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范婧莹,娄余,张娜,彭瑶,朱莹.三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响[J].湖南中医药大学学报,2019,39(8):937-942[点击复制] |
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| 三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响 |
| 范婧莹,娄余,张娜,彭瑶,朱莹 |
| (湖南中医药大学中西医结合学院, 湖南 长沙 410208;湖南中医药大学研究生院, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响。方法 将实验分成6组：空白对照组、LV-RNAi 组、Survivin-RNAi-LV组、空白对照+中药组、LV-RNAi+中药组、Survivin-RNAi-LV+中药组；采用流式细胞仪检测各组细胞的凋亡，划痕实验检测各组细胞的迁移能力。结果 细胞凋亡率：Survivin-RNAi-LV组明显高于空白对照组和LV-RNAi组（P<0.05）；Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较明显较高（P<0.05）；Survivin-RNAi-LV+中药组高于Survivin-RNAi-LV组，差异具有统计学意义（P<0.05）。胃癌细胞的24 h迁移率：Survivin-RNAi-LV组明显低于空白对照组和LV-RNAi组（P<0.05）；Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较，其结果明显低于后两组（P<0.05）；Survivin-RNAi -LV+中药组与Survivin-RNAi-LV组比较，细胞迁移率明显降低，差异有统计学意义（P<0.05）。结论 Survivin基因沉默转染，增强了三物白散诱导胃癌SGC-7901细胞凋亡和抑制其迁移的作用。 |
| 关键词: Survivin 三物白散 胃癌 细胞凋亡 迁移 |
| DOI：10.3969/j.issn.1674-070X.2019.08.002 |
| 投稿时间：2018-09-04 |
| 基金项目:国家自然科学基金资助项目（81503561）；湖南省教育厅科研基金项目（16C1212）。 |
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| Effects of Sanwubai Powder on Apoptosis and Migration of Gastric Cancer SGC-7901 Cells Transfected with Survivin-RNA Gene Silencing |
| FAN Jingying,LOU Yu,ZHANG Na,PENG Yao,ZHU Ying |
| (College of Integrated Chinese and Western Medicines, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effects of Sanwubai Powder on apoptosis and migration of gastric cancer SGC-7901 cells transfected with Survivin-RNA gene silencing. Methods The experiment was divided into 6 groups:a blank control group, a LV-RNAi group, a Survivin-RNAi-LV group, a blank control group + TCM group, a LV-RNAi + TCM group, a Survivin-RNAi -LV + TCM group. The cell apoptosis was observed by flow cytometry, and the cell migration was assessed by cell scratch experiment. Results The apoptotic rate of the Survivin-RNAi -LV group was significantly higher than that of blank control group and LV-RNAi group (P<0.05); The apoptosis rate of the Survivin-RNAi-LV + TCM group was significantly higher than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05); The Survivin-RNAi-LV+ TCM group was higher than the Survivin-RNAi-LV group, and the difference was statistically significant (P<0.05). The 24 hour cell migration rate in the Survivin-RNAi -LV group was significantly lower than that in the blank control group and the LV-RNAi group (P<0.05). The Survivin-RNAi -LV + TCM group was significantly lower than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05); Compared with the Survivin-RNAi-LV group, the cell migration rate of the Survivin-RNAi-LV + TCM group was significantly decreased, and the difference was statistically significant (P<0.05). Conclusion After Survivin gene silencing transfection, the sensitivity of SGC-7901 cells to Sanwubai Powder was significantly enhanced, which further promoted the apoptosis of SGC-7901 cells and inhibited their migration. |
| Key words: Survivin Sanwubai Powder gastric cancer apoptosis migration |
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