| 引用本文: |
谭若彤, 谢文彬, 杨尚林, 谢玮琪, 唐雨兰, 李铁浪.基于PGC-1α/NRF1信号通路探究点按脾俞穴对慢性疲劳综合征大鼠认知障碍的影响[J].湖南中医药大学学报,2025,45(12):2325-2334[点击复制] |
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| 基于PGC-1α/NRF1信号通路探究点按脾俞穴对慢性疲劳综合征大鼠认知障碍的影响 |
| 谭若彤,谢文彬,杨尚林,谢玮琪,唐雨兰,李铁浪 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 基于过氧化物酶体增殖受体γ辅激活因子-1α（PGC-1α）/核呼吸因子1（NRF1）通路探讨点按脾俞穴对慢性疲劳综合征（CFS）大鼠认知障碍的作用机制。方法 将32只大鼠随机分为空白组8只、造模组24只。造模组采用21 d多因素复合应激刺激法建立CFS大鼠模型，造模结束后，再将造模组大鼠随机分为模型组、点按组和人参皂苷组，每组8只。空白组和模型组予以1 mL/d的生理盐水腹腔注射；点按组点按双侧脾俞穴后，再予以1 mL/d的生理盐水腹腔注射；人参皂苷组予1 mL/d人参皂苷溶液腹腔注射。均1次/d，连续干预14 d。在造模前、造模后和干预后，进行大鼠体质量测量、一般情况半定量评分评估、力竭游泳实验和Morris水迷宫实验；干预后，采用比色法检测大鼠海马组织中腺苷三磷酸（ATP）含量，硫代巴比妥酸比色（TBA）法检测海马组织中丙二醛（MDA）含量，水溶性四唑盐（WST-1）法检测海马组织中超氧化物歧化酶（SOD）活性，实时荧光定量PCR法检测海马组织中PGC-1α、NRF1 mRNA表达量，Western blot检测海马组织中PGC-1α、NRF1蛋白表达水平。结果 （1）在造模前，各组大鼠的体质量、一般情况半定量评分、力竭游泳时间和Morris水迷宫实验相关指标的比较，差异无统计学意义（P>0.05）。在造模后，与空白组比较，模型组、点按组和人参皂苷组大鼠的体质量下降（P<0.01），一般情况半定量评分升高（P<0.01），力竭游泳时间缩短（P<0.05或P<0.01），Morris水迷宫实验的逃避潜伏期延长（P<0.05），运动总路程减少（P<0.05或P<0.01），穿越平台次数减少（P<0.01）；模型组、点按组和人参皂苷组大鼠之间的上述指标比较，差异无统计学意义（P>0.05）。在干预后，与空白组比较，模型组大鼠的体质量下降（P<0.01），一般情况半定量评分升高（P<0.01），力竭游泳时间缩短（P<0.05），Morris水迷宫实验的逃避潜伏期延长（P<0.05），运动总路程减少（P<0.01），穿越平台次数减少（P<0.01）；与模型组比较，点按组和人参皂苷组大鼠的体质量升高（P<0.01），一般情况半定量评分降低（P<0.01），力竭游泳时间延长（P<0.01），Morris水迷宫实验的逃避潜伏期缩短（P<0.01），运动总路程增加（P<0.01），穿越平台次数增加（P<0.05或P<0.01）；点按组和人参皂苷组大鼠之间的上述指标比较，差异无统计学意义（P>0.05）。（2）与空白组比较，模型组大鼠海马组织中ATP含量和SOD活性降低（P<0.05），MDA含量升高（P<0.05），PGC-1α、NRF1蛋白表达水平降低（P<0.01），PGC-1α、NRF1 mRNA表达量减少（P<0.01）。与模型组比较，点按组和人参皂苷组大鼠的海马组织中ATP含量和SOD活性升高（P<0.05），MDA含量降低（P<0.05），PGC-1α、NRF1蛋白表达水平升高（P<0.05或P<0.01），PGC-1α、NRF1 mRNA表达量升高（P<0.01）。结论 点按脾俞穴可以有效改善CFS大鼠疲劳状态和认知障碍，提高海马组织中ATP含量、增强SOD活性，降低MDA含量，其作用机制可能与激活PGC-1α/NRF1信号通路、改善线粒体能量代谢有关。 |
| 关键词: 慢性疲劳综合征 点按法 脾俞穴 过氧化物酶体增殖受体γ辅激活因子α/核呼吸因子1信号通路 线粒体能量代谢 认知障碍 |
| DOI：10.3969/j.issn.1674-070X.2025.12.011 |
| 投稿时间：2025-08-18 |
| 基金项目:湖南省自然科学基金面上项目（2023JJ30454）；湖南中医药大学大学生创新创业训练项目（S202410541038）。 |
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| Effects of acupressure at "Pishu (BL20)" on cognitive impairment in rats with chronic fatigue syndrome based on the PGC-1α/NRF1 signaling pathway |
| TAN Ruotong, XIE Wenbin, YANG Shanglin, XIE Weiqi, TANG Yulan, LI Tielang |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the mechanism of action of acupressure at "Pishu (BL20)" on cognitive impairment in rats with chronic fatigue syndrome (CFS) based on the peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α)/nuclear respiratory factor 1 (NRF1) pathway. Methods Thirty-two rats were randomly divided into blank group (n=8) and modeling group (n=24). The CFS model was established in the modeling group using a multifactorial compound stimulation method for a modeling period of 21 days. After modeling, rats in the modeling group were randomly divided into model group, "Pishu (BL20)" acupressure group, and ginsenoside group, with eight rats in each group. The blank group and model group received the intraperitoneal injection of physiological saline at 1 mL/day. The "Pishu (BL20)" acupressure group received bilateral acupressure at "Pishu (BL20)" followed by additional intraperitoneal injection of physiological saline at 1 mL/day. The ginsenoside group received intraperitoneal injection of ginsenoside solution at 1 mL/day. All interventions were administered once daily for 14 consecutive days. Assessments were conducted before and after modeling, and after intervention, including rats' body weight measurement, semi-quantitative scores of general condition, exhaustive swimming test and Morris water maze experiment. After the intervention, the adenosine triphosphate (ATP) content in the hippocampal