| 引用本文: |
杨忠徐, 冯晓婷, 李庆龙, 周宗涛, 盛欣瑶, 林丽萍, 黄璐琦, 周融融, 张水寒.基于“痰浊-血瘀”证候视角的单细胞测序技术在中医药治疗COPD研究中的应用[J].湖南中医药大学学报,2026,46(5):1080-1088[点击复制] |
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| 基于“痰浊-血瘀”证候视角的单细胞测序技术在中医药治疗COPD研究中的应用 |
| 杨忠徐,冯晓婷,李庆龙,周宗涛,盛欣瑶,林丽萍,黄璐琦,周融融,张水寒 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院附属医院, 湖南 长沙 410006;南华大学, 湖南 衡阳 421001;中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室, 北京 100700;湖南省中医药研究院中药资源研究所, 湖南 长沙 410017) |
| 摘要: |
| 慢性阻塞性肺疾病（COPD）归属于中医学“肺胀”“喘证”范畴，“痰浊”与“血瘀”贯穿疾病发生发展全过程。中医药凭借多成分、多靶点协同调控优势，在改善COPD患者生活质量、减少急性发作频次等方面具有独特优势，但其作用机制仍缺乏系统性的微观阐释。单细胞RNA测序（scRNA-seq）技术的发展，为深入解析COPD肺组织细胞异质性及病理微环境动态变化提供了新视角。本文在梳理中医“痰浊-血瘀”理论的基础上，系统总结COPD中关键细胞亚群在黏液高分泌、慢性炎症和血管重塑中的作用，重点归纳scRNA-seq在细胞通讯网络及信号通路研究中的应用进展，并综述中医药通过调控关键细胞亚群及信号通路重塑肺部微环境的分子机制。旨在为中医传统病机理论提供现代细胞生物学佐证，为中医药防治COPD的精准干预及机制研究提供新思路。 |
| 关键词: 慢性阻塞性肺疾病|中医药|scRNA-seq技术|细胞异质性|辨证论治 |
| DOI：10.3969/j.issn.1674-070X.2026.05.030 |
| 投稿时间：2026-01-27 |
| 基金项目:国家自然科学基金项目（82405386）；名贵中药资源可持续利用能力建设项目（2060302-2304-02）。 |
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| Application of single-cell sequencing technology in the study of TCM treatment of COPD from the perspective of "phlegm turbidity-blood stasis" pattern |
| YANG Zhongxu, FENG Xiaoting, LI Qinglong, ZHOU Zongtao, SHENG Xinyao, LIN Liping, HUANG Luqi, ZHOU Rongrong, ZHANG Shuihan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China;University of South China, Hengyang, Hunan 421001, China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha, Hunan 410017, China) |
| Abstract: |
| Chronic obstructive pulmonary disease (COPD) falls under the categories of "lung distension" and "dyspnoea pattern" in Chinese medicine, with "phlegm turbidity" and "blood stasis" running through the entire course of disease onset and progression. Owing to its multi component and multi target synergistic regulatory advantages, TCM has unique strengths in improving the quality of life and reducing the frequency of acute exacerbations in COPD patients. However, its mechanism of action still lack systematic microscopic elucidation. The development of single cell RNA sequencing (scRNA-seq) technology provides a new perspective for in depth analysis of lung tissue cellular heterogeneity and dynamic changes in the pathological microenvironment in COPD. Based on the TCM theory of "phlegm turbidity-blood stasis", this paper systematically summarizes the roles of key cell subpopulations in mucus hypersecretion, chronic inflammation, and vascular remodeling in COPD. It highlights recent advances in scRNA-seq applications for studying cell-cell communication networks and signaling pathways. Furthermore, it reviews the molecular mechanisms by which TCM interventions regulate key cell subpopulations and signaling pathways to remodel the pulmonary microenvironment. The aim is to provide modern cytobiological evidence for the traditional TCM pathogenesis theories and to offer new insights for precise intervention and mechanistic research of TCM in the prevention and treatment of COPD. |
| Key words: chronic obstructive pulmonary disease|Chinese medicine|scRNA-seq technology|cellular heterogeneity|pattern differentiation and treatment |
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