| 引用本文: |
谢思健, 马小丫, 唐逸飞, 杜佳琪, 冯娴, 赖娟, 伍竟涛, 姜尚萍, 彭清华.润目汤熏蒸对水液缺乏型干眼兔泪液分泌、角膜泪腺形态及炎症相关蛋白表达水平的影响[J].湖南中医药大学学报,2024,44(11):1991-1998[点击复制] |
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| 润目汤熏蒸对水液缺乏型干眼兔泪液分泌、角膜泪腺形态及炎症相关蛋白表达水平的影响 |
| 谢思健,马小丫,唐逸飞,杜佳琪,冯娴,赖娟,伍竟涛,姜尚萍,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208;西安交通大学, 陕西 西安 710049;中南大学湘雅三医院, 湖南 长沙 410013;厦门大学医学院, 福建 厦门 361023;宁乡市中医医院, 湖南 长沙 410000) |
| 摘要: |
| 目的 观察润目汤中药熏蒸对水液缺乏型干眼兔泪液分泌、角膜泪腺形态及炎症相关蛋白表达水平的影响。方法 取健康新西兰大白兔30只，随机分为5组（空白对照组、蒸馏水组、阳性对照组、联合治疗组和中药熏蒸组），每组6只。空白对照组不予处理，其余各组用1%硫酸阿托品眼用凝胶连续滴眼3 d。造模成功后（实验第4天开始），空白对照组在正常环境中常规喂养。蒸馏水组给予中药熏蒸治疗仪加热蒸馏水熏蒸眼部对照处理；阳性对照组予以玻璃酸钠滴眼液滴眼，1次1滴，4次/d；联合治疗组采用润目汤熏蒸和玻璃酸钠滴眼液联合滴眼治疗；中药熏蒸组采用中药熏蒸治疗仪加热润目汤水煎液熏蒸眼部，1次/d，15 min/次。同时各组均滴1%硫酸阿托品滴眼液以维持模型，直至实验结束。在实验第0、3、7、10、13天分别进行泪液分泌试验（Schirmer Ⅰ test, SⅠT）、角膜荧光素钠染色（corneal fluorescein staining, CFS）评分；第3、13天进行角膜共聚焦显微镜检查；第13天处死动物。在光镜下观察角膜上皮形态学变化，电镜下观察角膜上皮及泪腺组织形态学变化；采用Western blot检测角膜组织中白细胞介素-1α（interleukin-1α, IL-1α）、肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）的表达。结果 与空白对照组比较，在第7、10天，蒸馏水组和联合治疗组SⅠT降低（P<0.01），各组CFS评分升高（P<0.05）；在第13天，蒸馏水组SⅠT降低（P<0.01），蒸馏水组和阳性对照组CFS评分升高（P<0.05）。与蒸馏水组比较，在第7天，阳性对照组和中药熏蒸组SⅠT升高（P<0.01），联合治疗组CFS评分降低（P<0.05）；在第10、13天，阳性对照组、中药熏蒸组SⅠT升高（P<0.01）、CFS评分降低（P<0.01）。与阳性对照组比较，在第7天，联合治疗组SⅠT降低（P<0.05）；在第10天，联合治疗组CFS升高（P<0.05）。与联合治疗组比较，在第7、10天，中药熏蒸组SⅠT升高（P<0.05）。与空白对照组比较，阳性对照组、中药熏蒸组和联合治疗组兔角膜前基质神经密度有所恢复，细胞形态也接近空白对照组；未见明显的角膜上皮组织损伤，基底细胞排列整齐，形态与空白对照组接近。联合治疗组泪腺上皮细胞胞浆分泌丰富、角膜完整有序，最接近于空白对照组。与空白对照组比较，蒸馏水组IL-1α和TNF-α表达量升高（P<0.01）;与蒸馏水组比较，阳性对照组、中药熏蒸组和联合治疗组兔角膜中IL-1α表达量下降（P<0.01），中药熏蒸组和联合治疗组TNF-α表达量下降（P<0.01）。与阳性对照组比较，中药熏蒸组和联合治疗组TNF-α表达量下降（P<0.05）。结论 润目汤熏蒸可促进泪液分泌，减少角膜上皮与泪腺组织损伤，降低IL-1α、TNF-α表达，抑制局部炎症反应。 |
| 关键词: 润目汤 中药熏蒸 水液缺乏型干眼 炎症因子 白细胞介素-1α 肿瘤坏死因子-α |
| DOI：10.3969/j.issn.1674－070X.2024.11.008 |
| 投稿时间：2024-05-06 |
| 基金项目:湖南省教育厅科学研究项目（21C0249）;湖南中医药大学校级科研基金项目（Z2023XJYB24）;湖南中医药大学本科生科研创新基金项目（2024BKS005）。 |
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| Effects of Runmu Decoction steaming on tear secretion, corneal and lacrimal gland morphology and expressions of inflammation-related proteins in rabbits with aqueous-deficient dry eye |
| XIE Sijian, MA Xiaoya, TANG Yifei, DU Jiaqi, FENG Xian, LAI Juan, WU Jingtao, JIANG Shangping, PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China;The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, China;School of Medicine, Xiamen University, Xiamen, Fujian 361023, China;Ningxiang Hospital of Chinese Medicine, Changsha, Hunan 410000, China) |
| Abstract: |
| Objective To observe the effects of Runmu Decoction steaming on tear secretion, corneal and lacrimal gland morphology, and expression levels of inflammation-related proteins in rabbits with aqueous-deficient dry eye. Methods Thirty healthy New Zealand white rabbits were randomized into blank control group, distilled water group, positive control group, combined treatment group, and Chinese medicine steaming group, with six rabbits in each group. The blank control group received no treatment, while the remaining groups were treated with 1% atropine sulfate ophthalmic gel in the eyes for 3 consecutive days. After successful modeling (starting from the 4th day of the experiment), the blank control group was fed routinely in a normal environment. The distilled water group was given distilled water that had been heated by a Chinese medicine steaming apparatus for steaming the eyes as a control treatment. The positive control group was treated with sodium hyaluronate eye drops, one drop each time, four times a day. The combined treatment group was treated with both steaming with Runmu Decoction and sodium hyaluronate eye drops. The