| 引用本文: |
彭卓隽,林亚平,谭静,赵欢,陈欲攀,石峻林.艾灸对胃荷瘤大鼠生存状态及骨骼肌代谢的影响[J].湖南中医药大学学报,2019,39(12):1488-1492[点击复制] |
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| 艾灸对胃荷瘤大鼠生存状态及骨骼肌代谢的影响 |
| 彭卓隽,林亚平,谭静,赵欢,陈欲攀,石峻林 |
| (湖南中医药大学研究生院, 湖南 长沙 410208;湖南中医药大学针灸推拿学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过观察艾灸对荷瘤大鼠生存状态及对骨骼肌代谢的影响，探讨艾灸对荷瘤大鼠胃肿瘤的影响。方法 将40只SD大鼠随机选取其中20只分为正常组、假手术组各10只，其余20只采用Walker-256型瘤组织胃部黏贴制备胃癌模型，将模型制备成功的16只大鼠再随机分为模型组和艾灸组各8只。各组均每日捆绑20 min，艾灸组每日穴位施灸20 min，共干预14 d。每日观察大鼠生存状态，记录生存状态积分和体质量；称取取材前体质量以及腓肠肌质量；使用游标卡尺测量肿瘤的最大径和最小径，电子天平称量肿瘤体质量，计算肿瘤体积抑制率；TUNEL法观察腓肠肌细胞凋亡情况；HE染色后观察腓肠肌细胞并测量横径。结果 与模型组相比起：艾灸组生存状态改善，生存症状积分降低（P<0.05）；艾灸8 d后体质量均明显高于模型组体质量（P<0.05）；胃部瘤体质量和瘤体体积均明显减小（P<0.01）；腓肠肌细胞凋亡指数均降低（P<0.05），腓肠肌质量明显提高（P<0.01），腓肠肌横径长度增加（P<0.05）。结论 艾灸能在一定程度上抑制胃癌大鼠肿瘤的生长，改善生存状态，减少骨骼肌细胞过度消耗。 |
| 关键词: 胃荷瘤 艾灸 生存状态 腓肠肌质量 骨骼肌代谢 大鼠 |
| DOI：10.3969/j.issn.1674-070X.2019.12.015 |
| 投稿时间：2019-09-04 |
| 基金项目:国家自然科学基金项目（81574077）；湖南省教育厅优秀青年项目（18B257）。 |
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| Effects of Moxibustion on Survival Status and Skeletal Muscle Metabolism in Rats with Gastric Tumor |
| PENG Zhuojun,LIN Yaping,TAN Jing,ZHAO Huan,CHEN Yupan,SHI Junlin |
| (Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Acupuncture and Massage, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective By observing the effects of moxibustion on the survival status of tumor-bearing rats and the metabolism of skeletal muscle, and to explore the effects of moxibustion on gastric tumors in tumor-bearing rats. Methods A total of 20 rats were randomly selected from 40 SD rats and divided into a normal group and an sham operation group, with 10 rats in each group. The remaining 20 rats were prepared gastric cancer models by Walker-256 tumor tissue gastric sticking. The 16 rats successfully modeling were randomly divided into a model group and a moxibustion group, with 8 rats in each group. Each group was bundled for 20 min every day, and the moxibustion group applied moxibustion for 20 minutes per day for a total of 14 days. The survival status of the rats was observed daily, and the survival status scores and body weights were recorded. The body weight and the weight of the gastrocnemius before taking materials were weighed; The maximum and minimum diameter of the tumor was measured using a vernier caliper. The tumor mass was weighed by electronic balance, and the tumor volume inhibition rate was calculated; The apoptosis of myocytes was observed by TUNEL method; The gastrocnemius muscle cells were observed after HE staining and Horizontal diameter was measured. Results Compared with the model group, the survival status of the moxibustion group improved and the score decreased (P<0.05). The body mass after 8 days of moxibustion was significantly higher than that of the model group (P<0.05); tumor weight and tumor volume were significantly decreased (P<0.01); gastrocnemius muscle fiber apoptosis index decreased (P<0.05), gastrocnemius weight increased significantly (P<0.01), gastrocnemius transverse diameter length increased (P<0.05). Conclusion Moxibustion can inhibit the growth of tumor in gastric cancer rats to a certain extent, improve the living condition and reduce the excessive consumption of skeletal muscle cells. |
| Key words: gastric-bearing tumor rats moxibustion survival state gastrocnemius muscle mass skeletal muscle metabolism rats |
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