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邓石峰,过灵香,凌希,易细芹,祁芳,张泓.电针下合穴阳陵泉等对急性胆囊炎模型豚鼠血清TNF-α及胆囊组织nAchR α7 mRNA的影响[J].湖南中医药大学学报,2018,38(11):1243-1247[Click to copy
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| 电针下合穴阳陵泉等对急性胆囊炎模型豚鼠血清TNF-α及胆囊组织nAchR α7 mRNA的影响 |
| 邓石峰,过灵香,凌希,易细芹,祁芳,张泓 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208;郑州市康复医院, 河南 郑州 450007;广西中医药大学针灸推拿学院, 广西 南宁 530200) |
| 摘要: |
| 目的 对比观察电针阳陵泉、上巨虚、下巨虚、足三里穴对急性胆囊炎（AC）模型豚鼠血清肿瘤坏死因子-α（TNF-α）及胆囊组织中烟碱型乙酰胆碱受体α7信使核糖核酸（nAchR α7 mRNA）表达的影响，探讨胆之下合穴阳陵泉治疗对应腑病是否存在相对特异性。方法 72只健康豚鼠随机分为空白、模型、阳陵泉、上巨虚、下巨虚、足三里组六组，12只/组，雌雄各半。除空白组外均于胆囊注射大肠杆菌建立豚鼠急性胆囊炎模型，成模后予电针治疗5 d，取胆囊组织行HE染色，镜下观察形态学变化，ELISA法检测血清TNF-α水平，RT-PCR检测胆囊nAchR α7 mRNA的表达。结果 与空白组比较，HE染色镜下观察可见其他组胆囊组织出现不同程度的病理损伤，且nAChRα7mRNA表达明显降低（P<0.01），血清中TNF-α明显升高（P<0.01）；与模型组比较，各电针治疗组血清TNF-α含量均明显降低（P<0.01）而胆囊nAChRα7mRNA表达均明显升高（P<0.01）；与阳陵泉组比较，足三里、上巨虚、下巨组血清TNF-α含量均明显升高（P<0.01）而胆囊nAChRα7mRNA表达均明显降低（P<0.01）。结论 针刺与消化系相关的内腑下合穴对AC模型豚鼠均可产生不同程度的治疗作用，其机制可能是通过调节TNF-α及nAchR α7 mRNA表达而抑制炎症因子、减轻炎症反应；对比可知，阳陵泉组的干预效应较其它治疗组突出，说明阳陵泉穴与对应胆腑之间存在相对特异性。 |
| 关键词: 急性胆囊炎 电针 下合穴 肿瘤坏死因子-α 乙酰胆碱受体α7信使核糖核酸 合治内府 |
| DOI：10.3969/j.issn.1674-070X.2018.11.005 |
| 投稿时间：2018-08-10 |
| 基金项目:国家自然科学基金项目（81173327/H2718） |
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| Effects of Electroacupuncture at Yanglingquan and Other Lower He-Sea Points on Serum TNF-α and Gallbladder nAChR α7 mRNA in Guinea Pig Model of Acute Cholecystitis |
| DENG Shifeng,GUO Lingxiang,LING Xi,YI Xiqin,QI Fang,ZHANG Hong |
| (School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Rehabilitation Hospital of Zhengzhou, Zhengzhou, Henan 450007, China;School of Acupuncture, Moxibustion & Tuina, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China) |
| Abstract: |
| Objective To compare the effects of electroacupuncture at Yanglingquan, Shangjuxu, Xiajuxu, and Zusanli points on serum tumor necrosis factor-α (TNF-α) and mRNA expression of α7 nicotinic acetylcholine receptor (nAChR α7) in the gallbladder in a guinea pig model of acute cholecystitis (AC), and to explore whether Yanglingquan, the lower He-sea point of the gallbladder, is relatively specific for treating the corresponding visceral disease. Methods Seventy-two healthy guinea pigs were randomly and equally divided into blank group, model group, Yanglingquan group, Shangjuxu group, Xiajuxu group, and Zusanli group, with equal numbers of male and female in each group. A model of AC was established by injection of Escherichia coli into the gallbladder of all guinea pigs except for the blank group. After model establishment, the four treatment groups received electroacupuncture for 5 days. The gallbladder was collected for HE staining and morphological analysis by microscopy. The serum level of TNF-α and expression of nAChR α7 mRNA in the gallbladder were measured by enzyme-linked immunosorbent assay and RT-PCR, respectively. Results Compared with the blank group, the other groups had varying degrees of pathological damages in the gallbladder according to HE staining and microscopic observation, as well as significantly lower expression of nAChR α7 mRNA and a significantly higher serum level of TNF-α (P<0.01). Compared with the model group, the four electroacupuncture groups had a significantly lower serum level of TNF-α and significantly higher expression of nAChR α7 mRNA in the gallbladder (P<0.01). Compared with the Yanglingquan group, the Shangjuxu group, the Xiajuxu group, and the Zusanli group had a significantly higher serum level of TNF-α and significantly lower expression of nAChR α7 mRNA in the gallbladder (P<0.01). Conclusion Electroacupuncture at the lower He-sea points of digestion-related organs has different degrees of therapeutic effects on the guinea pig model of AC, probably by regulating TNF-α and the expression of nAChR α7 mRNA and then inhibiting inflammatory factors and reducing inflammatory reaction. According to the comparative study, the Yanglingquan group has a better intervention effect than other treatment groups, suggesting that Yanglingquan point is specific for the gallbladder. |
| Key words: acute cholecystitis electroacupuncture lower He-sea point tumor necrosis factor-α α7 nicotinic acetylcholine receptor mRNA treating visceral diseases with He-sea point |
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