| 引用本文: |
蒋芙蓉,江星明,黄良辉,夏新斌,何雄,袁志鹰.基于ATR-FTIR研究药食同源植物多花黄精的产地分布[J].湖南中医药大学学报,2023,43(5):864-868[点击复制] |
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| 基于ATR-FTIR研究药食同源植物多花黄精的产地分布 |
| 蒋芙蓉,江星明,黄良辉,夏新斌,何雄,袁志鹰 |
| (湖南中医药大学, 湖南 长沙 410208;湖南科伦制药有限公司, 湖南 岳阳 414100;湖南省药品审评与不良反应监测中心, 湖南 长沙 410013;湖南中医药大学, 湖南 长沙 410208;中医心肺病证辨证与药膳食疗重点研究室, 湖南 长沙 410208) |
| 摘要: |
| 目的 基于傅里叶变换衰减全反射红外光谱(attenuated total reflection-flourier transformed infrared spectroscopy, ATR-FTIR)研究药食同源植物多花黄精的产地分布。方法 采用 ATR-FTIR 技术采集不同产地多花黄精粉末样本的红外光谱数据,通过红外谱图的波数分析其对应的官能团,并进行精密度、重复性、稳定性的方法学考察试验。谱图数据经预处理后,建立主成分分析(principal components analysis, PCA)模型和正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis, OPLS-DA)模型,进行多元统计数据分析。结果 不同产地多花黄精ATR-FTIR图谱的峰形、峰位差异微小。运用PCA和OPLS-DA可视化处理不同产地多花黄精的数据,发现7种不同产地多花黄精代谢轮廓差异明显,可对不同产地的多花黄精进行辅助鉴别。结论 ATR-FTIR技术操作简单,环保经济,可为多花黄精质量控制提供技术参考。 |
| 关键词: 多花黄精 傅里叶变换衰减全反射红外光谱 正交偏最小二乘判别分析 产地 质量控制 |
| DOI：10.3969/j.issn.1674-070X.2023.05.015 |
| 投稿时间：2022-09-29 |
| 基金项目:财政部和农业农村部：国家现代农业产业技术体系资助（CARS-21）；湖南省重点研发计划类/高新技术产业科技创新引领计划（2020SK2022，2022SK2018）；建生鲜药创研基金项目（JSYY-20210105-068）；湖南中医药大学化学工程与技术一流学科建设项目资助。 |
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| Geographical distribution of the medicinal and edible plant Polygonatum cyrtonema Hua based on ATR-FTIR |
| JIANG Furong,JIANG Xingming,HUANG Lianghui,XIA Xinbin,HE Xiong,YUAN Zhiying |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Kelun Pharmaceutical Co., Ltd, Yueyang, Hunan 414100, China;Hunan Center for Drug Evaluation&ADR Monitoring, Changsha, Hunan 410013, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory of TCM Heart and Lung Pattern Differentiation, Medicated Diet&Dietotherapy, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the geographical distribution of the medicinal and edible plant Polygonatum cyrtonema Hua based on attenuated total reflection-fourier transform infrared spectroscopy (ATR-FTIR). Methods The infrared spectrum data of Polygonatum cyrtonema Hua powder samples from different places of origin were collected by ATR-FTIR technology. Moreover, the corresponding functional groups were analyzed by the wave number of the infrared spectrum, and the methodological investigation test of the precision, repeatability and stability was carried out. After preprocessing the spectral data, the orthogonal partial least squares-discriminant analysis (OPLS-DA) model and the principal component analysis (PCA) model were established for multivariate statistical data analysis. Results The peak shape and position of the ATR-FTIR spectra of Polygonatum cyrtonema Hua from different places of origin were slightly different. The PCA and OPLS-DA were adopted to visualize the data of Polygonatum cyrtonema Hua from different places of origin. It was found that the metabolic profiles of Polygonatum cyrtonema Hua from 7 different places of origin were significantly different, which could assist in the identification of Polygonatum cyrtonema Hua from different places of origin. Conclusion ATR-FTIR technology is simple to operate, environmental friendly and economic, which can provide technical reference for the quality control of Polygonatum cyrtonema Hua. |
| Key words: Polygonatum cyrtonema Hua attenuated total reflection-flourier transformed infrared spectroscopy orthogonal partial least squares-discriminant analysis places of origin quality control |
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