| 引用本文: |
陈丽,邢琴,周忠志,刘沐琳,陈港军,李利玲,李旺.复方ANBP对小鼠创面组织学变化的实验研究[J].湖南中医药大学学报,2019,39(6):699-703[点击复制] |
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| 复方ANBP对小鼠创面组织学变化的实验研究 |
| 陈丽,邢琴,周忠志,刘沐琳,陈港军,李利玲,李旺 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察中药复方ANBP[由仙鹤草（Agrimonia Eupatoria，A）、藕节（Nelumbo Nucifera Gaertn，N）、乳香（Boswellia Carteri，B）和蒲黄（Pollen Typhae Angustifoliae，P）组成]对全层皮肤缺损小鼠创面愈合的影响，初步探讨复方ANBP对小鼠皮肤创面愈合的治疗机制。方法 将48只C57BL/6小鼠按照随机数字表法分为空白对照组、ANBP组，每组24只。采用全层打孔器制备小鼠背部全层皮肤缺损模型，每组再随机分为6 h、3 d、7 d、14 d 4个时间点。造模后空白对照组不做任何处理，ANBP组创面被覆复方ANBP粉末。通过肉眼和HE染色观察创面愈合情况并计算创面愈合率，对数据进行统计学分析。结果 （1）造模后3 d、7 d和14 d，与空白对照组相比，ANBP组创伤愈合率明显增加，差异具有统计学意义（P<0.05）；（2）HE染色结果显示ANBP组表皮舌长度增加、均匀平整，肉芽组织丰富。结论 复方ANBP可以明显改善小鼠创面情况，加快小鼠背部皮肤创面愈合速度，提高愈合质量。 |
| 关键词: 中药复方 皮肤缺损 创面愈合 组织形态 |
| DOI：10.3969/j.issn.1674-070X.2019.06.005 |
| 投稿时间：2018-11-12 |
| 基金项目:国家自然科学基金（81501669）；湖南省重点研发计划-国际与区域科技创新合作专题项目（2016WK2011）；湖南省普通高等学校教学改革研究项目（2016-336）；2017年国家级大学生创新创业训练计划项目（201710541003）；2017年度湖南省大学生研究性学习和创新性实验计划项目（312）；2017年湖南中医药大学生研究性学习和创新性实验计划课题（2017-14）。 |
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| Experimental Study on Histological Changes of Wound in Mice by Compound ANBP |
| CHEN Li,XING Qin,ZHOU Zhongzhi,LIU Mulin,CHEN Gangjun,LI Liling,LI Wang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effects of compound ANBP[(Agrimonia Eupatoria, A), (Nelumbo Nucifera Gaertn, N), (Boswellia Carteri, B) and (Pollen Typhae Angustifoliae, P)] on wound healing in mice with full-thickness skin defect, and preliminarily explore its related mechanism. Methods A total of 48 C57BL/6 mice were randomly divided into a blank control group and an ANBP group, with 24 mice in each group. And each group was also randomly divided into 4 time points of 6 h, 3 d, 7 d and 14 d to prepare the full-thickness skin defect model of mouse back by full-layer puncher. No treatment was given to the blank control group after modeling, and the wound was covered with compound ANBP powder in the ANBP group. The wound healing was observed by naked eye and HE staining and the wound healing rate was calculated. Also, the data were statistically analyzed. Results (1) Compared with the blank control group, the wound healing rate in the ANBP group was significantly increased after 3 d, 7 d and 14 d of the modeling, and the difference was statistically significant (P<0.05); (2) HE staining showed that the length of epidermal tongue was increased, even and flat, with abundant granulation tissue. Conclusion Compound ANBP can significantly improve the wound condition in mice, accelerate the healing speed of the skin wound on the mouse back, and improve the healing quality. |
| Key words: Chinese compound formula full-thickness skin defect wound healing histomorphology |
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