| 引用本文: |
聂格,李晓玲,石雨荷,李晴,朱珏,侯超文,刘湘丹,童巧珍.健脾扶正颗粒HPLC指纹图谱的建立及8个化学成分含量测定[J].湖南中医药大学学报,2023,43(9):1609-1616[点击复制] |
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| 健脾扶正颗粒HPLC指纹图谱的建立及8个化学成分含量测定 |
| 聂格,李晓玲,石雨荷,李晴,朱珏,侯超文,刘湘丹,童巧珍 |
| (湖南中医药大学, 湖南 长沙 410208;浏阳市中医医院, 湖南 浏阳 410300;湖南中医药大学附属第二中西医结合医院, 湖南 浏阳 410300) |
| 摘要: |
| 目的 建立15批健脾扶正颗粒HPLC指纹图谱，并测定其中8个有效成分黄芪甲苷、毛蕊异黄酮糖苷、芒柄花素、桔梗皂苷D、五味子醇甲、茯苓酸、太子参环肽B、葫芦巴碱的含量。方法 采用Waters Sun fire C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.1%磷酸水溶液为流动相，流速1.0 mL·min-1，检测波长203、210、250 nm切换，梯度洗脱，建立健脾扶正颗粒指纹图谱及8个化学成分的含量测定。结果 建立的健脾扶正颗粒HPLC指纹图谱共标定33个共有峰，通过标准品比对，指认了8个色谱峰，其在一定浓度范围内线性良好，平均加样回收率良好，15批样品的指纹图谱相似度均在0.90以上，最后通过聚类分析和主成分分析把15批样品分为2类，以S1样品的综合得分最高、整体质量最好。结论 所建立指纹图谱重复性好、专属性强，可为健脾扶正颗粒质量控制标准的制定提供参考。 |
| 关键词: 健脾扶正颗粒 HPLC 指纹图谱 含量测定 黄芪甲苷 |
| DOI：10.3969/j.issn.1674-070X.2023.09.009 |
| 投稿时间：2023-02-16 |
| 基金项目:湖南省自然科学基金项目（2020JJ4466）；湖南省卫生健康委员会重点项目（202113051021）。 |
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| Establishment of HPLC fingerprints and content determination of eight chemical components of Jianpi Fuzheng Granules |
| NIE Ge,LI Xiaoling,SHI Yuhe,LI Qing,ZHU Jue,HOU Chaowen,LIU Xiangdan,TONG Qiaozhen |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Liuyang Hospital of Chinese Medicine, Liuyang, Hunan 410300, China;The Second Hospital of Integrative Medicine of Hunan University of Chinese Medicine, Liuyang, Hunan 410300, China) |
| Abstract: |
| Objective To establish high-performance liquid chromatographic fingerprints of 15 batches of Jianpi Fuzheng Granules, and to determine the content of eight effective components, namely, astragaloside Iv, mullein isoflavone glycoside, mangiferin, platycodin D, schisandrin, pachymic acid, heterophyllin B, and trigonelline. Methods A Waters Sun fire C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase, with a flow rate of 1.0 ml·min-1 and the detection wavelengths of 203, 210, and 250 nm switching. Gradient elution was performed to establish the fingerprints of Jianpi Fuzheng Granules and determine the content of eight chemical components. Results The established HPLC fingerprint of Jianpi Fuzheng Granules calibrated a total of 33 common peaks. Through standard comparison, eight chromatographic peaks were identified, which showed good linearity with a certain concentration range and good average sample recovery. The similarity of the fingerprint of 15 batches of samples was above 0.90. Finally, the 15 batches of samples were divided into 2 categories by cluster analysis and principal component analysis, with S1 sample having the highest comprehensive score and the best overall quality. Conclusion The fingerprints established are reproducible and specific, which can provide reference for the development of quality control standards for Jianpi Fuzheng Granules. |
| Key words: Jianpi Fuzheng Granules HPLC fingerprint content determination astragaloside |
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