IBS-D的作用机制。方法 选取SPF级雄性SD大鼠48只随机分为正常组、模型组、温和灸组、西药组，每组12只。乙酸灌肠后行束缚刺激，每天束缚1 h，持续束缚2周复制IBS-D大鼠模型。造模成功后，温和灸组穴位分为两组：Ⅰ组选肝俞、脾俞、足三里，Ⅱ组选章门、期门、足三里。Ⅰ组、Ⅱ组穴位隔日交替施灸，每次15 min，持续2周。西药组以匹维溴铵片（10 mL/kg）灌胃，1次/d，持续2周。分别在治疗前后观察各组大鼠一般行为学、内脏敏感性、粪便含水量、结肠病理；ELISA法检测结肠组织5-HT的含量；Western blot检测结肠组织5-HT3R的表达。结果 正常组大鼠精神、活动佳，反应灵敏；模型组大鼠精神、活动差、反应迟缓；与模型组相比，温和灸组、西药组大鼠一般行为学表现均有改善。各组大鼠结肠组织形态均正常，无明显充血水肿溃疡、炎症细胞浸润等病理组织学改变。与正常组比较，模型组大鼠内脏敏感性升高（P<0.01）；与模型组相比，温和灸组、西药组大鼠内脏敏感性下降（P<0.01）。与正常组比较，模型组大鼠粪便含水量增加（P<0.01）；与模型组相比，温和灸组、西药组大鼠大便含水量明显降低（P<0.01）。与正常组相比，模型组结肠组织中5-HT、5-HT3R的表达量上升（P<0.05，P<0.01）；与模型组相比，温和灸组、西药组结肠组织中5-HT、5-HT3R的表达量下降（P<0.05，P<0.01）。结论 艾灸可以有效改善IBS-D大鼠腹泻症状和内脏高敏感性，可能与艾灸调节结肠组织中5-HT及其受体的表达相关。 |
| 关键词: 艾灸 肠易激综合征 5羟色胺 5羟色胺3受体 内脏敏感性 |
| DOI：10.3969/j.issn.1674-070X.2021.10.004 |
| 投稿时间：2020-07-23 |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2015CB554502）；国家自然科学基金项目（81874509）；国家中医药管理局全国名老中医药专家传承工作室项目（国中医药人教函〔2019〕41号）；常小荣国家级教学名师工作室建设项目。 |
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| Effect of Moxibustion on Colon 5-HT and 5-HT3R in Diarrhea Predominant Irritable Bowel Syndrome Rats |
| CAO Jia'nan,LIU Xia,LIU Tao,LIU Qiong,LIU Mi,CHANG Xiaorong,XIE Hui |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Chongqing Three Gorges Medical College, Wanzhou, Chongqing 404120, China;Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, Jiangsu 210028, China;West Hospital of Chenzhou First People's Hospital, Chenzhou, Hunan 423000, China) |
| Abstract: |
| Objective To observe the effect of moxibustion on 5-hydroxytryptamine (5-HT) and 5-hydroxytryptamine 3 receptor (5-HT3R) in colon tissue of rats with diarrhea predominant irritable bowel syndrome (IBS-D), and to analyze the mechanism of moxibustion in the treatment of IBS-D. Methods 48 SPF male SD rats were randomly divided into normal group, model group, mild moxibustion group and western medicine group, with 12 rats in each group. After acetic acid enema, the IBS-D rat model was reproduced by restraint stimulation for one hour a day and two weeks. After successful modeling, acupuncture points were divided into two groups in mild moxibustion group:group Ⅰ selected "Ganshu" (BL18), "Pishu" (BL20) and "Zusanli" (ST36), and group Ⅱ selected "Zhangmen" (LR13), "Qimen" (LA14) and "Zusanli" (ST36). Group Ⅰ and group Ⅱ were treated with moxibustion alternately every other day for 15 minutes each time for two weeks. The western medicine group was given piveronium bromide tablets (10 mL/kg), once a day for two weeks. The general behavior, visceral sensitivity, fecal water content and colon pathology were observed before and after treatment. The content of 5-HT in colon tissue was detected by ELISA. The expression of 5-HT3R in colon was detected by Western blot. Results The rats in the normal group had good spirit and activity and sensitive response; the rats in the model group had poor spirit and activity and slow response; compared with the model group, the general behavior of rats in mild moxibustion group and western medicine group were improved. The colonic morphology of rats in each group was normal, without obvious histopathological changes such as congestion, edema, ulcer and inflammatory cell infiltration. Compared with the normal group, the visceral sensitivity of the model group was higher (P<0.01); compared with the model group, the visceral sensitivity of rats in mild moxibustion group and western medicine group decreased (P<0.01). Compared with the normal group, the fecal water content in the model group increased (P<0.01); compared with the model group, the water content of stool in mild moxibustion group and western medicine group decreased significantly (P<0.01). Compared with the normal group, the expression levels of 5-HT and 5-HT3R in the colon of the model group increased (P<0.05, P<0.01); compared with the model group, the expression levels of 5-HT and 5-HT3R in colon tissue of mild moxibustion group and western medicine group decreased (P<0.05, P<0.01). Conclusion Moxibustion can effectively improve diarrhea symptoms and visceral hypersensitivity in IBS-D rats, which may be related to moxibustion regulating the expression of 5-HT and its receptor in colon tissue. |
| Key words: moxibustion irritable bowel syndrome 5-hydroxytryptamine 5-hydroxytryptamine 3 receptor visceral sensitivity |
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