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陆文洪, 刘丽芳, 王真权, 罗雯鹏.矾冰纳米乳调节Wnt/β-catenin通路对糖尿病型肛瘘术后创面愈合的影响[J].湖南中医药大学学报,2025,45(5):810-817[点击复制] |
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| 矾冰纳米乳调节Wnt/β-catenin通路对糖尿病型肛瘘术后创面愈合的影响 |
| 陆文洪,刘丽芳,王真权,罗雯鹏 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学第一附属医院, 湖南 长沙 410021) |
| 摘要: |
| 目的 观察矾冰纳米乳对糖尿病型肛瘘术后大鼠创面愈合及Wnt/β-连环蛋白（β-catenin）信号通路相关蛋白表达的影响，探讨其促进创面修复的作用机制。方法 将72只SPF级SD雌性大鼠随机分为假手术组、模型组、矾冰纳米乳组和湿润烧伤膏组（每组18只），均建立糖尿病大鼠模型。除假手术组外，其余各组再建立糖尿病肛瘘术后创面模型。模型组每天予局部覆盖生理盐水纱条，矾冰纳米乳组每天予局部覆盖矾冰纳米乳纱条，湿润烧伤膏组每天予局部覆盖湿润烧伤膏纱条，连续干预21 d。分别于第7、14、21天观察各组大鼠体质量、精神状态、活动能力及创面愈合率；HE染色检测创面组织病理变化及毛细血管计数；Western blot检测创面组织中Wnt1、β-catenin、细胞周期蛋白D1（Cyclin D1）、癌基因C-myc（C-myc）、血管内皮生长因子（VEGF）、血管内皮生长因子受体2（VEGFR2）及糖原合酶激酶3β（GSK-3β）蛋白表达水平。结果 与假手术组比较，模型组大鼠体质量、创面愈合率降低（P＜0.05）；组织炎症浸润增加，毛细血管计数减少（P＜0.05）；第21天Wnt1、β-catenin、Cyclin D1、C-myc蛋白表达水平升高（P＜0.05，P＜0.01），VEGF、VEGFR2和GSK-3β表达水平下降（P＜0.01）。与模型组比较，矾冰纳米乳组与湿润烧伤膏组大鼠体质量、创面愈合率上升（P＜0.01）；炎症细胞减少、毛细血管计数增加（P＜0.05）；第21天Wnt1、β-catenin、C-myc、Cyclin D1、VEGF与VEGFR2蛋白表达水平升高（P＜0.05，P＜0.01），GSK-3β表达水平下降（P＜0.01）。结论 矾冰纳米乳可能通过激活Wnt/β-catenin通路的相关蛋白，从而改善创面组织病理状态，促进创面愈合。 |
| 关键词: 矾冰纳米乳 Wnt/β-catenin信号通路 糖尿病 肛瘘 术后创面 |
| DOI：10.3969/j.issn.1674-070X.2025.05.005 |
| 投稿时间：2024-12-25 |
| 基金项目:湖南省中医药管理局一般资助项目（B2023149）；湖南省卫生健康委员会卫生科研课题一般资助项目（B202304136861）；湖南省教育厅优秀青年项目（24B0360）；湖南中医药大学研究生创新课题（2024CX009）；湖南中医药大学校院联合基金教学改革研究重点项目（2022-LHJG003）。 |
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| Effects of alum-ice nanoemulsion on wound healing after surgery for diabetic anal fistula by regulating Wnt/β-Catenin pathway |
| LU Wenhong, LIU Lifang, WANG Zhenquan, LUO Wenpeng |
| (The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effects of alum-ice nanoemulsion on postoperative wound healing in diabetic rats with anal fistula and the expression of proteins related to the Wnt/β-catenin signaling pathway in rats after surgery for diabetic anal fistula, and to explore its mechanism of action in promoting wound repair. Methods Seventy-two SPF-grade female SD rats were randomly divided into sham-operated group, model group, alum-ice nanoemulsion group, and Moist Burn Ointment (MBO) group, with 18 rats in each group. A diabetic rat model was established in all groups. Subsequently, a postoperative wound model of diabetic anal fistula was created in all groups except the sham-operated group. The model group received daily local coverage with saline-soaked gauze, the nanoemulsion group received alum-ice nanoemulsion-soaked gauze, and the MBO group received MBO-soaked gauze. The treatments were applied for 21 consecutive days. The body weight, mental status, activity ability, and wound healing rate of the rats in each group were observed on the 7th, 14th, and 21st days respectively. HE staining was used to examine the pathological changes and capillary density in the wound tissue. Western blot was employed to check the expression levels of Wnt1, β-catenin, Cyclin D1, C-myc, vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), and glycogen synthase kinase 3β (GSK-3β) proteins in wound tissue. Results Compared with the sham-operated group, rats in the model group showed decreased body weight and wound healing rate (P<0.05), increased inflammatory cell infiltration, and reduced capillary count in the wound tissue (P<0.05). On day 21, the expression levels of Wnt1, β-catenin, Cyclin D1, and C-myc proteins were significantly elevated (P<0.05, P<0.01), while the expression levels of VEGF, VEGFR2, and GSK-3β significantly decreased (P<0.01). Compared with the model group, both the alum-ice nanoemulsion group and the MBO group showed increased body weight and wound healing rate (P<0.05), reduced inflammatory cell infiltration, and increased capillary count (P<0.05). On day 21, the expression levels of Wnt1, β-catenin, Cyclin D1, C-myc, VEGF, and VEGFR2 proteins were significantly upregulated (P<0.05, P<0.01), while GSK-3β expression was significantly downregulated (P<0.01). Conclusion Alum-ice nanoemulsion may promote wound healing by activating proteins related to the Wnt/β-catenin signaling pathway, thereby improving the histopathological condition of the wound tissue. |
| Key words: alum-ice nanoemulsion Wnt/β-catenin signaling pathway diabetes anal fistula postoperative wound |
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