| 引用本文: |
唐学,肖靓宜,吴清明,钟茜云,陈翼,何可旺,胡晓妹,伍果美.电针颈夹脊穴对颈型颈椎病模型兔椎间盘软骨细胞MMP-3、TGF-β1的影响[J].湖南中医药大学学报,2017,37(6):674-678[点击复制] |
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| 电针颈夹脊穴对颈型颈椎病模型兔椎间盘软骨细胞MMP-3、TGF-β1的影响 |
| 唐学,肖靓宜,吴清明,钟茜云,陈翼,何可旺,胡晓妹,伍果美 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 采用ELISA法观察电针颈型颈椎病（CS）模型兔对其椎间盘软骨中基质金属蛋白酶-3（MMP-3）、转化生长因子-β1（TGF-β1）含量的影响，从而探讨电针治疗颈型CS可能的作用机制。方法 将18只新西兰纯种兔按随机数字表法随机分为空白组、模型组、治疗组，每组6只，除空白组外其余两组采用复合病因造模法造模，连续10周。造模成功后，治疗组予电针治疗，其他两组正常饲养，连续4周。耳缘静脉注射空气栓塞处死各组动物，取C4-7椎间盘组织，分离出软骨终板，采用ELISA法观察椎间盘软骨中MMP-3、TGF-β1的含量。结果 与空白组比较，模型组兔椎间盘软骨细胞中MMP-3、TGF-β1含量均升高，差异具有显著统计学意义 （P<0.01）。与模型组比较，治疗组兔椎间盘软骨细胞中MMP-3含量降低，TGF-β1含量升高，差异具有显著统计学意义（P<0.01）。结论 电针颈型CS模型兔颈夹脊穴可以降低其椎间盘软骨细胞中MMP-3含量，抑制其对软骨细胞外基质（ECM）的降解，维持血窦-软骨终板界面弥散营养物质的能力，延缓或阻止椎间盘退变的发生；同时可以升高TGF-β1含量，促进ECM的合成，对椎间盘进行修复，维持椎间盘软骨终板的正常形态结构，减轻早期椎间盘退变的程度。 |
| 关键词: 电针 颈夹脊穴 颈型颈椎病 软骨细胞外基质 基质金属蛋白酶3 转化生长因子-β1 |
| DOI：10.3969/j.issn.1674-070X.2017.06.026 |
| 投稿时间：2016-09-30 |
| 基金项目:湖南省高校创新平台开放基金资助项目(15K096)。 |
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| Effects of Electro-acupuncture at Cervical Jiaji Points on MMP-3 and TGF-β1 in Intervertebral Disc Chondrocytes of Cervical Spondylotic Model Rabbits |
| TANG Xue,XIAO Liangyi,WU Qingming,ZHONG Qianyun,CHEN Yi,HE Kewang,HU Xiaomei,WU Guomei |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effect of electro-acupuncture on the expression of MMP-3 and TGF-β1 in cervical intervertebral disc cartilage in cervical spondylotic (CS) model rabbits, and to explore the possible mechanism of EA treatment. Methods The eighteen New Zealand pure rabbits were randomly divided into blank group, model group and treatment group, six rabbites in each group. Except for the blank group, the other two groups were made by compound etiological modeling method for 10 weeks. After modeling successfully, the treatment group was given electro-acupuncture treatment, the other two groups were given normal feeding, for 4 weeks. The animals in each group were killed by air embolisn complicating injection. The C4-7 intervertebral disc tissue was isolated and the cartilage endplate was isolated. The contents of MMP-3 and TGF-β1 in intervertebral disc cartilage were detected by ELISA. Results Compared with the blank group, the contents of MMP-3 and TGF-β1 in the intervertebral disc chondrocytes of the model group significantly increased (P<0.01). Compared with the model group, the content of MMP-3 and TGF-β1 in the intervertebral disc chondrocytes of the treatment group significantly decreased (P<0.01). Conclusion Electro-acupuncture cervical cervicogenic model could decrease the content of MMP-3 in intervertebral disc chondrocytes and inhibit the degradation of ECM in chondrocytes, delay or prevent the diffusion of nutrients in cartilage endplate interface. It also could increase the level of TGF-β1, promote the synthesis of ECM, repair the intervertebral disc, maintain the normal form of cartilage endplate structure, reduce the extent of early disc degeneration. |
| Key words: electro-acupuncture cervical Jiaji points neck cervical spondylosis cartilage extracellular matrix matrix metalloproteinase-3 transforming growth factor-β1 |
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