| 引用本文: |
王孟迪,姚勤伟,薛涛,刘新民,梁娟娟,秦文杰,叶祖光,王琼.3种抗抑郁中药方作用比较研究[J].湖南中医药大学学报,2021,41(9):1327-1333[点击复制] |
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| 3种抗抑郁中药方作用比较研究 |
| 王孟迪,姚勤伟,薛涛,刘新民,梁娟娟,秦文杰,叶祖光,王琼 |
| (西南医科大学附属中医医院中葡中医药国际合作中心/药学院, 四川 泸州 646000;首都医科大学附属北京佑安医院重症肝病科, 北京 100069;西南医科大学附属中医医院中葡中医药国际合作中心/药学院, 四川 泸州 646000;中国医学科学院北京协和医学院药用植物研究所药理毒理研究中心, 北京 100193;北京振东光明药物研究院, 北京 100088;中国中医科学院中药研究所, 北京 100700;西南医科大学附属中医医院中葡中医药国际合作中心/药学院, 四川 泸州 646000;中国农业科学院农产品加工研究所, 北京 100193) |
| 摘要: |
| 目的 比较3个中药处方的抗抑郁作用，筛选出抗抑郁效果最好的处方。方法 ICR雄性小鼠（130只）随机分为对照组，模型组，阿米替林组，帕罗西汀组，处方一高、中、低浓度组，处方二高、中、低浓度组，处方三高、中、低浓度组，每组10只。通过采用小鼠悬尾实验、强迫游泳实验以及建立小鼠利血平拮抗模型，检测其眼睑下垂度、体温和自主活动能力，以此检测小鼠的抑郁样行为。结果 预防性给药7 d后，与对照组相比，阳性药及3个处方均能不同程度缩短小鼠悬尾不动时间（P<0.05，P<0.01）；阿米替林、处方二能显著缩短强迫游泳不动时间（P<0.05），增加游泳距离（P<0.01），但处方一和三无效。预防给药14 d后，与对照组相比，各阳性药与3个处方均能显著缩短小鼠悬尾不动时间（P<0.05，P<0.01）；同时，各阳性药与处方一和处方二能明显缩短小鼠强迫游泳不动时间（P<0.05，P<0.01），增加小鼠游泳距离（P<0.05），但处方三无效。在利血平拮抗实验中，与对照组相比，模型组运动时间明显减少（P<0.01），体温出现明显下降（P<0.01），小鼠眼睑下垂度明显增加（P<0.01）；与模型组相比，阿米替林、帕罗西汀以及3个处方均能不同程度逆转利血平所致小鼠运动不能（P<0.05），体温下降（P<0.05，P<0.01），眼睑下垂（P<0.05，P<0.01）的现象。结论 3个中药方均具有一定的抗抑郁作用，其中处方二的药理作用更广，抗抑郁作用效果更好，表现为起效时间短、药效强。 |
| 关键词: 抑郁症 中药方 悬尾 强迫游泳 利血平 |
| DOI：10.3969/j.issn.1674-070X.2021.09.003 |
| 投稿时间：2021-04-07 |
| 基金项目:四川省国际科技创新合作/港澳台科技创新合作项目(2019YFH0023)；泸州市高端人才引进项目（刘新民团队）；（泸州-医科大）应用基础研究项目（2018LZXNYD-ZK32）。 |
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| The Comparison of Three Traditional Chinese Medicine Formulas on Their Antidepressant Efficacy |
| WANG Mengdi,YAO Qinwei,XUE Tao,LIU Xinmin,LIANG Juanjuan,QIN Wenjie,YE Zuguang,WANG Qiong |
| (Affiliated TCM Hospital, Sino-Portugal TCM International Cooperation Center/School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China;Department of Critical Care Medicine of Liver Diseases, Beijing You'An Hospital, Capital Medical University, Beijing 100069, China;Affiliated TCM Hospital, Sino-Portugal TCM International Cooperation Center/School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Zhendong Pharmaceutical Research Institute, Beijing 100088, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Affiliated TCM Hospital, Sino-Portugal TCM International Cooperation Center/School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China;Institute of Food Science and Technology CAAS, Beijing 100193, China) |
| Abstract: |
| Objective To compare the effects of three traditional Chinese medicine (TCM) formulas on their antidepressent efficacy and to screen out the prescription with the best antidepressant effect. Methods 130 ICR male mice were randomly divided into 13 groups, including the control group, the model group, the amitriptyline group, the paroxetine group, the first formula groups (high, medium and low dosages), the second formula groups (high, medium and low dosages), the third formula groups (high, medium and low dosages). 10 mice in each group. Tail suspension test, forced swimming test and reserpine induced depression mice model were used. The degree of eyelid ptosis, body temperature and autonomous activity were measured to test the depressive-like behavior in mice. Results Prophylaxis and treatment of TCM formulas for 7 days, compared with the control group, the positive medicine and the three formulas significantly shorten the immobility time in the tail suspension test in mice (P<0.05, P<0.01); amitriptyline and the second formula significantly shorten the immobility time of forced swimming (P<0.05) and increased swimming distanse (P<0.01). The first formula and third formula had no effect in the forced swimming test in mice. Prophylaxis and treatment of TCM formulas for 14 days, compared with the control group, the positive medicines and the three formulas significantly shorten the immobility time in the tail suspension test (P<0.05, P<0.01). At the same time, positive medicines and the first and the second formulas significantly shorten the immobility time of forced swimming (P<0.05, P<0.01) and increased swimming distanse (P<0.05), but the third formula had no effect in the forced swimming test in mice. In the reserpine antagonistic experiment, compared with the control group, the movement time of the model group was significantly reduced (P<0.01), the body temperature was significantly decreased (P<0.01), and the ptosis degree of the mouse eyelid was significantly increased (P<0.01). Compared with the model group, amitriptyline, paroxetine and three prescriptions could reverse the locomotor failure (P<0.05), body temperature decreased (P<0.05, P<0.01), eyelid ptosis (P<0.05, P<0.01) induced by reserpine to varying degrees. Conclusion Three TCM formulas have effects on antidepression, but the second formula is the best one which has fast and strong effects on antidepression compared to the other two formulas. |
| Key words: depression traditional Chinese medicine formula tail suspension forced swimming test reserpine |
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