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谷捷,李鑫,余黄合,王宝新,黄惠勇,曾嵘,蔡雄.二甲苯致小鼠耳肿胀急性炎症模型的建立[J].湖南中医药大学学报,2016,36(5):32-35[Click to copy
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| 二甲苯致小鼠耳肿胀急性炎症模型的建立 |
| 谷捷,李鑫,余黄合,王宝新,黄惠勇,曾嵘,蔡雄 |
| (湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学药学院, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学中医方证研究转化医学湖南省高校重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过对二甲苯的用量和保留时间进行研究，优选二甲苯致小鼠耳肿胀急性炎症模型的最佳成模条件。方法 二甲苯涂抹昆明系小鼠右耳廓上下两面，诱导建立急性耳肿胀，对二甲苯剂量（20、30、40 μL）和保留时间（30、45、60、75、90 min）进行优选，并采用阳性药对优选建立的小鼠耳肿胀急性炎症模型进行验证。结果 二甲苯用量对鼠耳肿胀率影响不明显（P>0.05）；但随保留时间延长，耳肿胀度呈下降趋势，且90 min组与30 min组比较有显著性差异（P=0.045）。与模型组比较，经吲哚美辛治疗后，耳肿胀度显著降低（P=0.007），TNF-α含量亦明显下降（P<0.001）。结论 二甲苯用量20 μL，保留时间30 min，可成功建立最佳的小鼠耳肿胀急性炎症模型。 |
| 关键词: 二甲苯 耳肿胀急性炎症 致炎剂用量 保留时间 |
| DOI：10.3969/j.issn.1674-070X.2016.05.008 |
| 投稿时间：2015-07-03 |
| 基金项目:国家自然科学基金项目（81373540）；湖南省中医药科研计划项目（201308）；湖南省教育厅科学研究重点项目（12A107）；湖南中医药大学中医诊断国家重点学科开放基金重点项目（2014-8）；湖南省大学生创新性实验计划项目（2014202）。 |
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| Establishment of Acute Inflammation Mice Models with Ear Edema Induced by Dimethylbenzene |
| GU Jie,LI Xin,YU Huanghe,WANG Baoxin,HUANG HuiYong,ZENG Rong,CAI Xiong |
| (Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Hunan University Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Hunan University Chinese Medicine, Changsha, Hunan 410208, China;School of Pharmacy, Hunan University Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Hunan University Chinese Medicine, Changsha, Hunan 410208, China;Hunan Department of Education's Key Laboratory of Translational Chinese Medicine, Hunan University Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the optimal dosage and retention time of dimethylbenzene for the induction of a mouse ear edema. Methods Dimethylbenzene was applied evenly on the surface of both sides of the auricle of mice to induce acute inflammation. Different dosages (20, 30, 40 μL) and retention time (30, 45, 60, 75, 90 min) of dimethylbenzene were compared to optimize these main variables for the induction of ear edema in mice. For verifing the model parameters a positive drug was performed. Results Increase in the dosage of dimethylbenzene resulted in no significant increase in the ear edema (P>0.05); while the ear edema rate was decreased with the retention time prolonged, and marked reduction in the rate of ear edema was attained between 30 and 90 min. Compared with the vehicle-control mice, the ear edema rate (P=0.007) and serum values of TNF-α (P<0.001) decreased significantly in mice treated with indomethacin. Conclusion The optimal mouse model of ear edema is induced by applying 20 μL dimethylbenzene with 30 min retention. |
| Key words: dimethylbenzene acute inflammation of ear edema dosage retention time |
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