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吴淑辉,刘娟,肖婷,郑慧娥,朱明芳.丹润方HPLC指纹图谱及多成分含量测定研究[J].湖南中医药大学学报,2023,43(10):1822-1829[点击复制] |
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| 丹润方HPLC指纹图谱及多成分含量测定研究 |
| 吴淑辉,刘娟,肖婷,郑慧娥,朱明芳 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学第二附属医院, 湖南 长沙 410005;血管生物学与转化医学湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 建立丹润方指纹图谱并测定没食子酸、绿原酸、鞣花酸含量，为其质量控制提供依据。方法 采用Agilent 5 TC-C18色谱柱，以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，流速1.0 mL·min-1，检测波长315 nm，柱温30 ℃；通过相似度评价，结合主成分分析及偏最小二乘法-判别分析对15批丹润方指纹图谱进行分析评价，并对3种指标成分进行定量测定。结果 建立了15批丹润方指纹图谱，共标定19个共有峰，各批次的相似度均大于0.9；没食子酸、绿原酸、鞣花酸在各自线性范围内线性关系良好；15批丹润方中没食子酸、绿原酸、鞣花酸的质量分数为0.219~1.100、0.168~0.445、0.418~3.077 mg/g。结论 建立的丹润方指纹图谱及多成分定量测定方法简单、准确、重现性较好，可用于丹润方整体质量评价。 |
| 关键词: 丹润方 指纹图谱 质量评价 没食子酸 绿原酸 鞣花酸 |
| DOI：10.3969/j.issn.1674-070X.2023.10.011 |
| 投稿时间：2023-03-17 |
| 基金项目:湖南省自然科学基金科药联合基金项目（2020JJ9051）；湖南省卫生健康委员会科研项目（202202124772）。 |
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| HPLC fingerprint and multi-component content determination of Danrun Formula |
| WU Shuhui,LIU Juan,XIAO Ting,ZHENG Hui'e,ZHU Mingfang |
| (The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Key Laboratory of Vascular Biology and Translational Medicine, Education Department of Hunan Province, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To establish the HPLC fingerprint of Danrun Formula and determine the content of gallic acid, chlorogenic acid, and ellagic acid so as to provide basis for its quality control. Methods Agilent 5 TC-C18 chromatographic column was used, with the mobile phase of cetonitrile-0.1% phosphoric acid aqueous solution for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was 315 nm, and the column temperature was 30 ℃. Through similarity evaluation, combined with principal component analysis and partial least squares-discriminant analysis, the fingerprints of 15 batches of Danrun Formula were analyzed and evaluated, and the 3 index components were quantitatively determined. Results The fingerprints of 15 batches of Danrun Formula were established with 19 common peaks, and the similarity of each batch was greater than 0.9. Gallic acid, chlorogenic acid, and ellagic acid had good linear relationships within their respective linear ranges. The mass fractions of gallic acid, chlorogenic acid, and ellagic acid in 15 batches of Danrun Formula were 0.219-1.100, 0.168-0.445, and 0.418-3.077 mg/g, respectively. Conclusion The established fingerprint and multi-component quantitative determination method of Danrun Formula are simple, accurate, and reproducible, which can be used for the overall quality evaluation of Danrun Formula. |
| Key words: Danrun Formula fingerprint quality evaluation gallic acid chlorogenic acid ellagic acid |
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