| 引用本文: |
潘杰灵, 唐丽亚, 张玉乔, 闪逸仙, 李武, 李江山.机械敏感性离子通道Piezo2在激痛点炎症诱导机械痛觉过敏中的潜在作用[J].湖南中医药大学学报,2025,45(5):869-876[点击复制] |
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| 机械敏感性离子通道Piezo2在激痛点炎症诱导机械痛觉过敏中的潜在作用 |
| 潘杰灵,唐丽亚,张玉乔,闪逸仙,李武,李江山 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208;澳门科技大学中医药学院, 澳门 999078) |
| 摘要: |
| 目的 观察机械敏感性离子通道Piezo2在激痛点炎症诱导的机械痛觉过敏中的作用。方法 将30只SPF级雄性SD大鼠随机分为空白组、模型组、弗氏佐剂组、依托考昔组及Piezo2抑制剂组（以下简称抑制剂组），每组6只。除空白组外，其余4组大鼠均造模，造模部位为左侧股内侧肌。造模完成后，在弗氏佐剂组大鼠激痛点局部注射150 μL弗氏完全佐剂（CFA）1次；依托考昔组大鼠以5.5 mg/kg剂量的依托考昔悬浊液灌胃，每日2次，连续灌胃2 d；抑制剂组大鼠予激痛点组织注射60 μg/μL抑制剂1次，注射量为120 μL。干预前后，检测激痛点局部机械痛阈值和软组织张力。干预2周后收集激痛点样本，HE染色观察其显微结构，ELISA法检测白细胞介素-1β（IL-1β）含量，免疫组织化学法和Western blot法检测Piezo2的表达。结果 与干预前比较，干预后弗氏佐剂组机械痛阈值、D0.2 kg 值均降低（P＜0.05，P＜0.01），依托考昔组和抑制剂组均升高（P＜0.05，P＜0.01）。干预后，与空白组比较，模型组机械痛阈值、D0.2 kg 值均降低（P＜0.01）；与模型组比较，弗氏佐剂组机械痛阈值、D0.2 kg 值均降低（P＜0.01），依托考昔组和抑制剂组均升高（P＜0.01）；与弗氏佐剂组比较，依托考昔组和抑制剂组机械痛阈值、D0.2 kg 值均升高（P＜0.01）。HE染色显示：模型组和弗氏佐剂组肌细胞受损，局部炎性细胞浸润；依托考昔组和抑制剂组肌细胞受损明显减轻，局部炎症细胞减少。与空白组比较，模型组IL-1β含量升高（P＜0.01）；与模型组比较，弗氏佐剂组IL-1β 含量升高（P＜0.01），依托考昔组和抑制剂组均降低（P＜0.01）；与弗氏佐剂组比较，依托考昔组和抑制剂组IL-1β含量均降低（P＜0.01）。与空白组比较，模型组Piezo2表达升高（P＜0.01）；与模型组比较，依托考昔组和抑制剂组Piezo2表达均降低（P＜0.05），弗氏佐剂组差异无统计学意义（P＞0.05）；与弗氏佐剂组比较，依托考昔组和抑制剂组Piezo2表达均降低（P＜0.05，P＜0.01）。结论 （1）弗氏完全佐剂能增强激痛点局部炎症反应、肌细胞受损，依托考昔和Piezo2抑制剂可抑制其炎症反应；（2）Piezo2可能在激痛点组织炎症所致机械痛觉过敏中发挥作用。 |
| 关键词: 激痛点 Piezo2 炎症反应 外周敏化 机械痛觉过敏 弗氏完全佐剂 依托考昔 |
| DOI：10.3969/j.issn.1674-070X.2025.05.012 |
| 投稿时间：2024-12-03 |
| 基金项目:国家自然科学基金面上项目（82174526，82274676，82374613，82474669）；湖南省科技创新计划资助项目（2022RC1221）。 |
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| The potential role of mechanosensitive Piezo2 ion channels in myofascial trigger point inflammation-induced mechanical hyperalgesia |
| PAN Jieling, TANG Liya, ZHANG Yuqiao, SHAN Yixian, LI Wu, LI Jiangshan |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Faculty of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China) |
| Abstract: |
| Objective To observe the effects of Piezo2 on mechanical hyperalgesia induced by myofascial trigger point inflammation. Methods Thirty SPF-grade male SD rats were randomly divided into blank group, model group, complete Freund's adjuvant (CFA) group, etoricoxib group, and Piezo2 inhibitor group (hereinafter referred to as the inhibitor group), with six rats in each group. Except for the blank group, models were established in the other four groups at the modeling site of the left vastus medialis muscle. After modeling, the rats in the CFA group received a single 150 uL CFA injection at the trigger point. The etoricoxib group was administered 5.5 mg/kg etoricoxib suspension via gavage twice daily for two consecutive days. The inhibitor group received a single 120 μL injection of Piezo2 inhibitor (60 μg/μL) at the trigger point tissue. Mechanical pain threshold and soft tissue tension were measured before and after intervention. After two weeks of intervention, trigger point samples were collected for microscopic structural observation by HE staining, measurement of interleukin-1β (IL-1β) levels via ELISA, and analysis of Piezo2 expression using both immunohistochemistry and Western blot. Results Compared with before intervention, the mechanical pain threshold and D 0.2kg value in the CFA group decreased after intervention (P<0.05, P<0.01), while those in the etoricoxib group and the inhibitor group increased (P<0.05, P<0.01). After intervention, compared with the blank group, the model group showed a decrease in mechanical pain threshold and D 0.2kg value (P<0.01); compared with the model group, the mechanical pain threshold and D 0.2kg value in the CFA group decreased (P<0.01), while those in the etoricoxib group and the inhibitor group increased (P<0.01); compared with the CFA group, the mechanical pain threshold and D 0.2kg value increased in both the etoricoxib group and the inhibitor group (P<0.01). HE staining showed that muscle cells were damaged in the model group and CFA group, with local inflammatory cell infiltration; muscle cell damage was significantly alleviated and local inflammatory cells decreased in both the etoricoxib group and the inhibitor group. Compared with the blank group, the IL-1β content in the model group increased (P<0.01); compared with the model group, the content of IL-1β increased in the CFA group (P<0.01), while those in the etoricoxib group and the inhibitor group decreased (P<0.01); compared with the CFA group, the levels of IL-1β decreased in both the etoricoxib group and the inhibitor group (P<0.01). Compared with the blank group, the model group showed an increase in Piezo2 expression (P<0.01); compared with the model group, the expression of Piezo2 decreased in both the etoricoxib group and the inhibitor group (P<0.05), and there was no statistically significant difference in the CFA group (P＞0.05); Compared with the CFA group, the expression of Piezo2 decreased in both the etoricoxib group and the inhibitor group (P<0.05, P<0.01). Conclusion (1) CFA can enhance local inflammatory reaction and muscle cell damage in MTrPs, while etoricoxib and the Piezo2 inhibitor can inhibit the inflammatory reaction. (2) Piezo2 may play a role in mechanical hyperalgesia resulted from inflammation in MTrP tissue. |
| Key words: myofascial trigger point Piezo2 inflammatory reaction peripheral sensitization mechanical hyperalgesia Freund's complete adjuvant etoricoxib |
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