| 引用本文: |
王琦, 席建元, 张玉凤, 谢汶芳, 祁林, 羊羡, 陈邦第, 李小鹏.基于STAT3/Survivin通路探讨银屑平丸含药血清对NETs诱导HaCat细胞增殖迁移作用的影响[J].湖南中医药大学学报,2025,45(8):1427-1433[点击复制] |
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| 基于STAT3/Survivin通路探讨银屑平丸含药血清对NETs诱导HaCat细胞增殖迁移作用的影响 |
| 王琦,席建元,张玉凤,谢汶芳,祁林,羊羡,陈邦第,李小鹏 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探索银屑平丸抑制信号转导及转录激活因子3(STAT3)/Survivin信号通路在中性粒细胞胞外诱捕网（NETs）诱导HaCat细胞增殖迁移作用中的相关机制。方法 从银屑病（PsO）患者及健康体检者（HC）外周静脉血中分离出对应NETs，参照中药血清药理学方法制备空白血清、银屑平丸及阿维A含药血清。将HaCat细胞分为对照组、HC-NETs组、PsO-NETs组、空白血清组、银屑平丸含药血清组、阿维A含药血清组、银屑平丸含药血清+Colivelin TFA组。通过CCK-8法、细胞划痕实验检测各组HaCat细胞增殖、迁移能力；Western blot法检测STAT3、p-STAT3、Survivin蛋白表达水平。结果 与对照组相比，PsO-NETs组、空白血清组细胞增殖能力、细胞迁移率明显升高（P<0.01）,STAT3、p-STAT3、Survivin蛋白表达水平明显升高（P<0.01）；与PsO-NETs组相比，银屑平丸含药血清组细胞增殖能力、细胞迁移率明显降低（P<0.01）,STAT3、p-STAT3、Survivin蛋白表达水平均降低（P<0.05,P<0.01）；与银屑平丸含药血清组相比，银屑平丸含药血清组+Colivelin TFA组细胞增殖能力、细胞迁移率明显升高（P<0.01）,STAT3、p-STAT3、Survivin蛋白表达水平均升高（P<0.05,P<0.01）。结论 银屑平丸含药血清可降低NETs诱导HaCat细胞的增殖迁移能力，其作用机制可能与抑制STAT3/Survivin信号通路有关。 |
| 关键词: 银屑病 银屑平丸 中性粒细胞胞外诱捕网 STAT3/Survivin信号通路 人角质形成细胞 |
| DOI：10.3969/j.issn.1674-070X.2025.08.005 |
| 投稿时间：2025-03-13 |
| 基金项目:湖南省科技创新计划项目(2021SK51411);湖南省自然科学基金部门联合基金项目(2023JJ60490);长沙市自然科学基金项目(kq2202457) |
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| Effects of Yinxieping Pill-containing serum on NETs-induced proliferation and migration of HaCaT cells via the STAT3/Survivin pathway |
| WANG Qi, XI Jianyuan, ZHANG Yufeng, XIE Wenfang, QI Lin, YANG Xian, CHEN Bangdi, LI Xiaopeng |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To explore the mechanism by which Yinxieping Pill（YXPP）-containing serum inhibits the signal transducer and activator of transcription3（STAT3）/Survivin signaling pathway in neutrophil extracellular traps（NETs）-induced proliferation and migration of HaCaT cells. Methods NETs were isolated from the peripheral venous blood of psoriasis（PsO）patients and healthy controls（HC）. Blank serum, YXPP-containing serum, and acitretin-containing serum were prepared following the method of serum pharmacology in Chinese medicine. HaCaT cells were assigned into the control group, HC-NETs group, PsONETs group, blank serum group, YXPP-containing serum group, acitretin-containing serum group, and YXPP-containing serum +colivelin TFA group. The proliferation and migration abilities of HaCaT cells in each group were checked by CCK-8 assay and scratch wound healing assay. The protein expression levels of STAT3, p-STAT3, and Survivin were measured by Western blot. Results Compared with the control group, the prolliferation ability, migration rate, and protein expression levels of STAT3, p-STAT3,and Survivin were significantly increased in the PsO-NETs group and blank serum group（P＜0.01）. Compared with the PsO-NETs group, the YXPP-containing serum group showed significantly reduced proliferation ability, migration rate, and protein expression levels of STAT3, p-STAT3, and Survivin（P＜0.05, P＜0.01）. Compared with the YXPP containing serum group, the YXPP-containing serum + Colivelin TFA group showed significantly higher cell proliferation ability and migration rate（P＜0.01）, and protein expression levels of STAT3, p-STAT3, and Survivin（P＜0.05, P＜0.01）. Conclusion YXPP-containing serum can inhibit NETs-induced proliferation and migration of HaCaT cells, and its mechanism of action may be related to the suppression of the STAT3/Survivin signaling pathway. |
| Key words: psoriasis Yinxieping Pill neutrophil extracellular traps STAT3/Survivin signaling pathway human keratinocyte |
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