| 引用本文: |
王华1，蔡萍2，万丹2.双黄花片HPLC 指纹图谱的研究及
其绿原酸和黄芩苷含量测定[J].湖南中医药大学学报,2012,32(7):41-44[点击复制] |
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| 双黄花片HPLC 指纹图谱的研究及
其绿原酸和黄芩苷含量测定 |
| 王华1，蔡萍2，万丹2 |
| (1.湖南中医药大学第一附属医院 2.湖南省中医药研究院超微工程中心) |
| 摘要: |
| 目的研究双黄花片的HPLC 指纹图谱和其主要成分绿原酸与黄芩苷的含量。方法采用HPLC 测定，色
谱柱为Inertsil ODS-2 C18(250 mm×4.6 mm， 5 μm)；乙腈-0.2％磷酸溶液为流动相，梯度洗脱；检测波长为280 nm；
流速为1.0 mL/min；柱温为25 ℃。结果确定了10 个共有峰，10 批样品指纹图谱的相似度均在0.8 以上，绿原酸和黄
芩苷的线性范围分别为：0.256～0.768 μg 和0.184～0.920 μg， 加样回收率分别为97.16%和97.23%。结论所建立的
HPLC 简便、可靠，可用于该制剂的质量控制。 |
| 关键词: 双黄花片 绿原酸 黄芩苷 高效液相色谱法 指纹图谱 |
| DOI： |
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| 基金项目:湖南省教育厅科研项目（09C751）；湖南省中医药管理局科研项目（2009030）。 |
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| Study of HPLC fingerprints and content determinationof chlorogenic acid and baicalin in Shuanghuanghua tablet |
| WANG Hua1, CAI Ping2, WAN Dan2 |
| (1. First Affiliated Hospital，TCM University of Hunan 2. TCM Academy of Hunan) |
| Abstract: |
| Objective To study the HPLC fingerprints and content of chlorogenic acid and
baicalin in Shuanghuanghua tablets. Methods The fingerprints and content were analyzed by
HPLC method. The Inertsil ODS-2 C18 column (250 mm×4.6 mm，5 μm) was used with acetonitrile
-0.2％ phosphoric acid solution as mobile phase. Under the condition of gradient elution, the flow
rate was 1.0 mL/min, the UV detection wavelength was 280 nm, and the column temperature was
25 ℃. Results Ten marker peaks were separated by HPLC. The similarity factor of fingerprints for
10 batches of samples was over 0.8. The quantitative linear range of chlorogenic acid and baicalin
were 0.256~0.768 μg and 0.184~0.920 μg respectively, and the average recoveries were 97.16%
and 97.23% respectively. Conclusion The established method in this study on fingerprints of
Shuanghuanghua tablets and content determination of chlorogenic acid and baicalin is rapid, simple
and reliable. It can be used specifically for the quality control of Shuanghuanghua tablets. |
| Key words: Shuanghuanghua tablet chlorogenic acid baicalin HPLC fingerprint |
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