tissue of rats was measured by colorimetry, the malondialdehyde (MDA) content in the hippocampal tissue was determined by thiobarbituric acid (TBA) colorimetry method, the superoxide dismutase (SOD) activity in the hippocampal tissue was tested by water-soluble tetrazolium salt 1 (WST-1) method, and the mRNA expressions of PGC-1α and NRF1 in the hippocampal tissue were measured by real-time fluorescence quantitative polymerase chain reaction (PCR). The protein expression levels of PGC-1α and NRF1 in the hippocampal tissue were determineded by Western blot. Results (1) Before modeling, there were no statistically significant differences in body weight, semi-quantitative scores of general condition, exhaustive swimming time, and related indicators of the Morris water maze experiment among different groups of rats (P>0.05). After modeling, compared with the blank group, rats in the model group, "Pishu (BL20)" acupressure group, and ginsenoside group all showed decreased body weight (P<0.01), increased semi-quantitative scores of general condition (P<0.01), shortened exhaustive swimming time (P<0.05 or P<0.01), prolonged escape latency in the Morris water maze experiment (P<0.05), decreased total distance traveled (P<0.05 or P<0.01) and number of platform crossings (P<0.01). There were no statistically significant differences in the above indicators among the model group, "Pishu (BL20)" acupressure group, and ginsenoside group (P>0.05). After intervention, compared with the blank group, rats in the model group exhibited decreased body weight (P<0.01), increased semi-quantitative scores of general condition (P<0.01), shortened exhaustive swimming time (P<0.05), prolonged escape latency in the Morris water maze experiment (P<0.05), decreased total distance traveled (P<0.01) and number of platform crossings (P<0.01). Compared with the model group, rats in the "Pishu (BL20)" acupressure group and ginsenoside group both showed increased body weight (P<0.01), decreased semi-quantitative scores of general condition (P<0.01), and shortened exhaustive swimming time (P<0.01), shortened escape latency in the Morris water maze experiment (P<0.01), increased total distance traveled (P<0.01), and number of platform crossings (P<0.05 or P<0.01). There were no statistically significant differences in the above indicators between the "Pishu (BL20)" acupressure group and ginsenoside group (P>0.05). (2) Compared with the blank group, rats in the model group showed decreased ATP content and SOD activity in the hippocampal tissue (P<0.05), increased MDA content (P<0.05), decreased protein expression levels of PGC-1α and NRF1 (P<0.01) and mRNA expression levels of PGC-1α and NRF1 (P<0.01). Compared with the model group, rats in the "Pishu (BL20)" acupressure group and ginsenoside group both showed increased ATP content and SOD activity in the hippocampal tissue (P<0.05), and decreased MDA content (P<0.05), increased protein expression levels of PGC-1α and NRF1 (P<0.05 or P<0.01) and mRNA expression levels of PGC-1α and NRF1 (P<0.01). Conclusion Acupressure at "Pishu (BL20)" can effectively ameliorate the fatigue state and cognitive impairment in rats with CFS. It increases ATP content and SOD activity while reducing MDA content in the hippocampal tissue. The mechanism of action may be related to the activation of the PGC-1α/NRF1 signaling pathway and the improvement of mitochondrial energy metabolism. |
| Key words: chronic fatigue syndrome acupressure "Pishu (BL20)" PGC-1α/NRF1 signaling pathway mitochondrial energy metabolism cognitive impairment |
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