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欧文静,曹馨月,张天睿,葛东宇,韩冰,刘晓凤,张硕峰.前列闭尔通栓对慢性非细菌性前列腺炎的治疗作用研究[J].湖南中医药大学学报,2022,42(12):1988-1994[点击复制] |
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| 前列闭尔通栓对慢性非细菌性前列腺炎的治疗作用研究 |
| 欧文静,曹馨月,张天睿,葛东宇,韩冰,刘晓凤,张硕峰 |
| (北京中医药大学中药学院, 北京 102488;黑龙江济仁药业有限公司, 黑龙江 哈尔滨 150000) |
| 摘要: |
| 目的 考察前列闭尔通栓对慢性非细菌性前列腺炎(chronic nonbacterial prostatitis, CNP)模型大鼠的治疗作用。方法 将60只大鼠随机均分为空白组、模型组、前列通栓组和前列闭尔通栓低、中、高剂量组,模型组和给药组均注射完全弗氏佐剂0.1 mL 1次。造模1天后直肠给药23 d(前列闭尔通栓低、中、高剂量组分别给药0.33、0.66、0.99 g/d,前列通栓组给药0.42 g/d)。分别从前列腺质量,前列腺指数,HE染色病理情况,膀胱内压,卵磷脂小体,白细胞数量,肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-10(interleukin-10, IL-10)、超氧化物歧化酶(superoxide dismutase, SOD)、白细胞介素-8 (interleukin-8, IL-8)、丙二醛(malondialdehyde, MDA)含量方面进行分析。结果 与空白组相比,模型组前列腺质量增加、前列腺指数升高、病理评分升高(P<0.05),膀胱内压降低、卵磷脂小体数量减少(P<0.05),白细胞数量增多、TNF-α含量升高(P<0.05),IL-10、SOD含量降低(P<0.05),IL-8、MDA含量升高(P<0.05)。与模型组比较,前列闭尔通栓中、高剂量组前列腺质量降低(P<0.05);前列闭尔通栓中剂量组前列腺指数降低(P<0.05)、SOD含量升高(P<0.05);前列闭尔通栓各剂量组病理评分降低(P<0.05)、IL-10含量升高(P<0.05);前列通栓组及前列闭尔通栓各剂量组膀胱内压升高、白细胞数量降低(P<0.05);前列闭尔通栓中、高剂量组及前列通栓组卵磷脂小体数量升高(P<0.05);前列通栓组TNF-α含量降低(P<0.05);前列闭尔通栓高剂量组MDA含量降低(P<0.05)。与前列闭尔通栓低剂量组比较,前列闭尔通栓高剂量组病理评分降低(P<0.05)、膀胱内压增加(P<0.05)、IL-8含量降低(P<0.05);前列闭尔通栓中剂量组SOD含量上升(P<0.05)。与前列闭尔通栓中剂量组相比,前列闭尔通栓低、高剂量组及前列通栓组卵磷脂小体数量降低(P<0.05)、白细胞数量升高(P<0.05)。结论 前列闭尔通栓可能通过降低CNP大鼠的前列腺指数和前列腺质量、升高膀胱内压、减轻病理损伤程度、增加CNP大鼠前列腺液中的卵磷脂小体数量、减少CNP大鼠前列腺液中的白细胞数量,对CNP模型大鼠发挥治疗作用。 |
| 关键词: 前列闭尔通栓 慢性非细菌性前列腺炎 白细胞 肿瘤坏死因子 白细胞介素 丙二醛 超氧化物歧化酶 |
| DOI：10.3969/j.issn.1674-070X.2022.12.006 |
| 投稿时间：2022-05-16 |
| 基金项目:北京中医药大学校级科研纵向发展基金项目(2018-zxfzjj-002) |
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| Therapeutic effects of Qianlie Bi'ertong Suppository on chronic nonbacterial prostatitis |
| OU Wenjing,CAO Xinyue,ZHANG Tianrui,GE Dongyu,HAN Bing,LIU Xiaofeng,ZHANG Shuofeng |
| (School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;Heilongjiang Jiren Pharmaceutical Co., Ltd., Harbin, Heilongjiang 150000, China) |
| Abstract: |
| Objective To investigate the therapeutic effects of Qianlie Bi'ertong Suppository(QLBETS) on model rats with chronic nonbacterial prostatitis(CNP).Methods Sixty rats were randomly divided into blank group, model group, Qianlietong Suppository(QLTS) group and low-dose, medium-dose and high-dose groups of QLBETS. Both model group and medication administration group were injected with 0.1 mL of complete Freund's adjuvant once. One day after modeling, the rats were given rectal administration for 23 d(0.33, 0.66, 0.99 g/d for the low, medium and high dose groups of QLBETS respectively, and 0.42 g/d for the QLTS group). Then prostate weight, prostate index, HE staining pathology, internal pressure of bladder, lecithin body, white blood cell counts, and the blood levels of tumor necrosis factor-α(TNF-α), interleukin-10(IL-10), superoxide dismutase(SOD),interleukin-8(IL-8) and malondialdehyde(MDA) of each group were analyzed.Results Compared with the blank group, the prostate weight, prostate index and pathological score in the model group increased(P<0.05); bladder internal pressure decreased; the number of lecithin bodies decreased(P<0.05); the white blood cell counts increased, the level of TNF-α, IL-8 and MDA increased(P<0.05),and the level of IL-10 and SOD decreased(P<0.05). Compared with the model group, the prostate weight in the medium and high dose groups of QLBETS decreased(P<0.05); prostate index decreased(P<0.05) and SOD level increased(P<0.05) in the medium-dose group of QLBETS; the pathological scores of all QLBETS groups decreased(P<0.05) and the level of IL-10 increased(P<0.05); bladder internal pressure in both QLTS group and QLBETS groups increased and the white blood cell counts decreased(P <0.05); the number of lecithin bodies in the medium and high dose groups of QLBETS and QLTS group increased(P<0.05); TNF-α level in QLTS group decreased(P<0.05); MDA level in the high dose group of QLBETS decreased(P<0.05). Compared with the low-dose group of QLBETS, the pathological score of the high-dose group decreased(P<0.05); the change rate of bladder internal pressure increased(P<0.05), and the level of IL-8 decreased(P<0.05); SOD level in the medium-dose group increased(P<0.05). Compared with the medium-dose group of QLBETS, the number of lecithin bodies in the low and high dose groups of QLBETS and QLTS group decreased(P<0.05), and the leukocyte counts increased(P<0.05).Conclusion QLBETS may exert therapeutic effects on CNP model rats by reducing the prostate index and prostate weight, increasing bladder internal pressure, reducing the degree of pathological damage and the number of leukocyte in the prostatic fluid of CNP rats, as well as increasing the number of lecithin bodies. |
| Key words: Qianlie Bi'ertong Suppository chronic nonbacterial prostatitis white blood cells tumor necrosis factor interleukin malondialdehyde superoxide dismutase |
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