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朱沁泉,张涤.降气平喘汤对哮喘大鼠气道重塑相关炎症因子及ADAM33、ETS-1表达的影响[J].湖南中医药大学学报,2023,43(11):1986-1991[点击复制] |
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| 降气平喘汤对哮喘大鼠气道重塑相关炎症因子及ADAM33、ETS-1表达的影响 |
| 朱沁泉,张涤 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨降气平喘汤对哮喘大鼠气道重塑,解整合素金属蛋白酶33(a disintegrin and metalloprotease 33, ADAM33)、E26转录因子-1(E26 transformation-specific, ETS-1)表达的影响及干预机制。方法 60只雄性SD大鼠中随机选取15只为正常组。余下大鼠造模,造模成功大鼠随机分为模型组、西药组、中药组,每组15只。除正常组外,其余组大鼠用卵蛋白(ovalbumin, OVA)致敏和激发构建哮喘大鼠模型。实验第54天灌胃干预各组大鼠,中药组予降气平喘汤(生药量8.06 g/kg),西药组予孟鲁司特钠(2.6 mg/kg),正常组和模型组予生理盐水,给药剂量均为10 mL/kg。HE染色观察肺组织病理变化,ELISA检测肺组织白细胞介素-1β(interleukin 1 beta, IL-1β)、白细胞介素-6(interleukin 6, IL-6)、转化生长因子β1(transforming growth factor β1, TGF-β1)水平,Western blot检测肺组织ADAM33、ETS-1蛋白表达水平,real-time PCR检测肺组织ADAM33 mRNA的表达水平。结果 与正常组比较,模型组出现哮喘典型的气道重塑病理变化,肺组织中IL-1β、IL-6、TGF-β1、ADAM33蛋白、ETS-1蛋白、ADAM33 mRNA表达水平明显升高(P＜0.05);与模型组比较,中药组、西药组肺组织病理变化明显改善,肺组织中IL-1β、IL-6、TGF-β1、ADAM33蛋白、ETS-1蛋白、ADAM33 mRNA表达水平明显降低(P＜0.05)。结论 降气平喘汤可通过降低IL-1β、IL-6、TGF-β1炎症因子分泌、降低ADAM33、ETS-1的表达,改善哮喘大鼠的气道重塑,实现对哮喘的干预作用。 |
| 关键词: 降气平喘汤 哮喘 气道重塑 炎症因子 ADAM33 ETS-1 |
| DOI：10.3969/j.issn.1674-070X.2023.11.008 |
| 投稿时间：2023-09-04 |
| 基金项目:湖南省教育厅科研项目（18C0397）；湖南省政府特批专项张涤中医儿科临床研究所建设项目（99-16）；湖南中医药大学中医学一流学科开放基金项目（2022ZYX02）。 |
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| Effects of Jiangqi Pingchuan Decoction on airway remodeling related inflammatory factors and expressions of ADAM33 and ETS-1 in asthmatic rats |
| ZHU Qinquan,ZHANG Di |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effects of Jiangqi Pingchuan Decoction on airway remodeling related inflammatory factors and expressions of a disintegrin and metalloprotease 33 (ADAM33) and E26 transformation-specific (ETS-1) in asthmatic rats and its intervention mechanism. Methods Fifteen rats were randomly selected as the normal group in 60 male SD rats. The remaining rats were randomly assigned into model, western medicine, and Chinese medicine groups, with 15 rats in each group. Except the normal group, the rats in other groups were sensitized and stimulated with ovalbumin (OVA) to construct the asthma rat model. On the 54th day of the experiment, the Chinese medicine group was given Jiangqi Pingchuan Decoction (crude drug amount:8.06 g/kg), the western medicine group was given montelukast sodium (2.6 mg/kg), and the normal group and model group were given normal saline (10 mL/kg). HE staining was used to observe the pathological changes of lung tissue, and ELISA was taken to determine interleukin 1 beta (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1) in lung tissue. The expression levels of ADAM33 and ETS-1 protein in lung tissue were checked by Western blot, and the expression levels of ADAM33 mRNA in lung tissue were examine by real-time PCR. Results Compared with normal group, the model group showed typical pathological changes of airway remodeling, and expression levels of IL-1β, IL-6, TGF-β1, ADAM33 protein, ETS-1 protein, and ADAM33 mRNA increased significantly (P＜0.05); compared with model group, the pathological changes of lung tissue in Chinese medicine and Western medicine groups were notably improved, and expression levels of IL-1β, IL-6, TGF-β1, ADAM33 protein, ETS-1 protein, and ADAM33 mRNA decreased significantly (P＜0.05). Conclusion Jiangqi Pingchuan Decoction can improve airway remodeling in asthmatic rats and intervene asthma by decreasing the expression levels of inflammatory factors such as IL-1β, IL-6, and TGF-β1, and the expression of ADAM33 and ETS-1. |
| Key words: Jiangqi Pingchuan Decoction asthma airway remodeling inflammatory factors ADAM33 ETS-1 |
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