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朱洁,王叶,郭璇,刘乐平,韩志飞.新型造模法制备功能性消化不良肝郁脾虚证大鼠模型[J].湖南中医药大学学报,2018,38(4):372-375[点击复制] |
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| 新型造模法制备功能性消化不良肝郁脾虚证大鼠模型 |
| 朱洁,王叶,郭璇,刘乐平,韩志飞 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 从病因胃排空延缓、内脏高敏性、心理因素等入手改良建立新的功能性消化不良肝郁脾虚证动物模型。方法 将SD大鼠随机分为5组：高脂组（A组）、大黄组（B组）、复合组（C组）、夹尾对照组（D组）、空白对照组（E组）。除空白对照组，其余4组以束缚和游泳为基础，在A组加入可以导致胃排空延缓的高脂，在B组加入可以导致脾虚的苦寒泻下药大黄煎剂，并将A、B进行结合形成复合组（C组），而D组是国内常用的造模方法即夹尾刺激法，且以D组作为对照，通过观察行为学状态，检测胃排空率、小肠推进率对造模结果进行评价。结果 造模结束后，A、B、C、D组大鼠均出现扎堆、靠边，活跃度降低，攻击性减少，易受惊吓且易怒，毛发枯乱无光泽，粪便变软等表现；与E组相比，其他4组造模组的胃排空率和小肠推进率均有降低，C、D组降低显著，差异具有统计学意义（P<0.01）。结论 选用复合组的造模方法可以使大鼠出现一系列的肝郁脾虚症状，并且有效地降低大鼠的胃排空率和小肠推进率。 |
| 关键词: 功能性消化不良 肝郁脾虚证 动物模型 |
| DOI：10.3969/j.issn.1674-070X.2018.04.002 |
| 投稿时间：2016-11-16 |
| 基金项目:国家自然科学基金项目（81403384）；湖南省教育厅优秀青年项目（15B181）；湖南中医药大学校级创新性实验课题（30）。 |
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| A New Method for Building the Model of Functional Dyspepsia Rats with Liver Depression and Spleen Deficiency Syndrome |
| ZHU Jie,WANG Ye,GUO Xuan,LIU Leping,HAN Zhifei |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective From the delay of gastric emptying, visceral hypersensitivity, psychological factors and other causes to improve the establishment of a new animal model of dyspepsia rats with liver depression and spleen deficiency syndrome. Methods The SD rats were randomly divided into five groups:high fat group (group A), rhubarb group (group B), combination group (group C), clip tail control group (group D), blank control group (group E). Except for the blank control group (group E), the remaining four groups were given the basis training of restraint and swimming. In group A, the high fat caused delay of gastric emptying was added. In group B, bitter cold diarrhea decoction caused spleen deficiency was added, and group C was formed by combination of group A and group B. Group D as a control group was given a commonly used modeling method of tailgating stimulation. The modeling results were evaluated by observing behavioral status, gastric emptying rate, small intestine propulsion rate. Results After modeling, rats in group A, B, C and D appeared the behavior of getting together, keeping to the side, decreased activity, reduced aggression, vulnerable to scared and irritable, hair dull and dull, and soft stool. Compared with group E, the gastric empting rate and small intestine advance rate in other four groups decreased, and the decrease degree in group C and D was more obvious, the differences were statistically significant (P<0.01). Conclusion The compounding method could make rats appear a series of symptoms of liver depression and spleen deficiency, and effectively reduce the gastric emptying rate and intestinal propulsion rate in rats. |
| Key words: functional dyspepsia liver depression and spleen deficiency animal models |
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