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蒋鹏飞,欧晨,彭俊,姚震,田野,杨毅敬,宋厚盼,徐剑,彭清华.枸杞子-丹参对小鼠视网膜Caspase-12前体、Caspase-2表达的影响[J].湖南中医药大学学报,2021,41(4):504-511[点击复制] |
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| 枸杞子-丹参对小鼠视网膜Caspase-12前体、Caspase-2表达的影响 |
| 蒋鹏飞,欧晨,彭俊,姚震,田野,杨毅敬,宋厚盼,徐剑,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;上海市东方医院, 上海 200120) |
| 摘要: |
| 目的 观察枸杞子-丹参对rd10小鼠视网膜Caspase-12前体、Caspase-2表达的影响。方法 将40只rd10小鼠随机分为5组，空白组灌胃生理盐水；对照组灌胃维生素A溶剂；枸杞组灌胃枸杞子中药超微配方颗粒溶液；丹参组灌胃丹参中药超微配方颗粒溶液；枸杞加丹参组（杞参组）灌胃枸杞子加丹参中药超微配方颗粒溶液；8只C57小鼠为野生组，灌胃生理盐水。给药28 d后，RT-qPCR检测小鼠视网膜Caspase-12前体、Caspase-2 mRNA表达情况，免疫组化、免疫荧光法检测小鼠视网膜Caspase-12前体、Caspase-2蛋白表达情况。结果 RT-qPCR检测显示：除杞参组外，各组小鼠视网膜Caspase-12前体、Caspase-2 mRNA相对表达量高于野生组，差异有统计学意义（P<0.05）；杞参组小鼠视网膜Caspase-12前体、Caspase-2 mRNA相对表达量低于空白组、对照组，差异均有统计学意义（P<0.05）。免疫组化检测显示：各组小鼠视网膜Caspase-12前体、Caspase-2平均光密度高于野生组，差异均有统计学意义（P<0.05）；杞参组Caspase-12前体、Caspase-2蛋白平均光密度低于空白组、对照组、枸杞组、丹参组，差异均有统计学意义（P<0.05）。免疫荧光检测显示：各组小鼠视网膜Caspase-12前体、Caspase-2蛋白平均荧光强度高于野生组，差异均有统计学意义（P<0.05）；杞参组Caspase-12前体、Caspase-2蛋白平均荧光强度低于空白组、对照组、枸杞组、丹参组，差异均有统计学意义（P<0.05）。结论 枸杞子-丹参可以抑制rd10小鼠视网膜Caspase-12前体、Caspase-2 mRNA和蛋白的表达，从而抑制感光细胞的凋亡，维持视网膜正常功能结构，保护视功能。 |
| 关键词: 视网膜色素变性 细胞凋亡 枸杞子 丹参Caspase-2 Caspase-12前体 |
| DOI：10.3969/j.issn.1674-070X.2021.04.003 |
| 投稿时间：2021-01-10 |
| 基金项目:国家自然科学基金项目（81804150，82074196）；国家中医药管理局中医眼科学重点学科建设项目（ZK1801YK015）；湖南中医药大学中医学国内一流建设学科；中医药防治五官科疾病湖南省重点实验室建设项目（2017TP1018）；湖南省自然科学基金资助项目（2019JJ40226）；湖南省研究生科研创新项目（CX2018B497）；湖南中医药大学研究生创新课题（2018CX40）。 |
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| Effect of Gouqizi (Lycii Fructus) and Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) on the Expression of Caspase-12 Precursor and Caspase-2 in Retinal of rd10 Mice |
| JIANG Pengfei,OU Chen,PENG Jun,YAO Zhen,TIAN Ye,YANG Yijing,SONG Houpan,XU Jian,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine, Changsha, Hunan 410208, China;First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Shanghai Dongfang Hospital, Shanghai 200120, China) |
| Abstract: |
| Objective To observe the effect of Gouqizi (Lycii Fructus) and Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) on the expression of Caspase-12 precursor and Caspase-2 in rd10 mice. Methods 40 rd10 mice were randomly divided into 5 groups. The blank group was given distilled water; the control group was given intragastric vitamin A solvent; the Gouqizi (Lycii Fructus) group was given intragastric Gouqizi (Lycii Fructus) Chinese medicine ultrafine granular solution; the Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) group was given intragastric Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) Chinese medicine ultrafine formula granule solution; Gouqizi (Lycii Fructus) plus Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) group (QiShen group) was given intragastric Gouqizi (Lycii Fructus) plus Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) Chinese medicine superfine granule solution; 8 C57 mice as the wild group, was given intragastric distilled water. 28 days after the administration, RT-qPCR was used to detect the expression of Caspase-12 precursor and Caspase-2 mRNA in the retina of rd10 mice, and the expression of Caspase-12 precursor and Caspase-2 proteins in the retina was detected by immunohistochemistry and immunofluor-escence. Results The RT-qPCR test showed that except for the QiShen group, the relative expression levels of Caspase-12 precursor and Caspase-2 mRNA in the retina of each group were higher than that of the wild group, and the differences were statistically significant (P<0.05); the relative expression levels of Caspase-12 precursor and Caspase-2 mRNA in the retina of the QiShen group were lower than that of the blank group and the control group, the differences were statistically significant (P<0.05). Immunohistochemical detection showed that the average optical density of Caspase-12 precursor and Caspase-2 in the retina of mice in each group was higher than that of the wild group, and the differences were statistically significant (P<0.05); the average optical density of Caspase-12 precursor and Caspase-2 of the QiShen group was lower than that of the blank group, control group, Gouqizi (Lycii Fructus) group, and Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) group, and the differences were statistically significant (P<0.05). Immunofluorescence detection showed that the average fluorescence intensity of Caspase-12 precursor and Caspase-2 in the retina of mice in each group was higher than that in the wild group, and the differences were statistically significant (P<0.05); Caspase-12 precursor and Caspase-2 average fluorescence intensity of QiShen group was lower than that of the blank group, control group, Gouqizi (Lycii Fructus) group and Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) group, and the differences were statistically significant (P<0.05). Conclusion Gouqizi (Lycii Fructus) and Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) can inhibit the expression of rd10 mice Caspase-12 precursors, Caspase-2, thereby inhibiting the apoptosis of photoreceptor cells, maintaining normal functional structure of retina, protecting view function. |
| Key words: retinitis pigmentosa apoptosis Gouqizi (Lycii Fructus) Danshen (Salviae Miltiorrhizae Radix Et Rhizoma) Caspase-2 Caspase-12 precursor |
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