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孙璐,谭静,阳仁达,言芳,廖宗力,魏星,罗明.隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α蛋白及其mRNA表达的影响[J].湖南中医药大学学报,2018,38(11):1273-1277[Click to copy
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| This paper
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| 隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α蛋白及其mRNA表达的影响 |
| 孙璐,谭静,阳仁达,言芳,廖宗力,魏星,罗明 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察隔药饼灸对慢性支气管炎大鼠肺组织中肿瘤坏死因子-α（TNF-α）蛋白及其mRNA含量的影响。方法 将48只SD大鼠随机分为空白组、模型组、隔药饼灸组、隔纸垫灸组，每组12只。采取改良烟熏法复制慢性支气管炎大鼠模型，观察用隔药饼灸治疗后大鼠的一般情况，并与空白组、模型组、隔纸垫灸组进行对照，大鼠肺组织中的TNF-α mRNA的表达用RT-PCR法检测，肺组织中TNF-α的蛋白表达用Western blot法检测。结果 与模型组相比，隔药饼组和隔纸垫组大鼠肺及气管的病理损伤均有好转；隔药饼灸组和隔纸垫灸组都能使慢支大鼠肺组织TNF-α mRNA及TNF-α蛋白含量表达降低（P<0.05），且隔药饼灸组效果更好（P<0.05）。结论 隔药饼灸能改善慢支大鼠一般症状及气道炎症，提示其机制可能是通过下调TNF-α蛋白及其mRNA的表达达到治疗慢性支气管炎的目的。 |
| 关键词: 慢性支气管炎 隔药饼灸 肿瘤坏死因子α 肿瘤坏死因子α信使RNA |
| DOI：10.3969/j.issn.1674-070X.2018.11.011 |
| 投稿时间：2018-08-09 |
| 基金项目:湖南省教育厅创新平台项目（16K068）；湖南省大学生创新实验项目（017-132） |
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| Effect of Herbal Cake-Separated Moxibustion on the Expression of TNF-α Protein and Its mRNA in Lung Tissue of Chronic Bronchitis Model Rats |
| SUN Lu,TAN Jing,YANG Renda,YAN Fang,LIAO Zongli,WEI Xing,LUO Ming |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of herbal cake-separated moxibustion on the content of tumor necrosis factor-α (TNF-α) protein and its mRNA in the lung tissue of rats with chronic bronchitis (CB). Methods Forty-eight Sprague-Dawley rats were randomly and equally divided into four groups, including blank group, model group, herbal cake-separated moxibustion group, and paper pad-separated moxibustion group. CB model rats were prepared by modified fumigation. The general status of rats after herbal cake-separated moxibustion was observed and compared with that in the other three groups. The expression of TNF-α mRNA in rat lung was measured by RT-PCR, and the protein expression of TNF-α in lung tissues was measured by Western blot. Results The pathological injury of lung and trachea of rats in herbal cake-separated moxibustion group and paper pad-separated moxibustion group was improved compared with that in the model group. Herbal cake-separated moxibustion and paper pad-separated moxibustion can reduce the expression of TNF-α mRNA and TNF-α protein in the lung tissues of CB rats (P<0.05), and the effect of herbal cake-separated moxibustion was better (P<0.05). Conclusion Herbal cake-separated moxibustion can improve the general symptoms and airway inflammation of CB rats, possibly by down-regulating the expression of TNF-α protein and its mRNA. |
| Key words: chronic bronchitis herbal cake-separated moxibustion tumor necrosis factor-α tumor necrosis factor-α mRNA |
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