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姜美泠, 陈杨, 刘向前.高效液相色谱法同时测定新变种太白白毛五加叶中5种苯丙素成分的含量[J].湖南中医药大学学报,2025,45(10):1869-1873[点击复制] |
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| 高效液相色谱法同时测定新变种太白白毛五加叶中5种苯丙素成分的含量 |
| 姜美泠,陈杨,刘向前 |
| (湖南中医药大学药学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 采用高效液相色谱（HPLC）同时检测新变种太白白毛五加叶中5种苯丙素类成分的含量，以期为该植物叶的质量标准建立提供参考。方法 运用高效液相色谱仪，采用Poroshell 120 EC-C18色谱柱（4.6 mm×250 mm，2.7 μm），流动相为0.1%磷酸（A）-乙腈（B），梯度洗脱，柱温30 ℃，流速1.0 mL/min，检测波长230 nm。结果 在优化的色谱条件下，5种成分均有良好的分离度和良好的线性关系，方法精密度RSD<2.0%，待测样品于室温24 h内能保持稳定，绿原酸、咖啡酸、4-香豆酸、阿魏酸、洒维宁线性范围分别为 0.175 0~1.750 0 μg（r1=0.999 2）、0.008 4~0.084 0 μg（r2=0.999 3）、0.002 4~0.024 0 μg（r3=0.999 1）、0.003 0~0.030 0 μg（r4=0.999 5）、0.026 0~0.260 0 μg（r5=0.999 1），平均加样回收率分别为99.93%（RSD=1.02%）、96.42%（RSD=2.32%）、99.17%（RSD=2.19%）、98.17%（RSD=1.71%）、99.03%（RSD=1.32%）（n=6）。结论 该方法准确、重现性好，专属性强，可为系统研究新变种太白白毛五加叶有效成分含量及其药用资源开发提供参考。 |
| 关键词: 新变种太白白毛五加叶 高效液相色谱 绿原酸 咖啡酸 4-香豆酸 阿魏酸 洒维宁 |
| DOI：10.3969/j.issn.1674-070X.2025.10.009 |
| 投稿时间：2025-07-05 |
| 基金项目:湖南省自然科学基金项目（2024JJ7357）；湖南中医药大学“十四五”重点学科——生物工程学科（校行发规字〔2023〕2号）。 |
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| Simultaneous determination of five phenylpropanoids in the leaves of Acanthopanax senticosus var. taibeiensis X.Q.Liu, S.K.Ko, J.Luo, et C.S.Yook, a new variety of Acanthopanax Miq. (Araliaceae) by HPLC |
| JIANG Meiling, CHEN Yang, LIU Xiangqian |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To simultaneously determine the content of five phenylpropanoids in the leaves of Acanthopanax senticosus var. taibeiensis X.Q.Liu, S.K.Ko, J.Luo, et C.S.Yook, a new variety of Acanthopanax Miq. (Araliaceae) using the HPLC method, aiming to provide a reference for establishing the quality standard of its leaves. Methods A HPLC system was used with a Poroshell 120 EC-C18 column (4.6 mm×250 mm, 2.7 μm). The mobile phase consisted of 0.1% phosphoric acid (A) and acetonitrile (B) with gradient elution. The column temperature was set at 30℃, the flow rate at 1.0 mL/min, and the detection wavelength at 230 nm. Results Under the optimized chromatographic conditions, all five components showed good resolution and linearity. The method demonstrated acceptable precision (RSD<2.0%), and the analytes remained stable at room temperature within 24 hours. The linear ranges of chlorogenic acid, caffeic acid, 4-coumaric acid, ferulic acid, and Savinin were 0.175 0-1.750 0 μg (r1=0.999 2), 0.008 4-0.084 0 μg (r2=0.999 3), 0.002 4-0.024 0 μg (r3=0.999 1), 0.003 0-0.030 0 μg (r4=0.999 5), and 0.026 0-0.260 0 μg (r5=0.999 1), respectively. The average spiked recovery rates (n=6) were 99.93% (RSD=1.02%), 96.42% (RSD=2.32%), 99.17% (RSD=2.19%), 98.17% (RSD=1.71%), and 99.03% (RSD=1.32%), respectively. Conclusion This method is stable, accurate, reproducible, and highly specific, providing a reference for the systematic research on the active ingredients in the leaves of Acanthopanax senticosus var. taibeiensis X.Q.Liu, S.K.Ko, J.Luo, et C.S.Yook and the development of its medicinal resources. |
| Key words: leaves of Acanthopanax senticosus var. taibeiensis X.Q.Liu, S.K.Ko, J.Luo, et C.S.Yook HPLC chlorogenic acid caffeic acid 4-coumaric acid ferulic acid Savinin |
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