| 引用本文: |
易洪芬, 陈昕羽, 彭涵, 肖孟霞, 欧阳里知, 刘红华, 刘迈兰.隔药饼灸对动脉粥样硬化兔血清Ox-LDL、IFN-γ表达的影响[J].湖南中医药大学学报,2024,44(9):1614-1619[点击复制] |
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| 隔药饼灸对动脉粥样硬化兔血清Ox-LDL、IFN-γ表达的影响 |
| 易洪芬,陈昕羽,彭涵,肖孟霞,欧阳里知,刘红华,刘迈兰 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208;湖南中医药大学护理学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察隔药饼灸对动脉粥样硬化（atherosclerosis, AS）兔血清氧化低密度脂蛋白(oxidized low-density lipoprotein，Ox-LDL)、干扰素（interferon-γ, IFN-γ）的影响，探讨隔药饼灸抗AS的作用机制。方法 将18只新西兰兔随机分为正常组、模型组、隔药饼灸组，每组6只。正常组给予普通饲料喂养，其余2组给予高脂饲料喂养，其中隔药饼灸组边造模边干预：2组穴位（巨阙、天枢、丰隆；心俞、肝俞、脾俞）交替行隔药饼灸干预，每穴灸4壮，每日1次，干预12周。HE染色观察主动脉组织病理变化，比色法检测总胆固醇（total cholesterol, TC）、甘油三酯（triglyceride, TG）、低密度脂蛋白胆固醇（low density liporotein cholesterol, LDL-C）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol, HDL-C）含量，ELISA法检测Ox-LDL、IFN-γ含量。结果 与正常组比，模型组主动脉内皮明显增厚，平滑肌排列絮乱，泡沫细胞大量聚集，血清TC、TG、LDL-C、Ox-LDL、IFN-γ含量均显著升高（P＜0.05，P＜0.001），HDL-C含量显著下降（P＜0.001）。与模型组比，隔药饼灸组主动脉内皮结构完整，平滑肌排列整齐，少见泡沫细胞，血清TC、TG、LDL-C、ox-LDL、IFN-γ含量均显著下降（P＜0.05，P＜0.01，P＜0.001），HDL-C含量明显升高（P＜0.05）。结论 隔药饼灸可能通过降低AS兔血清Ox-LDL、IFN-γ含量，减少巨噬细胞脂质摄取，抑制泡沫细胞形成，发挥抗AS的作用。 |
| 关键词: 动脉粥样硬化 隔药饼灸 氧化低密度脂蛋白 干扰素-γ 泡沫细胞 脂质 |
| DOI：10.3969/j.issn.1674-070X.2024.09.009 |
| 投稿时间：2024-05-16 |
| 基金项目:国家自然科学基金面上项目（82074559）；湖南省青年科技创新人才项目（2022RC1222）；湖南省中医药管理局一般课题（202206）；长沙市杰出创新青年培养计划项目（kq1905036）；湖南省教育厅优秀青年项目（22B0377）。 |
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| Effects of moxibustion on medicines on serum expressions of Ox-LDL and IFN-γ in rabbits with atherosclerosis |
| YI Hongfen, CHEN Xinyu, PENG Han, XIAO Mengxia, OUYANG Lizhi, LIU Honghua, LIU Mailan |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;School of Nursing, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of moxibustion on medicines on serum levels of oxidized low-density lipoprotein (Ox-LDL) and interferon-γ (IFN-γ) in rabbits with atherosclerosis (AS), and to explore the anti-AS mechanism of moxibustion on medicines. Methods Eighteen New Zealand rabbits were randomly divided into normal group, model group, and moxibustion on medicines group, with six rabbits in each group. The normal group was fed a normal diet, while the other two groups were fed a high-fat diet. The moxibustion on medicines group received intervention during the modeling process. Alternating moxibustion on medicines was applied to two sets of acupoints ["Juque" (CV14), "Tianshu" (S25), "Fenglong" (S40); "Xinshu" (BL15), "Ganshu"(BL18), and "Pishu"(BL20)] , with four cones per acupoint, once daily for 12 weeks. The pathological changes of aorta were observed using HE staining. Total cholesterol (TC), triglyceride (TG), low density liporotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C) content were measured by colorimetric method. Ox-LDL and IFN-γ levels were determined by ELISA. Results Compared with the normal group, the model group showed significant thickening of the aortic endothelium, disordered arrangement of smooth muscle cells, and massive accumulation of foam cells. Serum content of TC, TG, LDL-C, Ox-LDL, and IFN-γ were significantly elevated (P＜0.05, P＜0.001), while HDL-C content was significantly lower (P＜0.001). Compared with the model group, the moxibustion on medicines group showed intact aortic endothelial structure, orderly arrangement of smooth muscle cells, and rare foam cells. Serum content of TC, TG, LDL-C, ox-LDL, and IFN-γ were significantly lower (P＜0.05, P＜0.01, P＜0.001), while HDL-C content was significantly higher (P＜0.05). Conclusion Moxibustion on medicines may exert anti-AS effects by decreasing serum content of Ox-LDL and IFN-γ in rabbits with AS, thereby reducing macrophage lipid uptake and inhibiting the formation of foam cells. |
| Key words: atherosclerosis moxibustion on medicines oxidized low density lipoprotein interferon-γ foam cells lipids |
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