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刘芳榕,徐翠萍,刘朝圣,成雪,程晓,张语林.止痒润肤乳对特应性皮炎小鼠模型皮损组织IL-4、IFN-γ蛋白质与基因表达的影响[J].湖南中医药大学学报,2024,44(1):22-29[点击复制] |
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| 止痒润肤乳对特应性皮炎小鼠模型皮损组织IL-4、IFN-γ蛋白质与基因表达的影响 |
| 刘芳榕,徐翠萍,刘朝圣,成雪,程晓,张语林 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;浏阳市中医医院, 湖南 浏阳 410399) |
| 摘要: |
| 目的 观察止痒润肤乳对小鼠特应性皮炎（atopic dermatitis， AD）样模型皮损组织中Janus激酶信号转导子和转录激活子（janus kinase-signal transducer and activator of transcription， JAK-STAT）信号转导通路关键细胞因子白细胞介素-4（interleukin-4， IL-4）、γ干扰素（interferon-γ， IFN-γ）表达水平的影响。方法 将50只SPF级BALB/c小鼠随机分为空白组、模型组、糠酸莫米松软膏组、迪高替尼软膏组、止痒润肤乳组，每组10只。采用2，4-二硝基氯苯（2，4-dinitrochlorobenzene， DNCB）溶液诱导AD样反应，实验过程观察小鼠抓挠行为并进行皮损评分，取材后进行HE染色观察皮损组织病理学改变，采用Real-time PCR法和Western blot法分别测定皮损组织中IL-4、IFN-γ的mRNA表达水平和蛋白含量。结果 与空白组相比，模型组小鼠皮损组织IL-4mRNA和蛋白表达量上升（P<0.05），IFN-γ mRNA和蛋白表达量下降（P<0.05）；与模型组相比，止痒润肤乳组、糠酸莫米松软膏组、迪高替尼软膏组IL-4 mRNA和蛋白表达量减少（P<0.01），IFN-γ mRNA和蛋白表达量升高（P<0.01）；与糠酸莫米松软膏组相比，止痒润肤乳组IL-4 mRNA和蛋白表达量降低（P<0.01）、IFN-γ mRNA和蛋白表达明显升高（P<0.01）；与迪高替尼软膏组相比，止痒润肤乳组IL-4 mRNA 和蛋白表达量差异无统计学意义（P>0.05），IFN-γ mRNA和蛋白表达量升高（P<0.01）。结论 止痒润肤乳对DNCB诱导的AD样症状有明显疗效，其作用机制可能通过抑制JAK-STAT信号转导通路中关键细胞因子IL-4基因表达、上调IFN-γ基因表达，进而减轻炎症反应来实现的。 |
| 关键词: 特应性皮炎 止痒润肤乳 JAK-STAT信号通路 白细胞介素-4 γ干扰素 迪高替尼软膏 |
| DOI：10.3969/j.issn.1674-070X.2024.01.004 |
| 投稿时间：2023-08-20 |
| 基金项目:湖南省临床医疗技术创新引导项目（2021SK51503）；湖南中医药大学研究生创新课题（2022CX149）。 |
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| Effects of Zhiyang Runfu Cream on protein and gene expressions of IL-4 and IFN-γ in skin lesions of mice with atopic dermatitis |
| LIU Fangrong,XU Cuiping,LIU Chaosheng,CHENG Xue,CHENG Xiao,ZHANG Yulin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Liuyang Hospital of Chinese Medicine, Liuyang, Hunan 410399, China) |
| Abstract: |
| Objective To observe the effects of Zhiyang Runfu Cream on the expression levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ), the key cytokines of janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway in skin lesions of mice with atopic dermatitis (AD). Methods Fifty SPF BALB/c mice were randomized into blank, model, Mometasone Furoate Ointment (MFO), Delgocitinib Ointment (DO), and Zhiyang Runfu Cream (ZYRFC) groups, with 10 rats in each group. The AD-like reaction was induced by 2,4-dinitrochlorobenzene (DNCB) solution. The scratching behavior of mice was observed and the condition of skin lesions was scored during the experiment. The AD lesions of mice were peeled back for pathological observation through HE staining. The mRNA expression levels and protein content of IL-4 and IFN-γ in the AD lesions were determined by Real-time PCR and Western blot, respectively. Results Compared with blank group, the mRNA and protein expression levels of IL-4 in the AD lesions of model group increased (P<0.05), while those of IFN-γ decreased (P<0.05). Compared with model group, the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC, MFO, and DO groups were reduced (P<0.01), while those of IFN-γ increased (P<0.01). Compared with MFO group, the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were lower (P<0.01), while those of IFN-γ were significantly higher (P<0.01). Compared with DO group, the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were not significantly different (P>0.05), while those of IFN-γ were elevated (P<0.01). Conclusion Zhiyang Runfu Cream has obvious curative effects on AD-like symptoms induced by DNCB. And its mechanism of action is probably inhibiting the expression of IL-4 gene and increasing the expression of IFN-γ gene in the JAK-STAT signal transduction pathway, and thus alleviating inflammatory reaction. |
| Key words: atopic dermatitis Zhiyang Runfu Cream JAK-STAT signaling pathway interleukin-4 interferon-γ Digotinib Ointment |
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