| 引用本文: |
王园园,张国山,李菲,刘霞,刘密,郁洁.针刺预处理对急性胃黏膜损伤大鼠EGF、TGF-α及TFF3含量的影响[J].湖南中医药大学学报,2018,38(11):1253-1257[点击复制] |
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| 针刺预处理对急性胃黏膜损伤大鼠EGF、TGF-α及TFF3含量的影响 |
| 王园园,张国山,李菲,刘霞,刘密,郁洁 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察针刺预处理中脘、足三里穴对急性胃黏膜损伤大鼠胃组织中表皮生长因子（EGF）、转化生长因子-α（TGF-α）及胃黏膜中三叶因子家族-3（TFF3）的影响。方法 将32只健康SD大鼠随机分为4组，即空白组、模型组、针刺组、针刺非穴组。针刺预处理8 d采用无水乙醇灌胃的方法制备急性胃黏膜损伤模型。光镜下观察大鼠胃黏膜病理组织形态变化，免疫组化法测定胃黏膜组织中EGF、TGF-α及TFF3的含量。结果 与空白组相比，模型组大鼠中胃黏膜损伤指数、EGF、TGF-α及TFF3的表达增强（P<0.01）；与模型组、针刺非穴组相比，针刺穴位组TGF-α、EGF的表达升高（P<0.01），而TFF3、胃黏膜损伤指数降低（P<0.01）。结论 针刺足三里、中脘穴能够降低无水乙醇对胃黏膜的损伤指数，可通过调节EGF、TGF-α及TFF3的含量，来提高胃黏膜损伤修复的程度，且针刺穴位组对胃黏膜损伤的保护作用具有穴位特异性。 |
| 关键词: 急性胃黏膜损伤 针刺预处理 中脘 足三里 胃黏膜保护因子 |
| DOI：10.3969/j.issn.1674-070X.2018.11.007 |
| 投稿时间：2018-08-10 |
| 基金项目:国家自然科学基金项目（81603705，81774438）；湖南省自然科学基金项目（2017JJ3245）；湖南省中医药科研计划项目重点课题（201911） |
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| Effect of Acupuncture Pretreatment on Levels of EGF, TGF-α, and TFF3 in Rats with Acute Gastric Mucosal Injury |
| WANG Yuanyuan,ZHANG Guoshan,LI Fei,LIU Xia,LIU Mi,YU Jie |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of acupuncture pretreatment at Zhongwan and Zusanli points on the epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-α) in gastric tissue and the trefoil factor 3 (TFF3) in gastric mucosa in rats with acute gastric mucosal injury. Methods Thirty-two healthy Sprague-Dawley rats were randomly divided into four groups:blank group, model group, acupuncture group, and non-acupoint acupuncture group. After 8 days of acupuncture pretreatment, an acute gastric mucosal injury model was prepared by intragastric administration of absolute ethanol. The pathological changes of gastric mucosa in rats were evaluated by optical microscopy. The levels of EGF, TGF-α, and TFF3 in gastric mucosa were measured by immunohistochemistry. Results Compared with the blank group, the model group had significantly increased gastric mucosal injury index and expression levels of EGF, TGF-α, and TFF3 (P<0.01). Compared with the model group and non-acupoint acupuncture group, the acupoint acupuncture group had significantly higher expression levels of TGF-α and EGF (P<0.01), but significantly lower level of TFF3 and gastric mucosal injury index (P<0.01). Conclusion Acupuncture at Zusanli and Zhongwan points can reduce the injury index of gastric mucosa caused by absolute ethanol. It can promote the recovery from gastric mucosal injury by regulating the levels of EGF, TGF-α, and TFF3. The protective effect of acupuncture against gastric mucosal injury has acupoint specificity. |
| Key words: acute gastric mucosal injury acupuncture pretreatment Zhongwan Zusanli gastric mucosal protective factor |
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