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袁群,胡海林,毛欣,王能,曹淼,何清湖.破壁灵芝孢子粉对大鼠睡眠功能改善效果及机制的研究[J].湖南中医药大学学报,2021,41(7):1037-1041[点击复制] |
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| 破壁灵芝孢子粉对大鼠睡眠功能改善效果及机制的研究 |
| 袁群,胡海林,毛欣,王能,曹淼,何清湖 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南医药学院, 湖南 怀化 418000) |
| 摘要: |
| 目的 观察破壁灵芝孢子粉对正常大鼠睡眠功能的影响，初步探讨其作用机制。方法 将雄性SD大鼠随机分成正常对照组、阳性对照组、破壁灵芝孢子粉低、中、高剂量组（0.42、0.84、1.68 g/kg），每组9只，灌胃剂量为10 mL/（kg·d-1），连续灌胃14 d。末次灌胃后分别进行直接睡眠实验、戊巴比妥钠阈下剂量催眠实验、阈上剂量睡眠潜伏期及睡眠时间实验，并测定大鼠血浆5-羟色胺（5-hydroxytryptamine，5-HT）、多巴胺（dopamine，DA），及下丘脑中5-HT、谷氨酸（glutamic acid，Glu）、γ-氨基丁酸（Gamma-aminobutyric acid，GABA）的含量。结果 破壁灵芝孢子粉能明显缩短大鼠睡眠潜伏期，提高大鼠睡眠率（P<0.05），对睡眠总时长的影响虽无统计学差异，但有延长睡眠时间的趋势。中、高剂量的破壁灵芝孢子粉能够提高大鼠下丘脑5-HT、GABA含量（P<0.05，P<0.01），同时也能提高血浆中5-HT水平、降低DA的含量（P<0.05，P<0.01）。结论 破壁灵芝孢子粉具有睡眠改善作用，其作用机制与调节大鼠脑内5-HT和Glu/GABA平衡，以及血中兴奋-抑制中枢神经系统的神经递质有关。 |
| 关键词: 灵芝孢子粉 睡眠 5-羟色胺 多巴胺 γ-氨基丁酸 |
| DOI：10.3969/j.issn.1674-070X.2021.07.011 |
| 投稿时间：2020-12-18 |
| 基金项目:湖南中医药大学护理学一级学科开放基金项目（2020HLX07）。 |
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| Effect and Mechanisms of the Broken Wall Ganoderma Spore Powder on Sleep Function in Rats |
| YUAN Qun,HU Hailin,MAO Xin,WANG Neng,CAO Miao,HE Qinghu |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Medicine, Huaihua, Hunan 418000, China) |
| Abstract: |
| Objective To observe the effects of broken wall ganoderma spores powder on sleep function in normal rats, and explore the mechanisms. Methods Male SD rats were randomly divided into five groups, normal group, positive group and low, medium and high dose (0.42, 0.84, 1.68 g/kg) broken wall ganoderma spores group, 9 rats in each group, 10 mL/(kg·d) in intragastric administration for 14 days. After the last intragastric administration, direct sleep test, pentobarbital sodium subthreshold dose hypnotism test, supra threshold dose sleep latency test and sleep time test were conducted, and plasma 5-hydroxytryptamine (5-HT), dopamine (DA) and the content of 5-HT, glutamic acid (Glu) and gamma-aminobutyric acid (GABA) in the hypothalamus were measured. Results Broken wall ganoderma spore powder could significantly shorten sleep latency and increase sleep rate (P<0.05). There was no statistical difference in sleep time, but there was a tendency to prolong sleep time. Medium and high doses of broken wall ganoderma spore powder could increase the content of 5-HT and GABA in hypothalamus and decrease the content of Glu (P<0.05, P<0.001), at the same time, the concentration of 5-HT in plasma was increased while the DA were decreased (P<0.05, P<0.001). Conclusion Broken wall ganoderma spores powder can improve sleep, the mechanism of which is related to the regulation of 5-HT and Glu/GABA balance in rat brain, and the neurotransmitter of excitation-inhibition of central nervous system in blood. |
| Key words: broken wall ganoderma spores powder sleep 5-hydroxytryptamine dopamine gamma-aminobutyric acid |
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