| 引用本文: |
赖菁菁, 李美康, 韩海涛, 李婕, 黄瑾明, 谭磊, 徐晶, 罗红.壮医莲花针拔罐逐瘀法对带状疱疹后神经痛小鼠β-catenin、FZ8及炎症因子的影响[J].湖南中医药大学学报,2025,45(6):1037-1043[点击复制] |
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| 壮医莲花针拔罐逐瘀法对带状疱疹后神经痛小鼠β-catenin、FZ8及炎症因子的影响 |
| 赖菁菁,李美康,韩海涛,李婕,黄瑾明,谭磊,徐晶,罗红 |
| (广西中医药大学第一附属医院壮医针灸科, 广西 南宁 530023) |
| 摘要: |
| 目的 探讨壮医莲花针拔罐逐瘀法对带状疱疹后神经痛（PHN）小鼠行为学变化及其对β-连环蛋白（β-catenin）、Frizzled-8（FZ8）、炎症因子的影响。方法 将40只KM小鼠随机分为空白对照组（Control组）、带状疱疹后神经痛组（PHN组）、加巴喷丁组（JBPD组）、壮医莲花针拔罐逐瘀组（ZY组），每组10只。采用树脂毒素制作PHN小鼠模型，观察各组小鼠干预后行为学检测机械缩足反射阈值（PWT）和热缩足反射潜伏期（TWL）的变化，采用ELISA检测小鼠血清中白细胞介素-18（IL-18）、肿瘤坏死因子-α（TNF-α）的含量，采用免疫组化及Western blot法检测脊髓后角β-catenin、FZ8因子的表达。结果 造模后，与Control组相比，PHN组PWT降低、TWL升高（P＜0.05）,提示模型构建成功。与PHN组相比，JBPD组和ZY组在治疗第10天开始PWT升高（P＜0.05），在治疗第15天开始TWL降低（P＜0.05）；与JBPD组相比，ZY组在治疗第25天PWT值升高、TWL降低（P＜0.05）。与Control组相比，PHN组IL-18、TNF-α表达升高（P＜0.05）；与PHN组相比，JBPD组和ZY组IL-18、TNF-α表达降低（P＜0.05）。与Control组相比，PHN组β-catenin、FZ8表达升高（P＜0.05）；与PHN组相比，JBPD组与ZY组的β-catenin、FZ8表达降低（P＜0.05）。结论 壮医莲花针拔罐逐瘀法对PHN的镇痛机制可能是其通过抑制β-catenin信号通路，降低炎症因子IL-18、TNF-α的表达，从而减轻炎症反应，达到镇痛的目的。 |
| 关键词: 带状疱疹后神经痛 壮医莲花针拔罐逐瘀法 白细胞介素-18 肿瘤坏死因子-α β-连环蛋白 Frizzled-8 |
| DOI：10.3969/j.issn.1674-070X.2025.06.007 |
| 投稿时间：2024-12-04 |
| 基金项目:国家自然科学基金项目（82060904）；广西壮族自治区中管局壮医学重点学科项目（GZXK-Z-20-59）；广西黄氏壮医针灸流派传承工作室建设项目[国中医药人教函〔2019〕62号]；广西名中医李美康传承工作室建设项目[桂中医药科教发〔2024〕1号]。 |
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| Effects of Zhuang medicine Lotus Needle Cupping Stasis-Resolving Method on β-catenin, FZ8, and inflammatory factors in mice with postherpetic neuralgia |
| LAI Jingjing, LI Meikang, HAN Haitao, LI Jie, HUANG Jinming, TAN Lei, XU Jing, LUO Hong |
| (Department of Zhuang Medicine Acupuncture, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, China) |
| Abstract: |
| Objective To investigate the effects of the Zhuang medicine Lotus Needle Cupping Stasis-Resolving Method on behavioral changes and the expressions of β-catenin, Frizzled-8 (FZ8) and inflammatory factors in mice with postherpetic neuralgia (PHN).Method Forty KM mice were randomly divided into a blank control group (Control group), a postherpetic neuralgia group (PHN group), a gabapentin group (JBPD group), and a Zhuang medicine Lotus Needle Cupping and Stasis-Resolving group (ZY group), with 10 mice in each group. The PHN mouse model was established using resiniferatoxin. Behavioral changes, including paw withdrawal threshold (PWT) and thermal withdrawal latency (TWL), were observed post-intervention in each group of mice. ELISA was used to measure the levels of interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) in mouse serum, and immunohistochemistry and Western blot were used to measure the expressions of β-catenin and FZ8 factors in the spinal cord posterior horn. Result After modeling, compared with the Control group, the PHN group showed decreased PWT and increased TWL (P<0.05), indicating successful model establishment. Compared with the PHN group, both JBPD and ZY groups showed increased PWT on the 10th day of treatment (P<0.05) and decreased TWL on the 15th day of treatment (P<0.05). Compared with the JBPD group, the ZY group showed increased PWT and decreased TWL on the 25th day of treatment (P<0.05). Compared with the Control group, the expressions of IL-18 and TNF-α increased in the PHN group (P<0.05). Compared with the PHN group, the expressions of IL-18 and TNF-α decreased in the JBPD and ZY groups (P<0.05). Compared with the Control group, the expressions of β-catenin and FZ8 increased in the PHN group (P<0.05). Compared with the PHN group, the expressions of β-catenin and FZ8 decreased in the JBPD and ZY group (P<0.05).Conclusion The analgesic mechanism of the Zhuang medicine Lotus Needle Cupping and Stasis-Resolving Method for PHN may be achieved by inhibiting the β-catenin signaling pathway, reducing the expressions of inflammatory factors IL-18 and TNF-α, thereby alleviating the inflammatory response and achieving the goal of analgesia. |
| Key words: postherpetic neuralgia Zhuang medicine Lotus Needle Cupping Stasis-Resolving Method interleukin-18 tumor necrosis factor-α β-catenin Frizzled-8 |
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