| 引用本文: |
陈飞,魏谭军,肖成,魏旭,徐飞,王春龙,李先海,王毅.基于多指标成分定量、化学计量学、EW-TOPSIS分析的白术厚朴合剂质量评价研究[J].湖南中医药大学学报,2024,45(3):401-407[点击复制] |
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| 基于多指标成分定量、化学计量学、EW-TOPSIS分析的白术厚朴合剂质量评价研究 |
| 陈飞,魏谭军,肖成,魏旭,徐飞,王春龙,李先海,王毅 |
| (达州市中西医结合医院(达州市第二人民医院)药学科, 四川 达州 635000) |
| 摘要: |
| 目的 以10种成分为指标，建立白术厚朴合剂HPLC定量分析方法，联合化学计量学及熵权优劣解距离法（EW-TOPSIS）对其质量进行综合评价。方法 以75%甲醇提取液为样品，采用Agilent TC-C18色谱柱，乙腈-0.1%磷酸为流动相梯度洗脱，HPLC法同时测定12批白术厚朴合剂中10个化合物（白术内酯Ⅲ、白术内酯Ⅰ、肉桂醇、肉桂酸、桂皮醛、和厚朴酚、厚朴酚、6-姜辣素、8-姜酚和10-姜酚）含量，运用化学计量学和EW-TOPSIS法对含量数据进行分析。结果 经方法学验证，各指标均符合《中华人民共和国药典》要求：10种成分在各自质量浓度范围内线性关系良好，相关系数>0.999；精密度、稳定性、重复性、加样回收率RSD均<2.0%。化学计量学分析显示，前2个主成分代表10个成分92.24%的信息，12批样品聚为3类，桂皮醛、厚朴酚、和厚朴酚、白术内酯Ⅲ、6-姜辣素和白术内酯Ⅰ对白术厚朴合剂的质量影响较大。EW-TOPSIS法结果显示，12批白术厚朴合剂的最优解欧氏贴近度均值为0.238 5～0.666 2，各批次间质量差异较大。结论 建立的多指标成分定量方法操作便捷、结果准确，可用于白术厚朴合剂的质量控制，化学计量学及EW-TOPSIS分析可实现对白术厚朴合剂质量的综合评价，为白术厚朴合剂质量标准提升提供参考。 |
| 关键词: 白术厚朴合剂 高效液相色谱法 化学计量学 多指标成分 熵权优劣解距离法 质量评价 |
| DOI：10.3969/j.issn.1674-070X.2024.03.008 |
| 投稿时间：2023-06-02 |
| 基金项目:四川省中医药管理局中医药科研专项课题（2023MS261）；四川省医学会科研课题计划项目（S22072）。 |
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| Quality evaluation of Baizhu Houpo Mixture based on multi-index component quantification, chemometrics, and EW-TOPSIS analysis |
| CHEN Fei,WEI Tanjun,XIAO Cheng,WEI Xu,XU Fei,WANG Chunlong,LI Xianhai,WANG Yi |
| (Department of Pharmacy, Dazhou Integrated TCM & Western Medicine Hospital (Dazhou Second People's Hospital), Dazhou, Sichuan 635000, China) |
| Abstract: |
| Objective Ten components were selected as indicators to establish a high performance liquid chromatography (HPLC) quantitative analysis method for Baizhu Houpo Mixture, and to comprehensively evaluate its quality through chemometrics and entropy weight solution distance method (EW-TOPSIS). Methods The 75% methanol extract was used as the sample, and the Agilent TC-C18 chromatographic column was used with acetonitrile-0.1% phosphoric acid as the mobile phase for gradient elution. HPLC was used to simultaneously determine the content of ten compounds (atractylenolide Ⅲ, atracylenolide Ⅰ, cinnamyl alcohol, cinnamic acid, cinnamaldehyde, honokiol, magnolol, 6-gingerol, 8-gingerol, and 10-gingerol) in 12 batches of Baizhu Houpo Mixture, and the content data were analyzed by chemometrics and EW-TOPSIS method. Results The methodological verification showed that all the indexes met the requirements of Chinese Pharmacopoeia (ten components had good linear relationships in their respective mass concentration ranges, with correlation coefficients>0.999; the RSDs of precision, stability, repeatability and recovery rate were all<2.0%). Chemometrics analysis revealed that the first two principal components represented 92.24% of the information of the ten components, and the 12 batches of samples were clustered into three categories. Cinnamaldehyde, magnolol, honokiol, atractylenolide III, 6-gingerol and atractylenolide I had a great influence on the quality of Baizhu Houpo Mixture. The results of the EW-TOPSIS method showed that the average Euclidean proximity of the optimal solution among the 12 batches of Baizhu Houpo Mixture ranged from 0.238 5-0.666 2, and the quality of each batch was quite different. Conclusion The established multi-index component quantification method is convenient to operate and accurate, and can be used for quality control of Baizhu Houpo Mixture. Moreover, chemometrics and EW-TOPSIS analysis can realize the comprehensive evaluation of the quality of Baizhu Houpo Mixture, providing a reference for the improvement of its quality standards. |
| Key words: Baizhu Houpo Mixture high performance liquid chromatography chemometrics multi-index components entropy weight solution distance method quality evaluation |
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