| 引用本文: |
罗向艳,罗萍,颜家朝,李传课,李波,陈向东.视网膜色素上皮细胞光损伤模型的建立与评估[J].湖南中医药大学学报,2017,37(6):599-601[点击复制] |
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| 视网膜色素上皮细胞光损伤模型的建立与评估 |
| 罗向艳,罗萍,颜家朝,李传课,李波,陈向东 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院眼科, 湖南 长沙 410007) |
| 摘要: |
| 目的 通过不同光照时间和光照强度，探索建立最佳视网膜色素上皮细胞光损伤模型，为视网膜光损伤细胞造模方法提供理论依据。方法 选取原代大鼠视网膜色素上皮细胞和大鼠视网膜色素上皮细胞系，LED白色冷光灯模拟自然光源，选取（2 500、5 000、7 500、10 000 Lux）作为光照强度，以不同光照时间（6、12 h） 对细胞进行照射，建立光损伤模型，镜下观察细胞形态，采用MTT法检测细胞活力。结果 光照处理后，RPE细胞活力受到抑制，随着光照强度及时间增强，细胞活力降低明显，原代细胞6 h 7 500、10 000 Lux和12 h 5 000、7 500、10 000 Lux，细胞系6 h 10 000 Lux和12 h 7 500、10 000 Lux，视网膜色素上皮细胞活力均低于空白组（P<0.01），差异有统计学意义。结论 RPE细胞活力受光照时间及强度抑制，原代细胞较细胞系对光损伤更敏感。 |
| 关键词: 视网膜色素上皮细胞 光损伤 光照时长 光照强度 原代细胞 细胞系 |
| DOI：10.3969/j.issn.1674-070X.2017.06.005 |
| 投稿时间：2016-12-06 |
| 基金项目:国家自然科学基金青年基金项目（81303007/H2713）；国家中医药管理局全国名老中医药专家李传课传承工作室（国中医药人教发2013（47）号）。 |
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| Establishment and Evaluation of Light-Induced Retinal Pigment Epithelial Cells Damage Models |
| LUO Xiangyan,LUO Ping,YAN Jiachao,LI Chuanke,LI Bo,CHEN Xiangdong |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To establish the best light-induced retinal pigment epithelial cells damage models through different light application time and light intensity, and provide the theoretical basis for building this model. Methods The primary retinal pigment epithelial cells and cell lines in rats were selected, LED cold light lamp simulate natural white light source, select 2 500, 5 000, 7 500, 10 000 lux as light intensity, with different illumination time (6 h, 12 h) irradiation on cells, light damage model was established. The cell morphology was observed by microscope, cell vitality was determined by MTT method. Results After light treatment, RPE cell vitality was restrained. The cell vitality reduced obviously with increasing intensity and time of light. The cell vitality of primary cell at 6 h 7 500, 10 000 lux, 12 h 5 000, 7 500, 10 000 lux, cell line at 6 h 10 000 lux and 12 h 7 500, 10 000 lux, was lower than the blank group, there were significant differences (P<0.01). Conclusion Retinal pigment epithelial cell vitality is inhibited by the time and intensity of light. The primary cell is more sensitive than cell lines on light-induced damage. |
| Key words: retinal pigment epithelial cells light-induced damage illumination time light intensity primary cell cell line |
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