| 引用本文: |
尹秀婷,张泓,张健,许明,刘寄生,林居财.不同时辰艾灸对急性胃黏膜损伤模型大鼠EGF及TGF-α的影响[J].湖南中医药大学学报,2017,37(3):294-297[点击复制] |
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| 不同时辰艾灸对急性胃黏膜损伤模型大鼠EGF及TGF-α的影响 |
| 尹秀婷,张泓,张健,许明,刘寄生,林居财 |
| (湖南中医药大学研究生院, 湖南 长沙 410208;湖南中医药大学康复教研室, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察不同时辰艾灸对急性胃黏膜损伤模型大鼠表皮生长因子（epidermal growth factor，EGF）及转化生长因子-α（transforming growth factor-α，TGF-α）的影响，探讨不同时辰艾灸对大鼠急性胃黏膜损伤修复是否存在差异，期以阐述施灸时机对艾灸疗效的影响。方法 40只SD大鼠随机分为空白组（A）、模型组（B）、辰时艾灸组（C）、未时艾灸组（D）、戌时艾灸组（E），每组8只，除A组以外，采用无水乙醇灌胃法将其余大鼠制备成急性胃黏膜损伤模型，其中A组、B组不予施灸；余三组选用两组穴位：（1）“足三里”（双）“中脘”；（2）“脾俞”（双）“胃俞”（双）施温和灸20 min。各组予以相应处理7 d后，取胃组织肉眼下观察胃黏膜损伤指数（UI），Elisa法检测胃黏膜组织中EGF、TGF-α的含量。结果 （1）相比于A组，其余各组UI值明显提高（P<0.01）；相比于B组，各艾灸组UI值明显降低（P < 0.05或P < 0.01）；艾灸组之间比较，C组、E组UI值低于D组（P<0.05）。（2）与B组相比，各艾灸组胃黏膜EGF、TGF-α含量显著增加（P < 0.05或P < 0.01）；艾灸组之间比较，C组胃黏膜EGF、TGF-α的含量高于D组、E组（P<0.05）。结论 （1）不同时辰艾灸“足三里”“中脘”“脾俞”“胃俞”对大鼠急性胃黏膜损伤具有一定的修复作用，可能是通过提高胃黏膜中EGF、TGF-α的表达发挥修复作用的；（2）以辰时艾灸组疗效最优，提示辰时艾灸存在相对特异性，部分证实施灸时机对艾灸效应的影响。 |
| 关键词: 时辰 急性胃黏膜损伤 艾灸 表皮生长因子 转化生长因子-α |
| DOI：10.3969/j.issn.1674-070X.2017.03.017 |
| 投稿时间：2016-11-13 |
| 基金项目:国家自然科学基金（85010001031）；湖南省研究生科研创新课题项目（0141-090100200311）。 |
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| Effects of Different Time Moxibustion on EGF and TGF-α in Acute Gastric Mucosal Lesion Model Rats |
| YIN Xiuting,ZHANG Hong,ZHANG Jian,XU Ming,LIU Jisheng,LIN Jucai |
| (Graduate School of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Department of Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of different time moxibustion on EGF and TGF-α contents in acute gastric mucosal lesion model rats, and to investigate whether differences exsit in different time moxibustion on acute stomach membrane damage repair. To elabrate the effect of moxibustion at different time. Methods 40 SD rats were randonly divided into the control group (group A), model group (group B), moxibution at 7 a.m to 9 a.m group(group C), moxibution at 1 p.m to 3 p.m group (group D), moxibution at 7 p.m to 9 p.m group (group E), eight rats in each group. The gastric mucosal injury rats models besides group A were built by intragastric administration of absolute alcohol. Group A and B were not given moxibustion. The other three groups were treated with mild moxibustion for 20 min at two sets of acupoints, (1) "Zusanli"(two), "Zhongwan", (2) "Pishu"(two), "Weishu"(two). After treatment for 7 d, the UI (gastric mucosal injury index) of gastric tissue was observed under naked eyes. The EGF and TGF-α in gastric tissue were determined by Elisa. Results (1) Compared with group A, the UI index in other groups increased significantly (P<0.01). Compared with group B, UI index in other groups decreased markedly (P < 0.01 or P < 0.05). The UI of group C and group E was lower than that of group D (P<0.05). (2) Compared with group B, EGF and TGF-α in the groups increased significantly (P < 0.01 or P < 0.05), while the EGF and TGF-α in group C were higher than that in group D and group E (P<0.05). Conclusion (1) Different time moxibustion at "Zusanli" "Zhongwan" "Pishu" and "Weishu" have repairing effect on acute gastric mucosal injury rats, its repairing effect was probably played by up-regulating EGF and TGF-α protein expression. (2) The moxibution at the day of from 7 a.m to 9 a.m shows strongest effect on acute acute gastric mucosal lesion, which demonstrate that there is a relative specificity of different time moxibustion on the effect of moxibustion, partially confirm time on moxibustion effect. |
| Key words: time acute gastric mucosal lesions moxibustion EGF TGF-α |
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