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陈心,杜清,尤昭玲,梁惠珍.补肾助卵汤上调雷公藤多苷诱导的卵巢功能低下大鼠的卵巢PLGF及Flt-1水平[J].湖南中医药大学学报,2021,41(11):1683-1688[点击复制] |
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| 补肾助卵汤上调雷公藤多苷诱导的卵巢功能低下大鼠的卵巢PLGF及Flt-1水平 |
| 陈心,杜清,尤昭玲,梁惠珍 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 研究补肾助卵汤对卵巢储备功能下降（DOR）模型大鼠卵巢组织中胎盘生长因子（PLGF）及其受体fms样酪氨酸激酶1（Flt-1）表达水平的影响。方法 选取9只动情周期正常的大鼠，随机分为3组，每组3只，空白组给予蒸馏水灌胃，模型组及补肾助卵汤组给予雷公藤多苷片悬浮液，连续14 d进行灌胃造模。观察大鼠动情周期，出现动情周期紊乱提示造模成功。空白组给予生理盐水；模型组给予雷公藤多苷片5 mg/（kg·d）及生理盐水；补肾助卵汤组给予雷公藤多苷片5 mg/（kg·d）及补肾助卵汤24 g/（kg·d）。观察大鼠性周期变化，免疫组化检测和Western blot检测大鼠卵巢组织PLGF和Flt-1的表达水平。结果 模型组大鼠动情周期出现延长、不规则甚至紊乱现象。补肾助卵汤组动情周期正常，与空白组相比，动情周期相对延长；与模型组相比，动情周期有所恢复。3组大鼠卵巢组织中细胞核和细胞质中均有PLGF和Flt-1表达，与空白组相比，模型组大鼠卵巢功能下降后PLGF和Flt-1蛋白表达水平下降（P<0.05）；与模型组相比，补肾助卵汤组上调大鼠卵巢组织中PLGF和Flt-1蛋白表达水平（P<0.05）。结论 补肾助卵汤可调节雷公藤多苷所致DOR大鼠动情周期，并对卵巢功能下降大鼠卵巢组织PLGF及Flt-1水平有一定的改善效果。 |
| 关键词: 卵巢功能低下 补肾助卵汤 胎盘生长因子 fms样酪氨酸激酶1 |
| DOI：10.3969/j.issn.1674-070X.2021.11.007 |
| 投稿时间：2021-08-24 |
| 基金项目:湖南省自然科学基金青年基金项目（2018JJ3398）。 |
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| Bushen Zhuluan Decoction Up-regulates the Levels of PLGF and Flt-1 in the Ovaries of Rats with Low Ovarian Function Induced by Tripterygium Wilfordii Polyglycosides |
| CHEN Xin,DU Qing,YOU Zhaoling,LIANG Huizhen |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To study the effect of Bushen Zhuluan Decoction on the expression of placenta growth factor (PLGF) and it's receptor fms-like tyrosine kinase l (Flt-1) in the ovarian tissue of diminished ovarian reserve (DOR) model rats. Methods 9 animals with normal estrus cycle were selected and randomly divided into 3 groups, with 3 animals in each group. The blank group was given distilled water gavage, and the model group and the Bushen Zhuluan Decoction group were given tripterygium wilfordii polyglycoside suspension for 14 consecutive days to establish model. The estrous cycle of rats was observed, the appearance of estrous cycle disorder indicated that the model was successful. The blank group was given normal saline; the model group was given tripterygium wilfordii polyglycoside tablets 5 mg/(kg·d) and normal saline; the Bushen Zhuluan Decoction group was given tripterygium wilfordii polyglycosides tablets 5 mg/(kg·d) and Bushen Zhuluan Decoction 24 g/(kg·d). The changes of rat sex cycle was observed. Immunohistochemistry and Western blot were used to detect the expression levels of PLGF and Flt-1 in rat ovarian tissue. Results The estrus cycle of rats in the model group was prolonged, irregular and even disordered. The estrus cycle of the Bushen Zhuluan Decoction group was normal, the estrus cycle was relatively prolonged than blank group; compared with the model group, the estrus cycle was restored. The nucleus and cytoplasm of the three groups of rat ovarian tissues both PLGF and Flt-1 were expressed. Compared with the blank group, the expression of PLGF and Flt-1 protein in the model group decreased significantly after the ovarian function decreased (P<0.05); compared with the model group, Bushen Zhuluan Decoction group significantly up-regulated the expression levels of PLGF and Flt-1 protein in the ovarian tissue of rats (P<0.05). Conclusion Bushen Zhuluan Decoction can regulate estrous cycle of DOR rats induced by tripterygium glycosides, and improve the levels of PLGF and Flt-1 in ovarian tissues of rats with reduced ovarian function. |
| Key words: decreased ovarian function Bushen Zhuluan Decoction placenta growth factor fms-like tyrosine kinase l |
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