| 引用本文: |
李祖强, 丁攀婷, 梁枝懿, 周竞颖, 刘琼, 钟欢, 刘密.电针内关、膻中穴改善慢性心力衰竭模型大鼠的症状及代谢组学特征[J].湖南中医药大学学报,2025,45(1):65-72[点击复制] |
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| 电针内关、膻中穴改善慢性心力衰竭模型大鼠的症状及代谢组学特征 |
| 李祖强,丁攀婷,梁枝懿,周竞颖,刘琼,钟欢,刘密 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨电针内关、膻中穴对慢性心力衰竭（CHF）大鼠症状及代谢组学特征的作用。方法 SD大鼠经7 d适应性喂养后，采用腹腔隔日注射多柔比星（DOX）诱导建立CHF模型。采用随机数字表法将造模成功的CHF大鼠随机分为模型组（n=11）和电针组（n=14），另设置空白对照组（n=11），共3组。电针组大鼠接受内关、膻中穴电针干预，每日1次，持续2周；模型组与空白对照组不做干预，仅进行常规束缚。干预结束后，通过超声心动图评估各组大鼠的心脏泵血功能、HE染色评估各组大鼠左心室心肌组织的病理变化。采用LC-MS检测各组大鼠的心肌组织代谢物，通过对不同组之间的代谢物含量的变化进行主成分分析、偏最小二乘法判别分析，筛选出差异代谢物，使用Metabo Analyst进行代谢通路分析。结果 与空白对照组相比，模型组超声心动图左心室射血分数（LVEF）、左心室短轴缩短率（LVFS）明显降低（P<0.01）。电针治疗后，与模型组相比，电针组的超声心动图LVEF、LVFS明显升高（P<0.01），说明电针可有效改善CHF大鼠心脏泵血功能。与模型组相比，电针干预后的心肌组织炎症浸润减少、平滑肌更加平滑，细胞结构趋向完整，表明电针可逆转CHF造成的心肌损伤。代谢组学检测中，与空白对照组相比，模型组心肌组织中有3种代谢物水平发生变化（P<0.05）,4条代谢通路发生改变。电针治疗后，与模型组相比，电针组有4种代谢物水平发生变化（P<0.05），其中1种代谢物出现明显回调；代谢通路分析表明，电针可调节嘌呤代谢、叶酸合成代谢和谷胱甘肽代谢3条通路。结论 电针内关、膻中穴对CHF大鼠的心脏泵血功能具有显著的改善作用，其机制可能与调控心肌能量代谢、氧化应激及核酸合成有关。 |
| 关键词: 慢性心力衰竭 电针 内关 膻中 代谢组学 超声心动图 |
| DOI：10.3969/j.issn.1674-070X.2025.01.009 |
| 投稿时间：2024-09-23 |
| 基金项目:国家自然科学基金项目(81904097);湖南省重点研发计划项目(2024JK2132);国家中医药管理局2022年青年岐黄学者培养项目(国中医药人教函[2022]256号);湖南省自然科学基金项目(2020JJ5430);湖南省中医药管理局项目(B2023109)。 |
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| On the improvements of electroacupuncture at "Neiguan"（PC6） and "Danzhong"（CV17） acupoints on symptoms and metabolomic characteristics in a rat model of chronic heart failure |
| LI Zuqiang, DING Panting, LIANG Zhiyi, ZHOU Jingying, LIU Qiong, ZHONG Huan, LIU Mi |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effects of electroacupuncture（EA） at "Neiguan"（PC6） and "Danzhong"（CV17） acupoints on symptoms and metabolomic characteristics in rats with chronic heart failure（CHF）. Methods After seven days of adaptive feeding,a CHF model was established in SD rats by intraperitoneal injections of doxorubicin（DOX） on alternate days. The successfully modeled CHF rats were randomized using a random number table into model group（n=11）, EA group（n=14）, and blank control group（n=11） Rats in the EA group received EA intervention at "Neiguan"（PC6） and "Danzhong"（CV17） acupoints once a day for two weeks, while the model and blank control groups only underwent routine restraint without intervention. At the end of the intervention, cardiac pumping function was evaluated by echocardiography, and pathological changes in left ventricular myocardial tissue of the rats in each group were assessed using H＆E staining. LC-MS was used to check the metabolites in myocardial tissue of rats in each group. Principal component analysis（PCA） and partial least squares-discriminant analysis（PLS-DA） were conducted to screen out differential metabolites. Metabolic pathway analysis was performed using Metabo Analyst. Results Compared with the blank control group, the left ventricular ejection fraction（LVEF） and left ventricular fractional shortening（LVFS） in the model group significantly decreased（P＜0.01）. After EA treatment, the LVEF and LVFS of echocardiogram in EA group were significantly increased compared with those in the model group（P＜0.01）, indicating that EA can effectively improve the cardiac pumping function of CHF rats. Compared to the model group, the myocardial tissues showed reduced inflammatory infiltration, smoother muscle fibers, and more intact cell structures after intervention in the EA group, suggesting that EA can reverse myocardial damage caused by CHF. In the metabolomic test, compared with the blank control group, the model group revealed changes in three metabolites（P＜0.05） and four metabolic pathways in the myocardial tissues. After EA treatment, four metabolites showed changes（P＜0.05）, with one metabolite significantly reversed. Metabolic pathway analysis indicated that EA can modulate purine metabolism, folate biosynthesis, and glutathione metabolism pathways. Conclusion EA at "Neiguan"（PC6） and "Danzhong"（CV17） acupoints significantly improves cardiac pumping function in CHF rats. The mechanism may be related to the regulation of myocardial energy metabolism, oxidative stress, and nucleotide synthesis. |
| Key words: chronic heart failure electroacupuntcture "Neiguan"（PC6） "Danzhong"（CV17） metabolomics echocardiography |
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