| 引用本文: |
周赛男,蔺晓源,陈思清,周姝,韩运宗,刘琴,符佳.四磨汤对功能性消化不良大鼠CNP/NPR-B/cGMP信号通路的影响[J].湖南中医药大学学报,2023,43(7):1165-1172[点击复制] |
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| 四磨汤对功能性消化不良大鼠CNP/NPR-B/cGMP信号通路的影响 |
| 周赛男,蔺晓源,陈思清,周姝,韩运宗,刘琴,符佳 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过研究四磨汤对功能性消化不良（functional dyspepsia，FD）模型大鼠CNP/NPR-B/cGMP信号通路的影响，进一步探讨其作用机制。方法 将Wistar大鼠随机分为对照组、模型组、低浓度四磨汤组、中浓度四磨汤组、高浓度四磨汤组以及莫沙必利组。参考文献中的方法使用夹尾刺激联合不规则饮食14 d制备FD大鼠模型。造模结束后，四磨汤低、中、高浓度组大鼠分别灌胃1.8、2.4、3.6 g/（kg·d）剂量的四磨汤药液，莫沙必利组灌胃0.305 mg/（kg·d）剂量的莫沙必利药液，对照组和模型组给予等体积蒸馏水，给药14 d。观测大鼠体质量变化；酚红法检测大鼠胃排空率；碳粉法检测大鼠小肠推进率；ELISA法检测血清中胃动素（motilin，MTL）、胃泌素（gastrin，GAS）、胃窦平滑肌C型利钠肽（C-type natriuretic peptide，CNP）、环磷酸鸟苷（cyclic guanosine monophosphate，cGMP）的含量；RT-qPCR和Western blot分别检测胃窦平滑肌CNP、B型尿钠肽受体（type B urinary natriuretic peptide receptor，NPR-B）的基因和蛋白表达。结果 与对照组比较，模型组大鼠体质量降低（P<0.05），胃排空率与小肠推进率明显减弱（P<0.05），血清MTL、GAS含量降低（P<0.05），胃窦平滑肌CNP、cGMP含量升高（P<0.05），CNP、NPR-B的mRNA和蛋白表达增加（P<0.05）。与模型组相比，四磨汤各剂量组大鼠体质量显著增加（P<0.05），胃排空率和小肠推进率显著提高（P<0.05），血清MTL和GAS含量显著增加（P<0.05），胃窦平滑肌CNP、cGMP含量降低（P<0.05），CNP、NPR-B的mRNA和蛋白表达降低（P<0.05）。结论 四磨汤通过抑制CNP/NPR-B/cGMP信号通路以促进胃肠动力可能是其治疗FD的作用机制之一。 |
| 关键词: 功能性消化不良 四磨汤 CNP/NPR-B/cGMP信号通路 大鼠 胃排空率 小肠推进率 |
| DOI：10.3969/j.issn.1674-070X.2023.07.003 |
| 投稿时间：2023-01-13 |
| 基金项目:湖南省中医药科研计划重点项目（2021203）；湖南省自然科学基金科卫联合项目（2022JJ70031）；湖南中医药大学中医学一流学科开放基金项目（2018ZYX13）；湖南中医药大学中医学国内一流学科项目（4901-020000200205）。 |
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| Effects of Simo Decoction on CNP/NPR-B/cGMP signaling pathway in rats with functional dyspepsia |
| ZHOU Sainan,LIN Xiaoyuan,CHEN Siqing,ZHOU Shu,HAN Yunzong,LIU Qin,FU Jia |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of Simo Decoction on CNP/NPR-B/cGMP signaling pathway in rats of functional dyspepsia (FD) model, so as to further explore its mechanism of action. Methods Wistar rats were randomly divided into control group, model group, mosapride group, low-, medium-, and high-dose Simo Decoction groups. The FD rat model was established by tail-clipping stimulation combined with irregular diet for 14 d. After modeling, the rats in low-, medium-, and high-dose Simo Decoction groups were intragastrically administered with 1.8, 2.4, and 3.6 g/(kg·d) Simo Decoction, respectively. Mosapride group was received 0.305 mg/(kg·d) mosapride solution by gavage, while control and model groups were given an equal volume of distilled water, and all the administration lasted for 14 d. The changes in body weight of the rats were observed; the gastric emptying rate was measured by the phenol red method; the small intestinal propulsive rate was measured by the carbon powder method; the levels of motilin (MTL), gastrin (GAS), C-type natriuretic peptid (CNP), and cyclic guanosine monophosphate (cGMP) in serum were determined by ELISA; the gene and protein expressions of CNP and Type B urinary natriuretic peptide receptor (NPR-B) in gastric antrum smooth muscle were examined by RT-qPCR and Western blot, respectively. Results Compared with control group, the rats in model group showed a decrease in body weight (P<0.05), significantly decreased gastric emptying and small intestinal propulsive rates (P<0.05), lower MTL and GAS levels in serum (P<0.05), higher CNP and cGMP levels in gastric antrum smooth muscle (P<0.05), and increased mRNA and protein expressions of CNP and NPR-B (P<0.05). Compared with model group, the body weight, gastric emptying rate, small intestinal propulsive rate, and the levels of MTL and GAS in serum in Simo Decoction groups were significantly higher (P<0.05), while the levels of CNP and cGMP in gastric antrum smooth muscle and mRNA and protein expressions of CNP and NPR-B were lower (P<0.05). Conclusion Simo Decoction may exert its therapeutic effects on FD by inhibiting CNP/NPR-B/cGMP signaling pathway and promoting gastrointestinal motility. |
| Key words: functional dyspepsia Simo Decoction CNP/NPR-B/cGMP signaling pathway rats gastric emptying rate small intestinal propulsive rate |
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