| 引用本文: |
田艳鹏, 陈卫卫, 王海峰, 赵泽鹏, 乔柱, 刘善军.基于Keap1/Nrf2信号通路探讨旋覆代赭汤含药血清对食管癌细胞的调控作用[J].湖南中医药大学学报,2025,45(1):30-38[点击复制] |
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| 基于Keap1/Nrf2信号通路探讨旋覆代赭汤含药血清对食管癌细胞的调控作用 |
| 田艳鹏,陈卫卫,王海峰,赵泽鹏,乔柱,刘善军 |
| (济宁医学院附属医院, 山东 济宁 272029) |
| 摘要: |
| 目的 探讨旋覆代赭汤含药血清对食管癌细胞的调控作用。方法 原代培养人食管癌TE-1、TE-10细胞7 d后，以不同浓度的旋覆代赭汤含药血清（0%、5%、10%、15%、20%、25%、30%）和不同浓度的多西他赛（0、0.1、1、5、10、50 nmol/L）处理细胞48 h。通过CCK-8实验筛选旋覆代赭汤含药血清最佳给药浓度。将TE-1细胞和TE-10细胞随机分为模型组、空白血清组、含药血清组和多西他赛组，分组处理后采用CCK-8实验检测细胞增殖能力；Transwell实验检测细胞迁移及侵袭能力；流式细胞术和Tunnel法检测细胞凋亡情况；免疫荧光检测Kelch样ECH相关蛋白1(Keap1)、核转录因子红系2相关因子2(Nrf2)蛋白表达水平。结果 旋覆代赭汤含药血清最佳给药浓度为30%、多西他赛最佳给药浓度为1 nmol/L。与模型组和空白血清组比较，含药血清组与多西他赛组TE-1、TE-10细胞的增殖、迁移、侵袭能力以及Keap1、Nrf2蛋白表达均降低（P<0.05），细胞凋亡率均升高（P<0.05）；与多西他赛组比较，含药血清组TE-1、TE-10细胞的增殖能力，TE-1细胞Keap1蛋白以及TE-10细胞Keap1、Nrf2蛋白表达均升高（P<0.05）,TE-1细胞的凋亡率降低（P<0.05）。结论 旋覆代赭汤含药血清可能通过调控Keap1/Nrf2信号通路抑制食管癌细胞的增殖。 |
| 关键词: 食管癌 旋覆代赭汤 多西他赛 Keap1/Nrf2通路 增殖 凋亡 |
| DOI：10.3969/j.issn.1674-070X.2025.01.005 |
| 投稿时间：2024-07-07 |
| 基金项目:济宁市重点研发计划项目(2023YXNS256);济宁医学院附属医院博士专项科研基金项目(2021-BS-020)。 |
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| Regulatory effects of Xuanfu Daizhe Decoction-medicated serum on esophageal cancer cells based on Keap1/Nrf2 signaling pathway |
| TIAN Yanpeng, CHEN Weiwei, WANG Haifeng, ZHAO Zepeng, QIAO Zhu, LIU Shanjun |
| (Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China) |
| Abstract: |
| Objective To investigate the regulatory effects of Xuanfu Daizhe Decoction（XFDZD）-medicated serum on esophageal cancer cells. Methods Human esophageal cancer TE-1 and TE-10 cells were subjected to primary culture for seven days, followed by treatment with different concentrations of XFDZD-medicated serum（0%, 5%, 10%, 15%, 20%, 25%, and 30%） and different concentrations of polyene paclitaxel（0, 0.1, 1, 5, 10, and 50 nmol/L） for 48 hours. The CCK-8 experiment was used to screen the optimal administration concentration of XFDZD-medicated serum. TE-1 and TE-10 cells were randomly divided into model, blank serum, medicated serum, and polyene paclitaxel groups. After grouping, the CCK-8 assay was used to determine cell proliferation ability. The Transwell assay was used to measure cell migration and invasion abilities. Flow cytometry and Tunnel assay were conducted to examine cell apoptosis, and immunofluorescence was used to determine the protein expression levels of Kelch-like ECH-associated protein 1（Keap1） and nuclear factor-erythroid 2-related factor 2（Nrf2）. Results The optimal administration concentration of XFDZD-medicated serum was 30%, and the optimal concentration of polyene paclitaxel was 1 nmol/L. Compared with the model group and the blank serum group, the proliferation, migration, invasion abilities, and the protein expression levels of Keap1 and Nrf2 of TE-1 and TE-10 cells were reduced in both the medicated serum group and the polyene paclitaxel group（P＜0.05）, while the cell apoptosis rates were elevated（P＜0.05）. Compared with polyene paclitaxel group, the proliferation ability of TE-1and TE-10 cells, the protein expression of Keap1 in TE-1 cells, and the protein expressions of Keap1 and Nrf2 in TE-10 cells were elevated in the medicated serum group（P＜0.05）, whereas the apoptosis rates of TE-1 cells was reduced（P＜0.05）. Conclusion XFDZD-medicated serum may inhibit the proliferation of esophageal cancer cells by regulating the Keap1/Nrf2 signaling pathway. |
| Key words: esophageal cancer Xuanfu Daizhe Decoction polyene paclitaxel Keap1/Nrf2 pathway proliferation apoptosis |
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