| 引用本文: |
王海兰,周湘乐,谭婷,肖碧跃.百合地黄汤对抑郁症大鼠血清IL-10和海马DA的影响[J].湖南中医药大学学报,2018,38(11):1326-1330[点击复制] |
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| 百合地黄汤对抑郁症大鼠血清IL-10和海马DA的影响 |
| 王海兰,周湘乐,谭婷,肖碧跃 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨百合地黄汤对慢性应激性抑郁模型大鼠血清抗炎因子白介素-10（interleukin-10，IL-10）和海马神经递质多巴胺（dopamine，DA）表达水平的影响，为其治疗抑郁症提供依据。方法 将50只雌性成年SD大鼠随机分为空白对照组，模型组，百合地黄汤低、高剂量组，氟西汀组等5组，每组10只，采用慢性应激刺激和独立隔离喂养相结合的方法建立抑郁大鼠模型，模型组与空白对照组正常饲养，其余组在造模的基础上给予相应干预。造模28 d以旷场实验、强迫游泳实验进行行为学检测，ELISA法检测各组大鼠血清抗炎因子IL-10的水平，免疫组化法检测大鼠海马神经递质DA的表达。结果 与空白对照组对比，模型组旷场实验总分降低，强迫游泳不动时间延长，血清抗炎因子IL-10和海马神经递质DA含量明显下降（P<0.01）；与模型组对比，百合地黄汤低剂量组、高剂量组旷场实验总分升高，强迫游泳不动时间缩短，血清抗炎因子IL-10和海马神经递质DA含量显著增加（P<0.01）。结论 百合地黄汤抗抑郁症模型大鼠的抑郁状态，可能与其能够有效干预血清中抗炎因子IL-10和海马神经递质DA有关。 |
| 关键词: 百合地黄汤 白介素-10 多巴胺 抑郁症 |
| DOI：10.3969/j.issn.1674-070X.2018.11.024 |
| 投稿时间：2018-01-03 |
| 基金项目:湖南省科技厅项目（2015JC3077） |
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| Effect of Baihe Dihuang Decoction on Serum Anti-inflammatory Factor Inerleukin-10 and Hippocampal Neurotransmitter Dopamine in Rats with Depression |
| WANG Hailan,ZHOU Xiangle,TAN Ting,XIAO Biyue |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effect of Baihe Dihuang Decoction on serum anti-inflammatory factor interleukin-10 (IL-10) and hippocampal neurotransmitter dopamine (DA) in rats with depression induced by chronic stress, and to provide a basis for the role of Baihe Dihuang Decoction in the treatment of depression. Methods A total of 50 adult female Sprague-Dawley rats were randomly divided into blank control group, model group, low-and high-dose Baihe Dihuang Decoction groups, and fluoxetine group, with 10 rats in each group. A rat model of depression was established by chronic stress stimulation and isolated feeding. After the model was established, the rats in the model group and the blank control group were given normal feeding, and those in the other groups were given corresponding intervention. On day 28 after modeling, the open field test and forced swimming test were used for behavioral assessment, ELISA was used to measure the serum level of IL-10, and immunohistochemistry was used to measure the expression of DA in the hippocampus. Results Compared with the blank control group, the model group had a significant reduction in the total score of open field test, a significant increase in immobility time in forced swimming test, and significant reductions in serum IL-10 and hippocampal DA (P<0.01). Compared with the model group, the low-and high-dose Baihe Dihuang Decoction groups had a significant increase in the total score of open field test, a significant reduction in immobility time in forced swimming test, and significant increases in serum IL-10 and hippocampal DA (P<0.01). Conclusion Baihe Dihuang Decoction exerts a therapeutic effect on depressive state in rats with depression, possibly by effectively regulating serum IL-10 and hippocampal DA. |
| Key words: Baihe Dihuang Decoction interleukin-10 dopamine depression |
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