| 引用本文: |
陈伶利； 李杰； 贺气志； 陈辉； 毛平道； 邓乐；.基于核酸适配体-荧光染料EvaGreenTM快速检测ATP的研究[J].湖南中医药大学学报,2011,31(5):6-9[点击复制] |
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| 基于核酸适配体-荧光染料EvaGreenTM快速检测ATP的研究 |
| 陈伶利；李杰；贺气志；陈辉；毛平道；邓乐； |
| (湖南师范大学生命科学学院; 湖南中医药大学基础医学院) |
| 摘要: |
| 目的利用荧光嵌入染料EvaGreenTM在单双链DNA溶液中不同的荧光性质构建基于核酸适配体的ATP检测体系,建立一种简单、高效的检测ATP的新方法。方法利用ATP核酸适配体双链DNA(dsDNA)和核酸绿色荧光染料Eva GreenTM嵌合作用,实现了对ATP的检测。考察了ATP的孵育温度、pH、浓度等因素对荧光强度的影响,并对该方法的特异性进行了验证。结果反应体系在12℃,pH 7.5条件下具有最佳实验效果,在最优条件下,最低能检测10-6mol/L的ATP,并具有较高的特异性。结论本研究为ATP的快速检测提供了一种易于操作、低成本的新方法。 更多还原 |
| 关键词: 核酸适配体 EvaGreenTM ATP 检测 |
| DOI： |
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| 基金项目:国家科技部863计划资助项目(2007AA062Z403) |
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| Study of rapid detecting ATP based on aptamer and DNA-specific dye EvaGreenT}'I |
| CHEN Ling-li,LI Jie,HE Qi-zhi,CHEN Hui,MAO Ping-dao1,DENG Le(College of Life Science,Hunan Normal University,Changsha,Hunan 410081,China) |
| ((College of Life Science, Hunan Normal University, Changsha, Hunan 410081, China)) |
| Abstract: |
| Objective To develop a simple, efficient fluorescence detection technology for ATP. Methods The DNA-intercalating dye Eva Greenr"' aptamer-ATP complex was used[()detect quickly ATP. The effects of temperature, concentration, pH and reaction time were studied. Re-cults The reacting system was that the best temperature and pH were 12 0C and pH7.5, The ATP at limitation 10一6 mol/L was detected with optimized conditions. Conclusion This research provides a kind of easy operation and low cost method for ATP rapid detection. |
| Key words: Aptamer Eva Green'" ATP detection |
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