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吴琼,谢宗池,黄河,李甜,朱晓晴,李武,李铁浪.按法干预心俞穴对心肌缺血大鼠心肌保护作用的研究[J].湖南中医药大学学报,2022,42(10):1677-1682[点击复制] |
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| 按法干预心俞穴对心肌缺血大鼠心肌保护作用的研究 |
| 吴琼,谢宗池,黄河,李甜,朱晓晴,李武,李铁浪 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨按法干预心俞穴对心肌缺血大鼠能量代谢的影响，及其对心肌的保护作用机制。方法 36只健康雄性SD大鼠，随机分为3组，假手术组、模型组、按法组，每组12只。假手术组开胸后冠状动脉只扎不结，模型组、按法组采用冠状动脉左前降支结扎术建立大鼠心肌缺血模型。按法组术后第2天介入按法干预，取心俞穴，其余两组不予按法刺激，仅捆绑固定。每次干预或捆绑15 min，每日1次，连续7 d。于造模前后和干预后以小动物心电图机检测大鼠心电图，以酶联免疫吸附测定法检测血清中肌钙蛋白I（cardiac troponin I, cTnI）、肌酸激酶MB同工酶（creatine kinase MB isoenzymes, CK-MB）、乳酸脱氢酶（layered double hydroxide, LDH）含量和心肌组织中三磷酸腺苷（adenosine triphosphate, ATP）、二磷酸腺苷（adenosine diphosphate, ADP）和一磷酸腺苷（adenosine monophosphate, AMP）含量。结果 （1）模型组和按法组大鼠造模后J点振幅均高于造模前（P<0.05）；干预后，与模型组对比，按法组J点振幅降低（P<0.05）。（2）HE染色显示模型组大鼠左心室壁缺血面积大于其余两组，按法组缺血严重程度不如模型组。（3）与假手术组相比，模型组大鼠血清cTnI、CK-MB、LDH含量明显升高（P<0.01），心肌细胞ATP含量、能荷值明显降低（P<0.01），ADP、AMP含量明显升高（P<0.01）；与模型组对比，按法组大鼠血清cTnI、CK-MB、LDH含量明显降低（P<0.01），心肌细胞ATP含量、能荷值明显升高（P<0.01），AMP含量明显降低（P<0.01）。结论 按法干预心俞穴可有效保护缺血心肌，其机制可能是降低心肌细胞ADP、AMP含量，提高ATP含量和能荷值，改善心肌能量代谢和缺血缺氧状态，从而起到对心肌的保护作用。 |
| 关键词: 按法 心肌缺血 大鼠 心俞穴 能量代谢 推拿 三磷酸腺苷 |
| DOI：10.3969/j.issn.1674-070X.2022.10.014 |
| 投稿时间：2022-03-04 |
| 基金项目:国家自然科学基金青年项目（81804216）；湖南中医药大学研究生创新课题（2020CX26）。 |
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| Protective effects of pressing “Xinshu” (B15) on the myocardium in myocardial ischemia rats |
| WU Qiong,XIE Zongchi,HUANG He,LI Tian,ZHU Xiaoqing,LI Wu,LI Tielang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effects of pressing "Xinshu" (B15) on energy metabolism of rats with myocardial ischemia and its protective mechanism on myocardium. Methods Thirty six healthy male SD rats were randomly divided into sham-operated group, model group, and pressing group, with 12 rats in each. For the sham-operated group, the coronary artery was threaded but not ligatured; for the model group and pressing group, the models of myocardial ischemia were established by ligaturing the left anterior descending coronary artery; for the pressing group, “Xinshu” (B15) was pressed the next day after operation, and for the other groups, only bundle fixation was taken. Each pressing or bundle fixation lasted for 15 min, once a day for the consecutive 7 d. The ECG of rats were checked by the small animal ECG machine before modeling, after modeling, and after intervention; the cardiac troponin I (cTnI), creatine kinase MB isoenzymes (CK-MB), and layered double hydroxide (LDH) in serum, and the myocardial adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) were tested by the enzyme-linked immunosorbent assay. Results (1) The J-point amplitudes after modeling were higher than those before modeling (P<0.05); after intervention, compared with model group, the J-point amplitudes of pressing group decreased (P<0.05). (2) HE staining showed the ischemia areas of left ventricular wall for the model group were larger than the other two groups, and the ischemia for pressing group was better than model group. (3) Compared with the sham-operated group, model group showed a significant content increase of cTnI, CK-MB, LDH in the serum (P<0.01), a significant decrease of ATP content and EC value of rats (P<0.01), and a significant content increase of the ADP and AMP in rats (P<0.01); compared with model group, pressing group showed a significant content decrease of cTnI, CK-MB, LDH in serum (P<0.01), a significant increase of ATP and energy charge (EC), and the significant decrease of AMP (P<0.01). Conclusion Pressing "Xinshu" (B15) may effectively protect the ischemia myocardium, and the possible mechanism is to improve the myocardial energy metabolism and the condition of ischemia and hypoxia by decreasing the ADP and AMP of myocardium and increasing the ATP and EC, thus protecting the myocardium. |
| Key words: pressing myocardial ischemia rats "Xinshu" (B15) energy metabolism Tuina adenosine triphosphate |
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