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张博,叶阳,刘学伟,张雨薇,黄树明.电针改善去势雌性大鼠记忆障碍的机制研究[J].湖南中医药大学学报,2017,37(6):670-673[点击复制] |
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| 电针改善去势雌性大鼠记忆障碍的机制研究 |
| 张博,叶阳,刘学伟,张雨薇,黄树明 |
| (黑龙江中医药大学中医药研究院, 黑龙江 哈尔滨 150040;齐齐哈尔医学院, 黑龙江 齐齐哈尔 161006;黑龙江中医药大学, 黑龙江 哈尔滨 150040) |
| 摘要: |
| 目的 观察电针对去势雌性大鼠记忆、脑内脑源性神经营养因子（BDNF）及其受体酪氨酸激酶受体B（TrkB）表达的影响。方法 将SD大鼠36只随机分为假手术组，模型组和电针组，每组12只。假手术组大鼠仅切开皮肤，其余各组大鼠给予卵巢摘除，15 d后，电针组给予电针刺激，隔日1次，持续45 d。Morris水迷宫测试各组大鼠学习记忆能力，免疫组化法检测各组大鼠海马CA1区BDNF和TrkB蛋白的表达。结果 水迷宫结果显示，与假手术组比较，模型组大鼠的逃避潜伏期明显延长（P<0.05），穿越平台次数明显减少（P<0.01）。与模型组比较，电针组大鼠的逃避潜伏期明显缩短（P<0.05），穿越平台次数明显增加（P<0.01）。免疫组化结果显示，与假手术组相比，模型组大鼠海马CA1区BDNF表达明显减少（P<0.01）。与模型组相比，电针组大鼠海马CA1区BDNF表达明显增加（P<0.01）。但各组TrkB表达无显著差异（P>0.05）。结论 电针改善去势雌性大鼠记忆障碍的机制可能与增加海马CA1区BDNF的表达有关。 |
| 关键词: 电针 去势大鼠 记忆障碍 脑源性神经营养因子 |
| DOI：10.3969/j.issn.1674-070X.2017.06.025 |
| 投稿时间：2017-01-22 |
| 基金项目:国家自然科学基金青年科学基金项目（81603321）；黑龙江省博士后资助项目（LBH-Z13200）；黑龙江省自然科学基金（H2016072）；黑龙江中医药大学科研基金项目（2015bs04）。 |
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| Electroacupuncture Improves Memory Dysfunction in Ovariectomized Rats |
| ZHANG Bo,YE Yang,LIU Xuewei,ZHANG Yuwei,HUANG Shuming |
| (Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China;Qiqihar Medical University, Qiqihar, Heilongjiang 161006) |
| Abstract: |
| Objective To observe the effects of electroacupuncture on memory and the expression of brain-derived neurotrophic factor (BDNF) and its receptor tropomyosin receptor kinase B (TrkB) in ovariectomized rats hippocampus. Methods The 36 SD rats were randomly divided into sham-operation group, model group and EA group, 12 rats in each group. Rats in sham-operation group were incised skin, while other two groups rats were ovariectomized. 15 days after ovariectomy, EA group were treated with electroacupuncture every other day for 45 days. Then memory function was evaluated by Morris water maze test and expression of BDNF and TrkB in hippocampal CA1 area were determined by immunohistochemical staining. Results The results showed that the escape latency prolonged significantly in model group (P<0.05) and times of passing through the platform decreased significantly (P<0.01), compared with that of sham group. And both of escape latency and times of passing through the platform were reversed in EA group, compared with that of model group. The immunohistochemical staining showed that expression of BDNF in hippocampal CA1 declined significantly in model group (P<0.01), compared with that of sham group, while this expression in EA group was increased significantly versus model group (P<0.01). However, expression of TrkB in each group didn't show any significance (P>0.05). Conclusion Electroacupuncture could improve memory dysfunction induced by ovariectomy partly due to the increase of expression of BDNF in hippocampal CA1. |
| Key words: electroacupuncture ovariectomized rats memory dysfunction brain-derived neurotrophic factor |
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