| 引用本文: |
肖小芹,汤婉婷,成绍武,邓桂明.小鼠阴道涂片技术及其染色方法的研究[J].湖南中医药大学学报,2018,38(4):376-378[点击复制] |
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| 小鼠阴道涂片技术及其染色方法的研究 |
| 肖小芹,汤婉婷,成绍武,邓桂明 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 比较研究棉签法、生理盐水冲洗法进行阴道涂片，HE染色法、瑞氏染色法、巴氏染色法染色对C57BL/6小鼠阴道涂片细胞组织染色的效果，为小鼠动情周期的确定寻找便捷、有效的检验方法。方法 对10只C57BL/6雌性小鼠分两笼饲养，每日10：00用棉签法、生理盐水法进行阴道涂片，连续15 d，并使用HE染色法、瑞氏染色法、巴氏染色法，观察记录。结果 连续实验生理盐水法优于棉签法，HE染色染料易于获得；瑞氏染色分型清楚，染色佳；巴氏染色形态清晰，分色明显，透明度好。结论 大样本量旨在判断动情周期的发生与改变实验应采用瑞氏染色法，可辅以巴氏染色以增强实验动物数据资料的可靠性。 |
| 关键词: C57BL/6 动情周期 阴道涂片 HE染色 瑞氏染色 巴氏染色 |
| DOI：10.3969/j.issn.1674-070X.2018.04.003 |
| 投稿时间：2018-01-07 |
| 基金项目:湖南省中医科研计划重点项目（201815）；湖南省教育厅项目（17C1219）。 |
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| Study of Vaginal Smear and Staining Methods of Mice |
| XIAO Xiaoqin,TANG Wanting,CHENG Shaowu,DENG Guiming |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To compare the staining effect of HE staining, Wright staining and Pap staining on the cell smear of C57BL/6 mice, and to find a relatively simple and effective staining method for identification of estrous cycle. Methods The 10 female C57BL/6 mice were taken vaginal smear at 10:00 every morning, with the course of 15 days. The results were analyzed by HE staining, Wright staining and Papanicolaou stainining methods. Results The normal saline method was better than swab method. HE staining method was simple and easy. Wright staining method was clear with obvious color separation. Papanicolaou stainining was with good diaphaneity. Conclusion The Wright staining method is firstly selected in large sample examination of mice vaginal smear for estimating the development of estrous cycle. The reliability of experimental information can be supplemented by Papanicolaou stainining method. |
| Key words: C57BL/6 estrous cycle vaginal smear HE staining Wright staining Papanicolaou stainining |
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