| 引用本文: |
李海霞,张勇华,任晓丹,孟欣茹,周娟娟,王轻轻,何芳,罗银河.建立RSV联合OVA诱发幼年大鼠哮喘模型的方法[J].湖南中医药大学学报,2018,38(12):1371-1373[点击复制] |
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| 建立RSV联合OVA诱发幼年大鼠哮喘模型的方法 |
| 李海霞,张勇华,任晓丹,孟欣茹,周娟娟,王轻轻,何芳,罗银河 |
| (河北省中医院, 河北 石家庄 050011;湖南省儿童医院, 湖南 长沙 410006;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨呼吸道合胞病毒（respiratory syncytial virus，RSV）滴鼻联合卵清白蛋白（ovalbumin，OVA）雾化方法，建立大鼠哮喘模型的可行性。方法 将SD大鼠共30只随机分为正常对照组（A组）、哮喘模型组（B组）和地塞米松组（C组）3组，每组10只，分别给予相应干预，两周后观察大鼠反应，应用肺功能测量仪测定气道阻力（Re值）、支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中细胞计数与分类，并观察肺组织病理改变。结果 A组大鼠反应及Re值基本正常，B组大鼠出现喘息表现，且测Re值明显高于A组（P<0.05），C组大鼠较B组有所减轻；与A组比较，B组、C组BALF中白细胞计数显著升高（P<0.01），以嗜酸性粒细胞比例为主（P<0.01），C组较B组BALF中白细胞计数、嗜酸性粒细胞比例显著下降（P<0.01）；肺组织病理A组支气管肺泡结构基本正常，B组可见支气管结构紊乱、管壁增厚以及炎性细胞浸润等，C组较B组有所减轻。结论 RSV联合OVA诱发方法，能够成功建立幼年大鼠哮喘模型，为进一步实验研究奠定基础。 |
| 关键词: 哮喘 动物模型 联合诱发 呼吸道合胞病毒 卵清蛋白 |
| DOI：10.3969/j.issn.1674-070X.2018.12.004 |
| 投稿时间：2018-01-24 |
| 基金项目:河北省重点研发计划项目（17277713D）；湖南省自然科学基金项目（2015JJ2016）。 |
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| A Method for Establishing Young Rat Model of Asthma Induced by Respiratory Syncytial Virus Combined with Ovalbumin |
| LI Haixia,ZHANG Yonghua,REN Xiaodan,MENG Xinru,ZHOU Juanjuan,WANG Qingqing,HE Fang,LUO Yinhe |
| (Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei 050000, China;Hunan Children's Hospital, Changsha, Hunan 410006, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the feasibility of intranasal delivery of respiratory syncytial virus (RSV) with ovalbumin (OVA) atomization to establish a rat model of asthma.Methods A total of 30 Sprague-Dawley rats were equally and randomly divided into group A (normal control group), group B (asthma model group), and group C (dexamethasone group) to receive respective treatments. After two weeks, the reaction of rats was observed. Airway resistance (Re value) was determined using a pulmonary function meter. Cell counting and classification were performed for bronchoalveolar lavage fluid (BALF). The pathological changes of lung tissue were evaluated.Results Group A exhibited basically normal reaction and Re value, and group B showed wheezing and a significantly higher Re value than group A (P<0.05); group C showed improvements than group B. Compared with group A, group B and group C had significantly increased leukocyte count in BALF (P<0.01), mainly dominated by eosinophils (P<0.01). Compared with group B, group C exhibited significantly reduced leukocyte count and eosinophil percentage in BALF (P<0.01). Pathology of lung tissue revealed that group A had basically normal bronchoalveolar structure, group B showed disorder of bronchial structure, bronchial wall thickening, and inflammatory cell infiltration, and group C showed alleviation compared with group B.Conclusion The young rat model of asthma can be established by RSV combined with OVA, which lays a foundation for further experimental study. |
| Key words: asthma animal model jointly induced respiratory syncytial virus ovalbumin |
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