| 引用本文: |
张曦宁,董振华,赵震宇,彭娜姿,胡志希,李琳.真武汤对阿霉素诱导的慢性心力衰竭大鼠肠黏膜细菌多样性的影响[J].湖南中医药大学学报,2023,43(8):1368-1378[点击复制] |
|
| |
|
|
| 本文已被：浏览 1859次 下载 627次 |
| 真武汤对阿霉素诱导的慢性心力衰竭大鼠肠黏膜细菌多样性的影响 |
| 张曦宁,董振华,赵震宇,彭娜姿,胡志希,李琳 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学中医诊断研究所, 湖南 长沙 410208) |
| 摘要: |
| 目的 检测真武汤对慢性心力衰竭大鼠肠道菌群的影响，探讨真武汤治疗慢性心力衰竭的作用机制。方法 24只大鼠分为空白组、模型组、真武汤组、美托洛尔组，每组6只，模型组、真武汤组及美托洛尔组采用腹腔注射阿霉素（3 mg/kg，每周1次，连续7周）的方法制备心力衰竭模型，造模成功后，真武汤组进行真武汤18 g/（kg·d）灌胃治疗，美托洛尔组用美托洛尔溶液10 mg/（kg·d）灌胃治疗，空白组和模型组进行蒸馏水10 mL/（kg·d）灌胃，每天1次，连续21 d。干预结束后采集黏膜刮片，进行Miseq高通量测序。结果 治疗后，模型组左室射血分数（left ventricular ejection fraction，LVEF）和左心室短轴缩短率（left ventricular ejection fractional shortening，LVFS）均明显低于空白组（P<0.01），真武汤组LVEF、LVFS均明显高于模型组（P<0.01）。与空白组相比，模型组大鼠肠道菌群Chao、Shannon、ACE指数显著升高（P<0.05，P<0.01），Simpson指数显著下降（P<0.05）；与模型组相比，真武汤组各项指标出现回调。与空白组相比，模型组变形菌显著减少（P<0.01），脱硫杆菌显著增加（P<0.01），拟杆菌门、疣微菌门、酸杆菌门、螺旋菌门增加（P<0.05）；与模型组相比，真武汤组变形菌、脱硫杆菌、疣微菌、酸杆菌及蛭弧菌门出现回调（P<0.05）。与空白组比较，模型组大肠志贺菌属、瘤胃球菌科显著下降（P<0.05，P<0.01），未分类的绒毛杆菌属、罗姆布茨菌属、杜氏杆菌、狭义梭菌属、颤螺旋菌科、双歧杆菌属、普雷沃菌属显著增加（P<0.05，P<0.01）；与模型组比较，真武汤组大肠志贺菌属、乳酸杆菌属、杜氏杆菌呈显著回调趋势（P<0.05，P<0.01）。结论 真武汤可有效改善慢性心力衰竭大鼠肠道菌群多样性及丰富度，调节肠黏膜细菌组成与结构，这或为真武汤治疗慢性心力衰竭的潜在作用机制。 |
| 关键词: 心力衰竭 真武汤 肠黏膜细菌 中医药 16S rRNA 高通量测序 生物信息学 |
| DOI：10.3969/j.issn.1674-070X.2023.08.005 |
| 投稿时间：2022-11-21 |
| 基金项目:国家自然科学基金项目（82274412）；湖南省自然科学基金项目（2023JJ30453）；湖南省中医药科研项目（B2023045）；广东省重点领域研发项目（2020B1111100001）；长沙市自然科学基金项目（kq2208185）。 |
|
| Effects of Zhenwu Decoction on bacterial diversity of intestinal mucosa in rats with doxorubicin-induced chronic heart failure |
| ZHANG Xining,DONG Zhenhua,ZHAO Zhenyu,PENG Nazi,HU Zhixi,LI Lin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of Zhenwu Decoction on the intestinal flora of rats with chronic heart failure (HF), and to explore the mechanism of action of Zhenwu Decoction in treating this disease. Methods Twenty-four rats were divided into blank group, model group, Zhenwu Decoction group, and metoprolol group, with six rats in each group, and HF models in model group, Zhenwu Decoction group, and metoprolol group were established by intraperitoneal injection of doxorubicin (3 mg/kg, once a week, for 7 consecutive weeks). After successful modeling, Zhenwu Decoction group was given Zhenwu Decoction 18 g/(kg·d) by gavage, metoprolol group metoprolol solution 10 mg/(kg·d), and blank group and model group distilled water 10 mL/(kg·d), once a day, for 21 consecutive days. After the intervention, the intestinal mucosal scrapings of the rats were collected for high-throughput sequencing of Miseq. Results After treatment, the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) of rats in model group were significantly lower than those in blank group (P<0.01); compared with model group, LVEF and LVFS of rats in Zhenwu Decoction group were significantly higher (P<0.01). Compared with blank group, the Chao, Shannon and ACE indexes of rat intestinal flora in model group were significantly higher (P<0.05, P<0.01), while Simpson index was significantly lower (P<0.05); compared with model group, those indexes in Zhenwu Decoction group showed a callback. Compared with blank group, the intestinal flora of rats in model group showed a significant decrease in the abundance of Proteobacteria (P<0.01), a significant increase in the abundance of Desulfobacterota (P<0.01), and an increase in the abundance of Bacteroidetes, Verrucomicr-obia, Acidobacteria, and Spirochaetes (P<0.05); compared with model group, the abundance of Proteobacteria, Desulfobacterota, Verrucomicrobia, Acidobacteria, and Bdellovibrionota in Zhenwu Decoction group showed a callback (P<0.05). Compared with blank group, the intestinal flora of rats in model group showed a significant decrease in the abundance of Escherichia-Shigella and norank_f__Ruminococcaceae (P<0.05, P<0.01), and a significant increase in the abundance of norank_f__Muribaculaceae, Romboutsia, Dubosiella, Clostridium_sensu_stricto_1, norank_f__Oscillospiraceae, Bifidobacterium, and Prevotella (P<0.05, P<0.01); compared with model group, the abundance of Escherichia-Shigella, Lactobacillus, and Dubosiella showed a significant callback in Zhenwu Decoction group (P<0.05, P<0.01). Conclusion Zhenwu Decoction can effectively improve the diversity and abundance of intestinal flora in rats with chronic HF, and regulate the composition and structure of intestinal mucosal bacteria, which may be the potential mechanism of Zhenwu Decoction in treating chronic HF. |
| Key words: heart failure Zhenwu Decoction intestinal mucosal bacteria Chinese medicine 16S rRNA high-throughput sequencing bioinformatics |
|
二维码（扫一下试试看！） |
|
|
|
|
