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白雪敏, 岳炳南, 张静, 李松丽, 杨艺, 马玉梅, 刘清国.电针太冲、内关对自发性高血压大鼠心肌组织ELABELA、炎症因子及心肌纤维化的影响[J].湖南中医药大学学报,2025,45(8):1461-1468[点击复制] |
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| 电针太冲、内关对自发性高血压大鼠心肌组织ELABELA、炎症因子及心肌纤维化的影响 |
| 白雪敏,岳炳南,张静,李松丽,杨艺,马玉梅,刘清国 |
| (北京中医药大学针灸推拿学院, 北京 100029) |
| 摘要: |
| 目的 探讨电针太冲、内关对自发性高血压大鼠（SHR）心肌组织ELABELA表达、炎症反应及心肌纤维化的影响，阐明其改善高血压心脏损害的潜在机制。方法 12只SHR随机均分为模型组和针刺组，另选6只Wistar京都（WKY）大鼠作为空白组。针刺组电针干预双侧太冲、内关，电流强度设定为2 mA，频率为2 Hz/15 Hz，留针20 min。空白组和模型组仅模拟与针刺组同等时长的捉拿、固定操作。所有组别均每天干预1次，共干预15 d。分别于干预的第0、3、6、9、12、15天固定时间测量大鼠尾动脉收缩压。于干预第15天取材，采用HE和Masson染色观察大鼠心肌组织的病理变化；采用qRT-PCR检测大鼠心肌组织中ELABELA、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6) mRNA表达；采用ELISA检测大鼠心肌组织中ELABELA、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的蛋白含量。结果 与空白组相比，模型组大鼠尾动脉收缩压显著升高（P<0.01）,HE染色显示心肌组织结构紊乱，Masson染色显示心肌组织胶原蓝染面积增加、胶原沉积增多，TNF-α、IL-6 m RNA表达显著升高（P<0.01）,MMP-2、MMP-9蛋白含量显著升高（P<0.01）,ELABELA mRNA表达及蛋白含量显著降低（P<0.01）。与模型组相比，针刺组大鼠尾动脉收缩压于干预第6、9、12、15天均显著降低（P<0.01）,HE染色显示心肌组织结构紊乱程度下降，Masson染色显示心肌组织胶原蓝染面积减小、胶原沉积减少、纤维化程度减轻，TNF-α、IL-6 mRNA表达显著降低（P<0.01）,MMP-2、MMP-9蛋白含量降低（P<0.05,P<0.01）,ELABELA mRNA表达及蛋白含量均显著升高（P<0.01）。Pearson相关性分析结果显示，ELABELA与炎症因子TNF-α、IL-6及纤维化指标MMP-2、MMP-9之间存在显著负相关（P<0.01)。结论 电针双侧太冲、内关可以有效降低SHR的收缩压，改善SHR心肌的炎症反应与纤维化程度，其机制可能与上调心肌组织ELABELA表达，进而抑制炎症及纤维化相关因子有关。 |
| 关键词: 高血压 针刺 ELABELA 炎症反应 心肌纤维化 太冲 内关 |
| DOI：10.3969/j.issn.1674-070X.2025.08.009 |
| 投稿时间：2025-03-26 |
| 基金项目:国家自然科学基金项目(82074553);北京市自然科学基金项目(7242231) |
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| Effects of electroacupuncture at "Taichong （LR3）" and "Neiguan （PC6）" acupoints on ELABELA, inflammatory factors, and myocardial fibrosis in myocardial tissue of spontaneously hypertensive rats |
| BAI Xuemin, YUE Bingnan, ZHANG Jing, LI Songli, YANG Yi, Ma Yumei, LIU Qingguo |
| (School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China) |
| Abstract: |
| Objective To investigate the effects of electroacupuncture（EA） at "Taichong（LR3）" and "Neiguan（PC6）" acupoints on ELABELA expressions, inflammatory responses, and myocardial fibrosis in myocardial tissue of spontaneously hypertensive rats（SHR）, and to elucidate its potential mechanism for alleviating cardiac damage caused by hypertension. Methods Twelve SHR were randomly divided into model group（n=6） and EA group（n=6）. Six additional Wistar Kyoto（WKY） rats served as the blank group.The EA group received EA at bilateral "Taichong（LR3）" and "Neiguan（PC6）", with current intensity set at 2 mA, frequency of 2Hz/15 Hz, and needle retention for 20 min. Both blank and model groups only underwent sham manipulation, including handling and restraint for the same duration as EA group. All groups were intervened once a day for 15 consecutive days. The tail systolic blood pressure（SBP） of rats was measured at fixed times on days 0, 3, 6, 9, 12, and 15 of intervention. Samples were taken on the15th day of intervention. Pathological changes in rat myocardial tissue were observed by HE and Masson staining. The mRNA expressions of ELABELA, tumor necrosis factor-α（TNF-α）, and interleukin-6（IL-6） in rat myocardial tissue were measured by qRTPCR. The protein content of ELABELA, matrix metalloproteinase-2（MMP-2）, and matrix metalloproteinase-9（MMP-9） in rat myocardial tissue was determined by ELISA. Results Compared with the blank group, rats in the model group demonstrated significantly increased tail SBP（P＜0.01）, disordered myocardial tissue structure on HE staining, increased collagen blue staining area and collagen deposition in myocardial tissue on Masson staining, significantly elevated mRNA expressions of TNF-α and IL-6（P＜0.01）,significantly increased protein content of MMP-2 and MMP-9（P＜0.01）, and significantly decreased mRNA expression and protein content of ELABELA（P＜0.01）. Compared with the model group, rats in the EA group exhibited significantly decreased tail SBP on the 6th, 9th, 12th, and 15th days of intervention（P＜0.01）, decreased degree of disordered myocardial tissue structure on HE staining,reduced collagen blue staining area, decreased collagen deposition, and alleviated fibrosis degree on Masson staining, significantly decreased mRNA expressions of TNF-α and IL-6（P＜0.01）, decreased protein content of MMP-2 and MMP-9（P＜0.05, P＜0.01）, and significantly increased mRNA expression and protein content of ELABELA（P＜0.01）. The Pearson correlation analysis results showed significant negative correlations between ELABELA and inflammatory factors TNF-α, IL-6, as well as fibrosis indicators MMP-2and MMP-9（P＜0.01）. Conclusion EA at bilateral "Taichong（LR3）" and "Neiguan（PC6）" acupoints effectively reduces SBP, ameliorates myocardial inflammation and fibrosis in SHR. Its mechanism may be associated with the restraint of relevant inflammatory and fibrotic factors by increasing ELABELA expression in myocardial tissue. |
| Key words: hypertension acupuncture ELABELA inflammatory response myocardial fibrosis "Taichong（LR3）" "Neiguan（PC6）" |
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