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史彦飞,谭枚秀,徐佳,刘奇英,廖杏.穿破石对SOI模型大鼠NF-κB、MCP-1表达的影响[J].湖南中医药大学学报,2017,37(9):939-942[点击复制] |
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| 穿破石对SOI模型大鼠NF-κB、MCP-1表达的影响 |
| 史彦飞,谭枚秀,徐佳,刘奇英,廖杏 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 研究穿破石对输卵管炎性阻塞性不孕症（salpingitc obstructive infertility，SOI）模型大鼠核转录因子Kappa B（nuclear transcription factor Kappa B，NF-κB）、单核细胞趋化蛋白-1（monocyte chemotactic protein-1，MCP-1）表达的影响。方法 采用金黄色葡萄球菌、大肠杆菌和解脲支原体混合菌液接种法建立SOI大鼠模型。造模成功后，随机分成穿破石低、中、高剂量组，妇炎康软胶囊组，假手术组，模型组和空白组7组。予相应药物治疗30 d后，处死大鼠，取各组输卵管组织观察病理学改变，用免疫组化法检测各组大鼠输卵管组织中NF-κB、MCP-1的表达。结果 肉眼观察，与模型组对比，穿破石低、中、高剂量组输卵管肿胀、充血现象均有不同程度减轻，其中，穿破石高剂量组输卵管结构较清晰。光镜下，穿破石低、中、高剂量均不同程度地改善大鼠输卵管组织炎症反应，促进其病变上皮细胞增生修复，减轻内膜充血水肿。与正常组比较，模型组输卵管组织中NF-κB、MCP-1表达明显升高（P<0.01）。与模型组比较，穿破石高、中、低剂量组输卵管组织中NF-κB、MCP-1表达均明显降低（P<0.01）。结论 穿破石可促进受损组织与细胞的形态结构及其生理功能的恢复，其作用机制可能与抑制NF-κB的异常活化，从而抑制MCP-1的转录合成有关。 |
| 关键词: 穿破石 输卵管炎性阻塞性不孕 核转录因子Kappa B 单核细胞趋化蛋白-1 |
| DOI：10.3969/j.issn.1674-070X.2017.09.005 |
| 投稿时间：2017-02-21 |
| 基金项目:湖南省中医药管理局课题（2015115）。 |
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| Effects of Chuanposhi on the Expression of NF-κB and MCP-1 in SOI Model Rats |
| SHI Yanfei,TAN Meixiu,XU Jia,LIU Qiying,LIAO Xing |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To study the effect of Chuanposhi on NF-κB and MCP-1 level in rats with salpingitc obstructive infertility (SOI). Methods The SOI rat models were established by mixed bacteria suspension inoculation method of Staphyloccocus aureus, Escherichia coli and Ureaplasma urealyticum. The successful model rats were randomly divided into 7 groups including high, middle and low dosage of Chuanposhi groups, Fuyankang soft capsule group, sham-operation group, blank group. The model rats were killed after treatment with corresponding medicines for 30 days. Pathologic changes of fallopian tube were measured by a microscope. The NF-κB and MCP-1 levels in SOI model rats were measured by immunohistochemical method. Results Visual inspection: the tubal swelling and hyperemia in high, medium and low dosage of Chuanposhi groups reduced in different degrees compared with those in the model group, and the structures in high dosage of Chuanposhi group were clear. Light microscopic inspection: the low, medium and high dosage of Chuanposhi could improve the oviduct tissue inflammation of rats in different degrees, promote its lesion repair of epithelial cell proliferation, alleviate congestion and edema. The expressions of NF-κB and MCP-1 in model group were markedly higher than those in normal control group (P<0.01)． The expressions of NF-κB and MCP-1 in high, medium, low dosages of Chuanposhi groups model group were significantly lower than those in the model group (P<0.01)． Conclusion Chuanposhi could promote the recovery of morphology and physiological function of damaged tissues and cells. Its mechanism may be associated with inhibiting the NF-κB abnormal activation and preventing the transcription of MCP-1 synthesis． |
| Key words: Chuanposhi salpingitc obstructive infertility nuclear transcription factor Kappa B monocyte chemotactic protein-1 |
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