Chinese medicine steaming group was treated with Runmu Decoction that had been heated for steaming the eyes using the Chinese medicine steaming apparatus for 15 minutes each time, once a day. Meanwhile, 1% atropine sulfate eye drops were administered to all groups to maintain the model until the end of the experiment. Schirmer I test (SIT) and corneal fluorescein sodium staining (CFS) scoring were conducted on the 0th, 3rd, 7th, 10th, and 13th days of the experiment. Corneal confocal microscopy was performed on the 3rd and 13th days. The animals were sacrificed on the 13th day. The morphological changes of the corneal epithelium were observed using a light microscope, and the histomorphological changes of the corneal epithelium and lacrimal glands were observed using an electron microscope. The expression levels of interleukin-1α (IL-1α) and tumor necrosis factor-α (TNF-α) in the corneal tissue were measured by Western blot. Results Compared with the blank control group, on the 7th and 10th days, the SIT values of the distilled water and combined treatment groups decreased (P<0.01), and the CFS scores of all groups increased (P<0.05). On the 13th day, the SIT value of the distilled water group decreased (P<0.01), and the CFS scores of the distilled water and positive control groups increased (P<0.05). Compared with the distilled water group, on the 7th day, the SIT values of the positive control and Chinese medicine steaming groups increased (P<0.01), and the CFS score of the combined treatment group decreased (P<0.05). On the 10th and 13th days, the SIT values of the positive control and Chinese medicine steaming groups increased (P<0.01), while their CFS scores decreased (P<0.01). Compared with the positive control group, on the 7th day, the SIT value of the combined treatment group decreased (P<0.05); on the 10th day, its CFS score increased (P<0.05). Compared with the combined treatment group, on the 7th and 10th days, the SIT value of the Chinese medicine steaming group increased (P<0.05). Compared with the blank control group, the density of corneal anterior stroma nerves in the positive control, Chinese medicine steaming, and combined treatment groups recovered to some extent, and the cell morphology was also close to that of the blank control group. No obvious corneal epithelial tissue damage was observed, and the basal cells were neatly arranged, with the morphology similar to that of the blank control group. The abundant cytoplasmic secretion of the lacrimal glands and intact, orderly corneas of the combined treatment group were the closest to those of the blank control group. Compared with the blank control group, the expression levels of IL-1α and TNF-α in the distilled water group increased (P<0.01). Compared with the distilled water group, the expression level of IL-1α in the corneas of the positive control, Chinese medicine steaming, and combined treatment groups decreased (P<0.01), and that of TNF-α in the Chinese medicine steaming and combined treatment groups decreased (P<0.01). Compared with the positive control group, the expression level of TNF-α in the Chinese medicine steaming and combined treatment groups decreased (P<0.05). Conclusion Steaming with Runmu Decoction can promote tear secretion, reduce the damage to corneal epithelium and lacrimal gland tissue, lower the expressions of IL-1α and TNF-α, and thereby inhibit the local inflammatory reaction. |
| Key words: Runmu Decoction Chinese medicine steaming aqueous-deficient dry eye inflammatory factors interleukin-1α tumor necrosis factor-α |
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