| 引用本文: |
王梦云, 朱明芳, 李文姣, 李鹏宇, 石继连, 梁玲, 刘传义.UHPLC-Orbitrap-MS联合网络药理学分析杜仲雄花的化学成分及抗衰老作用机制[J].湖南中医药大学学报,2026,46(9):1825-1833[点击复制] |
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| UHPLC-Orbitrap-MS联合网络药理学分析杜仲雄花的化学成分及抗衰老作用机制 |
| 王梦云,朱明芳,李文姣,李鹏宇,石继连,梁玲,刘传义 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学, 湖南 长沙 410208;湖南神舟中药饮片有限公司, 湖南 张家界 427200) |
| 摘要: |
| 目的 采用超高效液相色谱耦合静电轨道阱高分辨质谱（UHPLC-Orbitrap-MS）技术和网络药理学方法探讨杜仲雄花的化学成分及其抗衰老作用机制。方法 通过UHPLC-Orbitrap-MS鉴定杜仲雄花的主要化学成分，结合SwissTargetPrediction数据库预测其相关靶点；利用GeneCards及OMIM数据库获取衰老相关靶点，与杜仲雄花化学成分靶点取交集，筛选出共同靶点；构建蛋白质-蛋白质相互作用网络（PPI），通过GO和KEGG富集分析，筛选杜仲雄花抗衰老的重要信号通路；通过ABTS与DPPH自由基清除实验，评价杜仲雄花抗氧化活性。结果 从杜仲雄花中鉴别出40个化学成分，共获得杜仲雄花化学成分与衰老的交集靶点187个，PPI网络得到159个节点，核心成分是槲皮素和柚皮素，其中槲皮素对ABTS和DPPH自由基表现出较强的体外清除活性，核心靶点包括肿瘤蛋白p53（p53）、信号转导与转录激活因子3（STAT3）、热激蛋白90α家族A类成员1（HSP90AA1）、SRC原癌基因非受体酪氨酸激酶（SRC）、蛋白激酶B1（Akt1）。GO富集分析和KEGG通路富集分析结果表明，杜仲雄花发挥抗衰老作用与磷脂酰肌醇3-激酶-Akt（PI3K-Akt）信号通路相关。结论 杜仲雄花中的化学成分可能通过多通路、多靶点协同调节细胞周期，减轻氧化应激损伤，抑制慢性炎症，维持细胞稳态，进而发挥延缓细胞衰老、改善机体衰老相关病理生理进程的作用。 |
| 关键词: UHPLC|杜仲雄花|抗衰老|网络药理学|PI3K-Akt |
| DOI：10.3969/j.issn.1674-070X.2026.09.010 |
| 投稿时间：2026-05-14 |
| 基金项目:湖南省芙蓉计划卫生健康高层次人才项目(湘卫函[2024]182号);湖南中医药大学和湖南省中医药民族医药国际联合实验室开放基金项目(2025ZYYKF11)。 |
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| Chemical constituents and anti-aging mechanisms of action of Eucommia ulmoides Oliv.male flowers based on UHPLC-Orbitrap-MS and network pharmacology |
| WANG Mengyun, ZHU Mingfang, LI Wenjiao, LI Pengyu, SHI Jilian, LIANG Ling, LIU Chuanyi |
| (The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Shenzhou Chinese Herbal Decoction Pieces Co., Ltd., Zhangjiajie, Hunan 427200, China) |
| Abstract: |
| Objective To explore the chemical constituents and anti-aging mechanisms of action of Eucommia ulmoides Oliv. male flowers using ultra-high performance liquid chromatography-orbitrap mass spectrometry (UHPLC-Orbitrap-MS) and network pharmacology. Methods The major chemical constituents of E. ulmoides male flowers were identified by UHPLC-Orbitrap-MS, and their related targets were predicted using the SwissTargetPrediction database. Aging-related targets were retrieved from the GeneCards and OMIM databases and intersected with the targets of the identified constituents of E. ulmoides male flowers to identify the common targets. A protein-protein interaction (PPI) network was constructed, and GO and KEGG enrichment analyses were performed to identify key signaling pathways involved in the anti-aging effects of E. ulmoides male flowers. The antioxidant activity of E. ulmoides male flowers was evaluated by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Results A total of 40 chemical constituents were identified from E. ulmoides male flowers, and 187 common targets were obtained between the chemical constituent-related targets and aging-related targets. The PPI network comprised 159 nodes. The core constituents were quercetin and naringenin, among which quercetin showed strong in vitro scavenging activity against ABTS and DPPH free radicals. The core targets included tumor protein p53 (TP53), signal transducer and activator of transcription 3 (STAT3), heat shock protein 90 alpha family class A member 1 (HSP90AA1), SRC proto-oncogene, non-receptor tyrosine kinase (SRC), and Akt serine/threonine kinase 1 (Akt1). GO and KEGG enrichment analyses indicated that the anti-aging effects of E. ulmoides male flowers were associated with the phosphatidylinositol 3-kinase/protein kinase B (PI3K-Akt) signaling pathway. Conclusion The chemical constituents in E. ulmoides male flowers may exert anti-aging effects by coordinately regulating the cell cycle through multiple pathways and targets, alleviating oxidative stress, inhibiting chronic inflammation, and maintaining cellular homeostasis, thereby delaying cellular senescence and ameliorating aging-related pathophysiological processes. |
| Key words: ultra-high performance liquid chromatography-Orbitrap mass spectrometry|Eucommia ulmoides Oliv.male flowers|anti-aging|network pharmacology|phosphatidylinositol 3-kinase/protein kinase B |
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